{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T01:32:33Z","timestamp":1773797553400,"version":"3.50.1"},"reference-count":63,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T00:00:00Z","timestamp":1709078400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Timely initiation of chromosomal DNA replication in <jats:italic>Escherichia coli<\/jats:italic> is achieved by cell cycle-coordinated regulation of the replication origin, <jats:italic>oriC<\/jats:italic>, and the replication initiator, ATP-DnaA. Cellular levels of ATP-DnaA increase and peak at the time for initiation at <jats:italic>oriC<\/jats:italic>, after which hydrolysis of DnaA-bound ATP causes those to fall, yielding initiation-inactive ADP-DnaA. This hydrolysis is facilitated by the chromosomal locus <jats:italic>datA<\/jats:italic> located downstream of the tRNA-Gly (<jats:italic>glyV-X-Y<\/jats:italic>) operon, which possesses a cluster of DnaA-binding sequences and a single binding site (IBS) for the DNA bending protein IHF (integration host factor). While IHF binding activates the <jats:italic>datA<\/jats:italic> function and is regulated to occur specifically at post-initiation time, the underlying regulatory mechanisms remain obscure. Here, we demonstrate that <jats:italic>datA<\/jats:italic>-IHF binding at pre-initiation time is down-regulated depending on the read-through transcription of <jats:italic>datA<\/jats:italic> IBS initiated at the <jats:italic>glyV-X-Y<\/jats:italic> promoter. During the cell cycle, the level of read-through transcription, but not promoter activity, fluctuated in a manner inversely related to <jats:italic>datA<\/jats:italic>-IHF binding. Transcription from the <jats:italic>glyV-X-Y<\/jats:italic> promoter was predominantly interrupted at <jats:italic>datA<\/jats:italic> IBS by IHF binding. The terminator\/attenuator sequence of the <jats:italic>glyV-X-Y<\/jats:italic> operon, as well as DnaA binding within <jats:italic>datA<\/jats:italic> overall, contributed to attenuation of transcription upstream of <jats:italic>datA<\/jats:italic> IBS, preserving the timely fluctuation of read-through transcription. These findings provide a mechanistic insight of tRNA transcription-dependent <jats:italic>datA<\/jats:italic>-IHF regulation, in which an unidentified factor is additionally required for the timely <jats:italic>datA<\/jats:italic>-IHF dissociation, and support the significance of <jats:italic>datA<\/jats:italic> for controlling the cell cycle progression as a connecting hub of tRNA production and replication initiation.<\/jats:p>","DOI":"10.3389\/fmicb.2024.1360108","type":"journal-article","created":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T04:52:26Z","timestamp":1709095946000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Read-through transcription of tRNA underlies the cell cycle-dependent dissociation of IHF from the DnaA-inactivating sequence datA"],"prefix":"10.3389","volume":"15","author":[{"given":"Kazutoshi","family":"Kasho","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryuji","family":"Sakai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kosuke","family":"Ito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wataru","family":"Nakagaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rion","family":"Satomura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takafumi","family":"Jinnouchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shogo","family":"Ozaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsutomu","family":"Katayama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2024,2,28]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"39236","DOI":"10.1074\/jbc.M606363200","article-title":"Indirect recognition in sequence-specific DNA binding by Escherichia coli integration host factor: The role of DNA deformation energy.","volume":"281","author":"Aeling","year":"2006","journal-title":"J. Biol. Chem."