{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T03:55:06Z","timestamp":1792900506615,"version":"4.5.0"},"reference-count":109,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T00:00:00Z","timestamp":1706486400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Poultry production occupies an important place in the economy of any country. High broiler production in recent years has badly affected its profitability due to bad feed quality, excessive use of chemotherapeutic agents, emergence of diverse pathogens, and the deficiencies in management practices during rearing cycle. Microbiological improvement of the meat quality using potential probiotics can be beneficial for broiler farming. Present study was initiated to isolate chicken gastrointestinal tract (GIT) bacteria with probiotic potential. To isolate probiotics from chicken gut, alimentary canal of chickens of known sizes and ages was suspended in ringers soln. Under shaking conditions for overnight followed by serial dilutions of ringers soln. Bacterial isolates were analyzed via growth curve analysis, biochemical testing using RapID\u2122 NF Plus Panel kit, molecular characterization, antimicrobial activity assay, antibiotic sensitivity assay, GIT adherence assay, bile salt and gastric acid resistant assay, and cholesterol assimilation assay. Four bacteria isolated in present study were identified as <jats:italic>Limosilactobacillus antri<\/jats:italic> strain PUPro1, <jats:italic>Lactobacillus delbrueckii<\/jats:italic> strain PUPro2, <jats:italic>Lacticaseibacillus casei<\/jats:italic> strain PUPro3, and <jats:italic>Ligilactobacillus salivarius<\/jats:italic> strain PUPro4. <jats:italic>L. delbrueckii<\/jats:italic> strain PUPro2 grew extremely fast. All isolates exhibited exceptional resistance to increasing concentrations of NaCl and bile salts with value of <jats:italic>p<\/jats:italic> &amp;gt;0.5. <jats:italic>L. delbrueckii<\/jats:italic> strain PUPro2 adhered to chicken ileum epithelial cells and demonstrated the highest viable counts of 320 colony forming units (CFUs). Antagonistic action was found in all isolates against <jats:italic>P. aeruginosa<\/jats:italic>, <jats:italic>B. subtilis<\/jats:italic>, <jats:italic>B. proteus<\/jats:italic>, and <jats:italic>S. aureus<\/jats:italic>, with value of <jats:italic>p<\/jats:italic> &amp;gt;0.5. Antibiotic susceptibility testing showed sensitivity to all the antibiotics used. Cholesterol assimilation was detected in all bacteria, with values ranging from 216.12 to 192.2\u2009mg\/dL. All isolates exhibited \u03b3-hemolysis. In future, these bacteria might be tested for their impact on broilers meat quality and growth and can be recommended for their use as supplements for broilers diet with positive impact on poultry production.<\/jats:p>","DOI":"10.3389\/fmicb.2024.1278439","type":"journal-article","created":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T13:56:45Z","timestamp":1706536605000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":21,"title":["Isolation and in vitro assessment of chicken gut microbes for probiotic potential"],"prefix":"10.3389","volume":"15","author":[{"given":"Fatima","family":"Shahbaz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatima","family":"Muccee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aansa","family":"Shahab","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sher Zaman","family":"Safi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suliman Yousef","family":"Alomar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul","family":"Qadeer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,1,29]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1835","DOI":"10.1111\/jpn.13454","article-title":"Probiotics in poultry feed: a comprehensive review","volume":"104","author":"Abd El-Hack","year":"2020","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"954","DOI":"10.3390\/microorganisms10050954","article-title":"Identification, biochemical characterization, and safety attributes of locally isolated Lactobacillus fermentum from Bubalus bubalis (buffalo) milk as a probiotic","volume":"10","author":"Abid","year":"2022","journal-title":"Microorganisms"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"716","DOI":"10.1016\/j.chom.2018.05.003","article-title":"Gut microbiota regulation of tryptophan metabolism in health and