},{"key":"B2","doi-asserted-by":"publisher","first-page":"6361","DOI":"10.1128\/JB.181.20.6361-6370.1999","article-title":"Growth phase-dependent variation in protein composition of the Escherichia coli nucleoid.","volume":"181","author":"Ali Azam","year":"1999","journal-title":"J. Bacteriol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1016\/0167-4781(90)90184-4","article-title":"FIS-dependent trans-activation of tRNA and rRNA operons of Escherichia coli.","volume":"1050","author":"Bosch","year":"1990","journal-title":"Biochim. Biophys. Acta"},{"key":"B4","doi-asserted-by":"publisher","first-page":"5833","DOI":"10.1002\/j.1460-2075.1995.tb00271.x","article-title":"Cell cycle-specific changes in nucleoprotein complexes at a chromosomal replication origin.","volume":"14","author":"Cassler","year":"1995","journal-title":"EMBO J."},{"key":"B5","doi-asserted-by":"publisher","first-page":"6640","DOI":"10.1073\/pnas.120163297","article-title":"One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.","volume":"97","author":"Datsenko","year":"2000","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B6","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1038\/nrmicro2261","article-title":"Bacterial nucleoid-associated proteins, nucleoid structure and gene expression.","volume":"8","author":"Dillon","year":"2010","journal-title":"Nat. Rev. Microbiol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1038\/nature10455","article-title":"DNA stretching by bacterial initiators promotes replication origin opening.","volume":"478","author":"Duderstadt","year":"2011","journal-title":"Nature"},{"key":"B8","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1006\/jmbi.1993.1165","article-title":"Growth-rate-dependent accumulation of twelve tRNA species in Escherichia coli.","volume":"230","author":"Emilsson","year":"1993","journal-title":"J. Mol. Biol."},{"key":"B9","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nsmb1115","article-title":"Structural basis for ATP-dependent DnaA assembly and replication-origin remodeling.","volume":"13","author":"Erzberger","year":"2006","journal-title":"Nat. Struct. Mol. Biol."},{"key":"B10","doi-asserted-by":"publisher","first-page":"1018","DOI":"10.1111\/j.1365-2958.2008.06585.x","article-title":"DnaA protein interacts with RNA polymerase and partially protects it from the effect of rifampicin.","volume":"71","author":"Fl\u00e5tten","year":"2009","journal-title":"Mol. Microbiol."},{"key":"B11","doi-asserted-by":"publisher","first-page":"607","DOI":"10.1007\/s00294-016-0671-6","article-title":"Control of bacterial chromosome replication by non-coding regions outside the origin.","volume":"63","author":"Frimodt-M\u00f8ller","year":"2017","journal-title":"Curr. Genet."},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.01011","article-title":"Control regions for chromosome replication are conserved with respect to sequence and location among Escherichia coli strains.","volume":"6","author":"Frimodt-M\u00f8ller","year":"2015","journal-title":"Front. Microbiol."},{"key":"B13","doi-asserted-by":"publisher","first-page":"2077","DOI":"10.1093\/nar\/gkg309","article-title":"Structural basis of replication origin recognition by the DnaA protein.","volume":"31","author":"Fujikawa","year":"2003","journal-title":"Nucleic Acids Res."},{"key":"B14","doi-asserted-by":"publisher","first-page":"1221","DOI":"10.1101\/gad.1775809","article-title":"Specific genomic sequences of E. coli promote replicational initiation by directly reactivating ADP-DnaA.","volume":"23","author":"Fujimitsu","year":"2009","journal-title":"Genes Dev."},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.732270","article-title":"Blocking, bending, and binding: Regulation of initiation of chromosome replication during the Escherichia coli cell cycle by transcriptional modulators that interact with origin DNA.","volume":"12","author":"Grimwade","year":"2021","journal-title":"Front. Microbiol."},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.00319","article-title":"The DnaA Tale.","volume":"9","author":"Hansen","year":"2018","journal-title":"Front. Microbiol."},{"key":"B17","doi-asserted-by":"publisher","first-page":"1131","DOI":"10.1074\/jbc.ra120.014235","article-title":"DnaB helicase is recruited to the replication initiation complex via binding of DnaA domain I to the lateral surface of the DnaB N-terminal domain.","volume":"295","author":"Hayashi","year":"2020","journal-title":"J. Biol. Chem."},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/j.mex.2019.12.001","article-title":"Optimization of overlap extension PCR for efficient transgene construction.","volume":"7","author":"Hilgarth","year":"2020","journal-title":"MethodsX"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00590","article-title":"Nucleoid associated proteins: The small organizers that help to cope with stress.","volume":"11","author":"Ho\u0142\u00f3wka","year":"2020","journal-title":"Front. Microbiol."},{"key":"B20","doi-asserted-by":"crossref","first-page":"5033","DOI":"10.1016\/S0021-9258(19)43667-3","article-title":"Small ribonucleic acids of Escherichia coli. II. Noncoordinate accumulation during stringent control.","volume":"248","author":"Ikemura","year":"1973","journal-title":"J. Biol. Chem."},{"key":"B21","doi-asserted-by":"publisher","first-page":"1015","DOI":"10.1111\/gtc.12395","article-title":"Chromosomal location of the DnaA-reactivating sequence DARS2 is important to regulate timely initiation of DNA replication in Escherichia coli.","volume":"21","author":"Inoue","year":"2016","journal-title":"Genes Cells"},{"key":"B22","doi-asserted-by":"publisher","first-page":"256","DOI":"10.1038\/s41579-020-00470-y","article-title":"The stringent response and physiological roles of (pp)pGpp in bacteria.","volume":"19","author":"Irving","year":"2021","journal-title":"Nat. Rev. Microbiol."},{"key":"B23","doi-asserted-by":"publisher","first-page":"1907","DOI":"10.1038\/s41564-019-0500-z","article-title":"Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria.","volume":"4","author":"Ju","year":"2019","journal-title":"Nat. Microbiol."},{"key":"B24","doi-asserted-by":"publisher","first-page":"936","DOI":"10.1073\/pnas.1212070110","article-title":"DnaA binding locus datA promotes DnaA-ATP hydrolysis to enable cell cycle-coordinated replication initiation.","volume":"110","author":"Kasho","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B25","doi-asserted-by":"publisher","first-page":"13134","DOI":"10.1093\/nar\/gku1051","article-title":"Timely binding of IHF and Fis to DARS2 regulates ATP-DnaA production and replication initiation.","volume":"42","author":"Kasho","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.697712","article-title":"Whole-genome analysis reveals that the nucleoid protein IHF predominantly binds to the replication origin oriC specifically at the time of initiation.","volume":"12","author":"Kasho","year":"2021","journal-title":"Front. Microbiol."},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241411572","article-title":"IHF and Fis as Escherichia coli cell cycle regulators: Activation of the replication origin oriC and the regulatory cycle of the DnaA initiator.","volume":"24","author":"Kasho","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"B28","doi-asserted-by":"publisher","first-page":"1251","DOI":"10.1074\/jbc.M116.762815","article-title":"Cooperative DnaA binding to the negatively supercoiled datA locus stimulates DnaA-ATP hydrolysis.","volume":"292","author":"Kasho","year":"2017","journal-title":"J. Biol. Chem."},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.02496","article-title":"The DnaA cycle in Escherichia coli: Activation, function and inactivation of the initiator protein.","volume":"8","author":"Katayama","year":"2017","journal-title":"Front. Microbiol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1038\/nrmicro2314","article-title":"Regulation of the replication cycle: Conserved and diverse regulatory systems for DnaA and oriC.","volume":"8","author":"Katayama","year":"2010","journal-title":"Nat. Rev. Microbiol."},{"key":"B31","doi-asserted-by":"publisher","first-page":"27420","DOI":"10.1074\/jbc.M502764200","article-title":"Formation of an ATP-DnaA-specific initiation complex requires DnaA Arginine 285, a conserved motif in the AAA+ protein family.","volume":"280","author":"Kawakami","year":"2005","journal-title":"J. Biol. Chem."