disease","volume":"23","author":"Agus","year":"2018","journal-title":"Cell Host Microbe"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"2164665","DOI":"10.1080\/23322039.2022.2164665","article-title":"Islamic finance education: current state and challenges for Pakistan","volume":"11","author":"Akbar","year":"2023","journal-title":"Cogent. Econ. Finance"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"419","DOI":"10.57041\/pjs.v75i02.8","article-title":"Effect of dietary supplementation of animal blood plasma powder on production performance, dressed weight and immune response of broiler","volume":"75","author":"Alam","year":"2023","journal-title":"Pak. J. Sci."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1016\/j.jbiosc.2014.01.015","article-title":"Isolation of potential probiotic Lactobacillus oris HMI68 from mother's milk with cholesterol-reducing property","volume":"118","author":"Anandharaj","year":"2014","journal-title":"J. Biosci. Bioeng."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"319","DOI":"10.14202\/vetworld.2021.319-328","article-title":"The use of probiotics in animal feeding for safe production and as potential alternatives to antibiotics","volume":"14","author":"Ars\u00e8ne","year":"2021","journal-title":"Vet. World."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"361","DOI":"10.3389\/fvets.2020.00361","article-title":"A value chain approach to characterize the chicken sub-sector in Pakistan","volume":"7","author":"Aslam","year":"2020","journal-title":"Front. Vet. Sci."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"100331","DOI":"10.1016\/j.animal.2021.100331","article-title":"Production factors affecting poultry carcass and meat quality attributes","volume":"16","author":"Ba\u00e9za","year":"2022","journal-title":"Animal"},{"key":"ref10","doi-asserted-by":"publisher","first-page":"5883","DOI":"10.1007\/s11033-019-05022-z","article-title":"Next generation sequencing, biochemical characterization, metabolic pathway analysis of novel probiotic Pediococcus acidilactici NCDC 252 and it\u2019s evolutionary relationship with other lactic acid bacteria","volume":"46","author":"Bansal","year":"2019","journal-title":"Mol. Biol. Rep."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"650","DOI":"10.3389\/fmicb.2016.00650","article-title":"Performance of 16s rDNA primer pairs in the study of rhizosphere and endosphere bacterial microbiomes in metabarcoding studies","volume":"7","author":"Beckers","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref12","first-page":"13","article-title":"Acid and bile tolerance of Lactobacillus thermotolerans, a novel species isolated from chicken feces","volume":"40","author":"Boonkumklao","year":"2006","journal-title":"Agric. Nat. Resour."},{"key":"ref13","first-page":"31","article-title":"Effect of fibrous materials inclusion on the solid-state fermentation of post harvested wastes of Solanum tuberosum","volume":"55","author":"Borras","year":"2021","journal-title":"Cuba. J. Agric. Sci."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"100310","DOI":"10.1016\/j.japr.2022.100310","article-title":"Poultry industry paradigms: connecting the dots","volume":"32","author":"Castro","year":"2023","journal-title":"J. Appl. Poult. Res."},{"key":"ref15","doi-asserted-by":"publisher","first-page":"683","DOI":"10.5897\/AJMR12.2196","article-title":"Isolation and functional study of potentially probiotic lactobacilli from Taiwan traditional paocai","volume":"7","author":"Chang","year":"2013","journal-title":"Afr. J. Microbiol. Res."},{"key":"ref16","doi-asserted-by":"publisher","first-page":"912","DOI":"10.1007\/s12602-022-09923-7","article-title":"Effects of dietary supplementation with Lactobacillus acidophilus and Bacillus subtilis on mucosal immunity and intestinal barrier are associated with its modulation of gut metabolites and microbiota in late-phase laying hens","volume":"15","author":"Chen","year":"2023","journal-title":"Probiot.Antimicrob. Proteins"},{"key":"ref17","doi-asserted-by":"publisher","first-page":"1387","DOI":"10.1007\/s12602-021-09777-5","article-title":"Investigation of antibacterial activity and probiotic properties of strains belonging to Lactobacillus and Bifidobacterium genera for their potential application in functional food and feed products","volume":"13","author":"Cizeikiene","year":"2021","journal-title":"Probiot. Antimicrob. Proteins"},{"key":"ref18","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1016\/j.foodres.2006.11.007","article-title":"In vitro analysis of probiotic strain combinations to inhibit pathogen adhesion to human intestinal mucus","volume":"40","author":"Collado","year":"2007","journal-title":"Food Res. Int."