},{"key":"B32","doi-asserted-by":"publisher","first-page":"1310","DOI":"10.1111\/j.1365-2958.2006.05450.x","article-title":"The exceptionally tight affinity of DnaA for ATP\/ADP requires a unique aspartic acid residue in the AAA+ sensor 1 motif.","volume":"62","author":"Kawakami","year":"2006","journal-title":"Mol. Microbiol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"25038","DOI":"10.1074\/jbc.M109.002717","article-title":"DiaA dynamics are coupled with changes in initial origin complexes leading to helicase loading.","volume":"284","author":"Keyamura","year":"2009","journal-title":"J. Biol. Chem."},{"key":"B34","doi-asserted-by":"publisher","first-page":"1137","DOI":"10.1046\/j.1365-2958.1996.453983.x","article-title":"A novel DnaA protein-binding site at 94.7 min on the Escherichia coli chromosome.","volume":"19","author":"Kitagawa","year":"1996","journal-title":"Mol. Microbiol."},{"key":"B35","doi-asserted-by":"publisher","first-page":"3032","DOI":"10.1101\/gad.12.19.3032","article-title":"Negative control of replication initiation by a novel chromosomal locus exhibiting exceptional affinity for Escherichia coli DnaA protein.","volume":"12","author":"Kitagawa","year":"1998","journal-title":"Genes Dev."},{"key":"B36","doi-asserted-by":"publisher","first-page":"579","DOI":"10.1016\/0022-2836(90)90224-A","article-title":"Genomic organization and physical mapping of the transfer RNA genes in Escherichia coli K12.","volume":"212","author":"Komine","year":"1990","journal-title":"J. Mol. Biol."},{"key":"B37","doi-asserted-by":"publisher","first-page":"12820","DOI":"10.1093\/nar\/gkab1171","article-title":"Negative feedback for DARS2\u2013Fis complex by ATP\u2013DnaA supports the cell cycle-coordinated regulation for chromosome replication.","volume":"49","author":"Miyoshi","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23074008","article-title":"Small prokaryotic DNA-binding proteins protect genome integrity throughout the life cycle.","volume":"23","author":"Molan","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"B39","doi-asserted-by":"publisher","first-page":"921","DOI":"10.1128\/jb.174.3.921-929.1992","article-title":"FIS-dependent trans activation of stable RNA operons of Escherichia coli under various growth conditions.","volume":"174","author":"Nilsson","year":"1992","journal-title":"J. Bacteriol."},{"key":"B40","doi-asserted-by":"publisher","first-page":"14986","DOI":"10.1074\/jbc.M108303200","article-title":"A nucleotide switch in the Escherichia coli DnaA protein initiates chromosomal replication: Evidnece from a mutant DnaA protein defective in regulatory ATP hydrolysis in vitro and in vivo.","volume":"277","author":"Nishida","year":"2002","journal-title":"J. Biol. Chem."},{"key":"B41","doi-asserted-by":"publisher","first-page":"20295","DOI":"10.1074\/jbc.M115.662601","article-title":"The Arg fingers of key DnaA protomers are oriented inward within the replication origin oriC and stimulate DnaA subcomplexes in the initiation complex.","volume":"290","author":"Noguchi","year":"2015","journal-title":"J. Biol. Chem."},{"key":"B42","doi-asserted-by":"publisher","first-page":"3379","DOI":"10.1099\/mic.0.2008\/019745-0","article-title":"Determination of the minimum domain II size of Escherichia coli DnaA protein essential for cell viability.","volume":"154","author":"Nozaki","year":"2008","journal-title":"Microbiology"},{"key":"B43","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1111\/j.1365-2443.2008.01269.x","article-title":"Initiator titration complex formed at datA with the aid of IHF regulates replication timing in Escherichia coli.","volume":"14","author":"Nozaki","year":"2009","journal-title":"Genes Cells"},{"key":"B44","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1093\/nar\/gkr716","article-title":"Regulation of chromosomal replication initiation by oriC-proximal DnaA-box clusters in Bacillus subtilis.","volume":"40","author":"Okumura","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"B45","doi-asserted-by":"publisher","first-page":"653","DOI":"10.1111\/j.1432-1033.1991.tb16326.x","article-title":"Analysis of the complex transcription termination region of the Escherichia coli rrnB gene.","volume":"201","author":"Orosz","year":"1991","journal-title":"Eur. J. Biochem."