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1016\/S0168-1605(02)00425-7","article-title":"Screening for enterocins and detection of hemolysin and vancomycin resistance in enterococci of different origins","volume":"84","author":"De Vuyst","year":"2003","journal-title":"Int. J. Food Microbiol."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1016\/j.gfs.2017.12.004","article-title":"Assessing current and future meat and fish consumption in sub-Sahara Africa: learnings from FAO food balance sheets and LSMS household survey data","volume":"16","author":"Desiere","year":"2018","journal-title":"Glob. Food Sec."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"e0212079","DOI":"10.1371\/journal.pone.0223971","article-title":"Effects of Lactobacillus plantarum 15-1 and fructooligosaccharides on the response of broilers to pathogenic Escherichia coli O78 challenge","volume":"14","author":"Ding","year":"2019","journal-title":"PLoS One"},{"key":"ref22","doi-asserted-by":"publisher","first-page":"e0192978","DOI":"10.1371\/journal.pone.0192978","article-title":"Selection and characterization of probiotic lactic acid bacteria and its impact on growth, nutrient digestibility, health and antioxidant status in weaned piglets","volume":"13","author":"Dowarah","year":"2018","journal-title":"PLoS One"},{"key":"ref23","doi-asserted-by":"publisher","first-page":"883","DOI":"10.1080\/00439339.2021.1960239","article-title":"Physiological and immunological benefits of probiotics and their impacts in poultry productivity","volume":"77","author":"Ebeid","year":"2021","journal-title":"World Poultry Sci. J."},{"key":"ref24","doi-asserted-by":"crossref","DOI":"10.17504\/protocols.io.x54v9ykd4g3e\/v2","volume-title":"Preparation of Bacteria glycerol stocks","author":"Fadanka","year":"2022"},{"key":"ref25","first-page":"713","article-title":"Effective microbes (EM) supplementation effect on feed intake digestibility and live weight changes of Washera sheep fed wheat straw","volume":"4","author":"Fekadie","year":"2022","journal-title":"Iran. J. Appl. Anim. Sci."},{"key":"ref26","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/j.scib.2017.01.001","article-title":"Effect of feeding Lactobacillus plantarum P-8 on the faecal microbiota of broiler chickens exposed to lincomycin","volume":"62","author":"Gao","year":"2017","journal-title":"Sci. Bull."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1016\/j.rvsc.2016.08.009","article-title":"Isolation, characterization and evaluation of probiotic lactic acid bacteria for potential use in animal production","volume":"108","author":"Garc\u00eda-Hern\u00e1ndez","year":"2016","journal-title":"Res. Vet. Sci."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"384","DOI":"10.1128\/jcm.1.4.384-389.1975","article-title":"Characteristics of yellow-pigmented nonfermentative bacilli (groups Ve-1 and Ve-2) encountered in clinical bacteriology","volume":"1","author":"Gilardi","year":"1975","journal-title":"J. Clin. Microbiol."},{"key":"ref29","doi-asserted-by":"publisher","first-page":"pdb.top100388","DOI":"10.1101\/pdb.top100388","article-title":"Analysis of DNA by agarose gel electrophoresis","volume":"2019","author":"Green","year":"2019","journal-title":"Cold Spring Harbor Protoc"},{"key":"ref30","doi-asserted-by":"publisher","first-page":"4","DOI":"10.3389\/fmicb.2013.00202","article-title":"Antibiotic resistance in probiotic bacteria","author":"Gueimonde Fern\u00e1ndez","year":"2013","journal-title":"Front. Microbiol."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"116","DOI":"10.4103\/JOC.JOC_110_18","article-title":"DNA extraction and polymerase chain reaction","volume":"36","author":"Gupta","year":"2019","journal-title":"J. Cytol."},{"key":"ref32","doi-asserted-by":"publisher","first-page":"e0280412","DOI":"10.1371\/journal.pone.0280412","article-title":"Identification and characterization of probiotics isolated from indigenous chicken (Gallus domesticus) of Nepal","volume":"18","author":"Gupta","year":"2023","journal-title":"PLoS One"},{"key":"ref33","doi-asserted-by":"publisher","first-page":"348","DOI":"10.3390\/ani13030348","article-title":"Dynamic changes in the gut microbiota and metabolites during the growth of Hainan Wenchang chickens","volume":"13","author":"He","year":"2023","journal-title":"Animals"},{"key":"ref34","doi-asserted-by":"publisher","first-page":"100287","DOI":"10.1016\/j.animal.2021.100287","article-title":"Trends for meat, milk and egg consumption for the next decades and the role played by livestock systems in the global production of proteins","volume":"15","author":"Henchion","year":"2021","journal-title":"Animal"},{"key":"ref35","volume-title":"Bergey's manual of systematic bacteriology","author":"Holt","year":"1984"},{"key":"ref36","first-page":"39","article-title":"Isolation, identification and analysis of probiotic properties of Lactobacillus spp. from selective regional yoghurts","volume":"5","author":"Hoque","year":"2010","journal-title":"World J. Dairy Food Sci."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"644","DOI":"10.3390\/ani9090644","article-title":"Effects of feeding different postbiotics produced by Lactobacillus plantarum on growth performance, carcass yield, intestinal morphology, gut microbiota composition, immune status, and growth gene expression in broilers under heat stress","volume":"9","author":"Humam","year":"2019","journal-title":"Animals"},{"key":"ref38","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1136\/jcp.30.3.275","article-title":"API ZYM: a simple rapid system for the detection of bacterial enzymes","volume":"30","author":"Humble","year":"1977","journal-title":"J. Clin. Pathol."},{"key":"ref39","first-page":"1115","article-title":"Screening of Lactobacillus strains against Salmonella both isolated from Malaysian free-range chicken intestine for use as probiotic","volume":"40","author":"Hutari","year":"2011","journal-title":"Sains Malays"},{"key":"ref109","first-page":"209","article-title":"The role of probiotics in the poultry industry","volume-title":"Urgent issues of agricultural science, production and education","author":"Ivanova","year":"2019"},{"key":"ref40","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1016\/j.ijid.2020.05.034","article-title":"Isolation and identification of potential probiotic Lactobacillus species from feces of infants in Southwest Iran","volume":"96","author":"Jomehzadeh","year":"2020","journal-title":"Int. J. Infect. Dis."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"198","DOI":"10.3390\/microorganisms3020198","article-title":"Comparison of microbiological and probiotic characteristics of lactobacilli isolates from dairy food products and animal rumen contents","volume":"3","author":"Jose","year":"2015","journal-title":"Microorganisms"},{"key":"ref42","doi-asserted-by":"publisher","first-page":"e00600","DOI":"10.1128\/AEM.00600-20","article-title":"The gut microbiota of laying hens and its manipulation with prebiotics and probiotics to enhance gut health and food safety","volume":"86","author":"Khan","year":"2020","journal-title":"Appl. Environ. Microbiol."},{"key":"ref43","first-page":"245","article-title":"Rapid diagnosis of Enterobacteriaceae: I. Detection of bacterial glycosidases","volume":"84","author":"Kilian","year":"1976","journal-title":"Acta Pathol. Microbiol. Scand. Sect. B Microbiol."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1016\/j.anaerobe.2015.03.004","article-title":"Characterization of Lactobacillus isolated from dairy samples for probiotic properties","volume":"33","author":"Kumar","year":"2015","journal-title":"Anaerobe"},{"key":"ref45","doi-asserted-by":"publisher","first-page":"1882","DOI":"10.3390\/nu15081882","article-title":"Antidiabetic activity of potential probiotics limosilactobacillus spp., levilactobacillus spp., and lacticaseibacillus spp. isolated from fermented sugarcane juice: a comprehensive in vitro and in silico study","volume":"15","author":"Kumari Vb","year":"2023","journal-title":"Nutrients"},{"key":"ref46","doi-asserted-by":"publisher","first-page":"2033","DOI":"10.1099\/ijs.0.63684-0","article-title":"Kordiimonas gwangyangensis gen. Nov., sp. nov., a marine bacterium isolated from marine sediments that forms a distinct phyletic lineage (Kordiimonadales Ord. Nov.) in the \u2018Alphaproteobacteria\u2019","volume":"55","author":"Kwon","year":"2005","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref47","volume-title":"Integrative analysis of the microbiome and metabolome of broiler intestine: insights into the mechanisms of probiotic action as an antibiotic substitute","author":"Li","year":"2023"},{"key":"ref48","doi-asserted-by":"publisher","first-page":"102165","DOI":"10.1016\/j.psj.2022.102165","article-title":"Integrated omics analysis reveals differences in gut microbiota and gut-host metabolite profiles between obese and lean chickens","volume":"101","author":"Liu","year":"2022","journal-title":"Poultry Sci."},{"key":"ref49","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12934-020-1289-4","article-title":"Surface components and metabolites of probiotics for regulation of intestinal epithelial barrier","volume":"19","author":"Liu","year":"2020","journal-title":"Microb. Cell Factories"},{"key":"ref50","doi-asserted-by":"publisher","first-page":"e3998","DOI":"10.3791\/3998","article-title":"Polymerase chain reaction: basic protocol plus troubleshooting and optimization strategies","author":"Lorenz","year":"2012","journal-title":"J. Vis. Exp."},{"key":"ref51","doi-asserted-by":"publisher","first-page":"3531","DOI":"10.3390\/ijms10083531","article-title":"The role of probiotics in the poultry industry","volume":"10","author":"Lutful","year":"2009","journal-title":"Int. J. Mol. Sci."},{"key":"ref52","volume-title":"Biochemical tests for identification of medical bacteria","author":"MacFaddin","year":"2000"},{"key":"ref53","doi-asserted-by":"publisher","first-page":"2175","DOI":"10.1128\/JCM.00025-14","article-title":"Evaluation of the Remel RapID NF plus rapid biochemical method for identification of Burkholderia pseudomallei","volume":"52","author":"Maloney","year":"2014","journal-title":"J. Clin. Microbiol."},{"key":"ref54","doi-asserted-by":"publisher","first-page":"1284","DOI":"10.3390\/ani13081284","article-title":"In vitro characterization of probiotic potential of Limosilactobacillus fermentum against Salmonella gallinarum causing fowl typhoid","volume":"13","author":"Mehmood","year":"2023","journal-title":"Animals"},{"key":"ref55","doi-asserted-by":"crossref","DOI":"10.21203\/rs.3.rs-2697471\/v1","article-title":"Isolation, characterization and in-vitro evaluation of potential probiotic lactic acid bacteria isolated from chicken gut","author":"Melese","year":"2023"},{"key":"ref56","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1016\/j.jff.2014.05.002","article-title":"Cholesterol reduction mechanisms and fatty acid composition of cellular membranes of probiotic lactobacilli and Bifidobacteria","volume":"9","author":"Miremadi","year":"2014","journal-title":"J. Funct. Foods"},{"key":"ref57","doi-asserted-by":"publisher","first-page":"100906","DOI":"10.1016\/j.psj.2020.11.073","article-title":"Effects of dietary supplementation of a probiotic (Bacillus subtilis) on bone mass and meat quality of broiler chickens","volume":"100","author":"Mohammed","year":"2021","journal-title":"Poult. Sci."},{"key":"ref58","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1099\/00221287-61-1-9","article-title":"Pyrrolidonyl peptidase in bacteria: a new colrimetric test for differentiation of enterobacteriaceae","volume":"61","author":"Mulczyk","year":"1970","journal-title":"J. Gen. Microbiol."},{"key":"ref59","doi-asserted-by":"publisher","first-page":"1337","DOI":"10.1007\/s11274-009-0020-8","article-title":"Probiotic potential of lactic acid bacteria isolated from chicken gastrointestinal digestive tract","volume":"25","author":"Musikasang","year":"2009","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref60","doi-asserted-by":"publisher","first-page":"466","DOI":"10.1016\/0003-2697(76)90227-X","article-title":"New chromogenic substrates for X-prolyl dipeptidyl-aminopeptidase","volume":"74","author":"Nagatsu","year":"1976","journal-title":"Anal. Biochem."},{"key":"ref61","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1080\/08905436.2016.1269289","article-title":"Molecular characterization of antimicrobial Lactobacillus isolates and evaluation of their probiotic characteristics in vitro for use in poultry","volume":"31","author":"Nallala","year":"2017","journal-title":"Food Biotechnol."},{"key":"ref62","doi-asserted-by":"publisher","first-page":"3975","DOI":"10.20546\/ijcmas.2017.607.411","article-title":"Isolation and screening of lactic acid bacteria for acidic pH and bile tolerance","volume":"6","author":"Nawaz","year":"2017","journal-title":"Int. J. Curr. Microbiol. Appl. Sci."},{"key":"ref63","doi-asserted-by":"publisher","first-page":"759","DOI":"10.2217\/fmb.10.35","article-title":"Probiotic properties of Lactobacillus salivarius and closely related Lactobacillus species","volume":"5","author":"Neville","year":"2010","journal-title":"Future Microbiol."},{"key":"ref64","doi-asserted-by":"publisher","first-page":"876725","DOI":"10.3389\/fvets.2022.876725","article-title":"Bacillus probiotics as alternatives to in-feed antibiotics and its influence on growth, serum chemistry, antioxidant status, intestinal histomorphology, and lesion scores in disease-challenged broiler chickens","volume":"9","author":"Ogbuewu","year":"2022","journal-title":"Front. Vet. Sci."},{"key":"ref65","first-page":"5","article-title":"Tolerance of Lactobacillus and Bifidobacterium strains to low pH, bile salts and digestive enzymes","volume":"8","author":"Olejnik","year":"2005","journal-title":"Food Sci. Technol."},{"key":"ref66","doi-asserted-by":"publisher","first-page":"50","DOI":"10.51406\/jagse.v13i1.1210","article-title":"Phenotypic characterization and in vitro screening of lactic acid bacteria from goat milk for probiotic USE","volume":"13","author":"Oloyede","year":"2013","journal-title":"J. Agric. Sci. Environ."},{"key":"ref67","doi-asserted-by":"publisher","DOI":"10.5455\/ajvs.290499","article-title":"In vitro study of potential probiotic lactic acid bacteria isolated from the gut of chickens in Abeokuta, Nigeria","volume":"58","author":"Oyewole","year":"2018","journal-title":"Alex. J. Vet. Sci"},{"key":"ref68","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1016\/j.foodcont.2013.09.055","article-title":"Probiotic potential, antimicrobial and antioxidant activities of Enterococcus durans strain LAB18s","volume":"37","author":"Pieniz","year":"2014","journal-title":"Food Control"},{"key":"ref69","doi-asserted-by":"publisher","first-page":"1011","DOI":"10.1590\/0001-3765201620150071","article-title":"Probiotic level effects on growth performance, carcass traits, blood parameters, cecal microbiota, and immune response of broilers","volume":"88","author":"Pourakbari","year":"2016","journal-title":"An. Acad. Bras. Ci\u00eanc."},{"key":"ref70","first-page":"545","article-title":"Screening of lactic acid bacteria of different origin for their probiotic potential","volume":"7","author":"Puniya","year":"2016","journal-title":"J. Food Process. Technol."},{"key":"ref71","doi-asserted-by":"publisher","first-page":"424","DOI":"10.3389\/fmicb.2019.00424","article-title":"Probiotics: reiterating what they are and what they are not","volume":"10","author":"Reid","year":"2019","journal-title":"Front. 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Rep."},{"key":"ref75","doi-asserted-by":"publisher","first-page":"825","DOI":"10.3390\/microorganisms9040825","article-title":"Probiotic properties and immunomodulatory activity of Lactobacillus strains isolated from dairy products","volume":"9","author":"Rocha-Ram\u00edrez","year":"2021","journal-title":"Microorganisms"},{"key":"ref76","doi-asserted-by":"publisher","first-page":"67","DOI":"10.30466\/vrf.2018.84395.2110","article-title":"Evaluation of lactic acid bacteria isolated from poultry feces as potential probiotic and its in vitro competitive activity against Salmonella typhimurium","volume":"11","author":"Salehizadeh","year":"2020","journal-title":"Vet. Res. 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Dairy Sci."},{"key":"ref80","doi-asserted-by":"publisher","first-page":"605","DOI":"10.1038\/s41575-019-0173-3","article-title":"Probiotics and prebiotics in intestinal health and disease: from biology to the clinic","volume":"16","author":"Sanders","year":"2019","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref81","article-title":"Antimicrobial activity and adhesion ability of indigenous lactic acid bacteria isolated from goat milk","volume":"21","author":"Setyawardani","year":"2014","journal-title":"Int. J. Food Res."},{"key":"ref82","doi-asserted-by":"publisher","first-page":"8","DOI":"10.54987\/jobimb.v7i2.476","article-title":"Probiotic properties of Lactobacillus isolates from chicken intestines","volume":"7","author":"Shamsudin","year":"2019","journal-title":"J. Biochem. Microbiol. 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