},{"key":"B46","doi-asserted-by":"publisher","first-page":"8351","DOI":"10.1074\/jbc.M708684200","article-title":"A common mechanism for the ATP-DnaA-dependent formation of open complexes at the replication origin.","volume":"283","author":"Ozaki","year":"2008","journal-title":"J. Biol. Chem."},{"key":"B47","doi-asserted-by":"crossref","first-page":"1985","DOI":"10.1016\/S0021-9258(18)52389-9","article-title":"Integration host factor-mediated expression of the ilvGMEDA operon of Escherichia coli.","volume":"266","author":"Pagel","year":"1991","journal-title":"J. Biol. Chem."},{"key":"B48","doi-asserted-by":"publisher","first-page":"3524","DOI":"10.1093\/nar\/gkr1236","article-title":"Genomic analysis of DNA binding and gene regulation by homologous nucleoid-associated proteins IHF and HU in Escherichia coli K12.","volume":"40","author":"Prieto","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"B49","doi-asserted-by":"publisher","first-page":"12354","DOI":"10.1093\/nar\/gkx914","article-title":"Regulatory dynamics in the ternary DnaA complex for initiation of chromosomal replication in Escherichia coli.","volume":"45","author":"Sakiyama","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"B50","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1016\/0378-1119(88)90499-4","article-title":"Termination of the Escherichia coli asnC transcript. The DnaA protein\/dnaA box complex blocks transcribing RNA polymerase.","volume":"73","author":"Schaefer","year":"1988","journal-title":"Gene"},{"key":"B51","doi-asserted-by":"publisher","first-page":"1609","DOI":"10.1002\/j.1460-2075.1989.tb03545.x","article-title":"Directionality of DnaA protein\/DNA interaction. Active orientation of the DnaA protein\/dnaA box complex in transcription termination.","volume":"8","author":"Schaefer","year":"1989","journal-title":"EMBO J."},{"key":"B52","doi-asserted-by":"publisher","first-page":"E8021","DOI":"10.1073\/pnas.1609649113","article-title":"Near-atomic structural model for bacterial DNA replication initiation complex and its functional insights.","volume":"113","author":"Shimizu","year":"2016","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B53","doi-asserted-by":"publisher","first-page":"6184","DOI":"10.1128\/JB.00528-06","article-title":"Cluster of DnaA boxes involved in regulation of Streptomyces chromosome replication: From in silico to in vivo studies.","volume":"188","author":"Smulczyk-Krawczyszyn","year":"2006","journal-title":"J. Bacteriol."},{"key":"B54","doi-asserted-by":"publisher","first-page":"731","DOI":"10.1046\/j.1365-2443.2003.00671.x","article-title":"Transcriptional control for initiation of chromosomal replication in Escherichia coli: Fluctuation of the level of origin transcription ensures timely initiation.","volume":"8","author":"Su\u2019etsugu","year":"2003","journal-title":"Genes Cells"},{"key":"B55","doi-asserted-by":"publisher","first-page":"11209","DOI":"10.1093\/nar\/gkz795","article-title":"A novel mode of DnaA-DnaA interaction promotes ADP dissociation for reactivation of replication initiation activity.","volume":"47","author":"Sugiyama","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"B56","doi-asserted-by":"publisher","first-page":"34255","DOI":"10.1074\/jbc.273.51.34255","article-title":"Escherichia coli DnaA protein. The N-terminal domain and loading of DnaB helicase at the E. coli chromosomal origin.","volume":"273","author":"Sutton","year":"1998","journal-title":"J. Biol. Chem."},{"key":"B57","doi-asserted-by":"publisher","first-page":"575","DOI":"10.1111\/j.1365-2958.1993.tb00929.x","article-title":"Correlation of gene transcription with the time of initiation of chromosome replication in Escherichia coli.","volume":"10","author":"Theisen","year":"1993","journal-title":"Mol. Microbiol."},{"key":"B58","doi-asserted-by":"publisher","first-page":"5264","DOI":"10.1016\/j.csbj.2022.09.020","article-title":"Structural interplay between DNA-shape protein recognition and supercoiling: The case of IHF.","volume":"20","author":"Watson","year":"2022","journal-title":"Comput. Struct. Biotechnol. J."},{"key":"B59","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1261\/rna.064436.117","article-title":"Examining tRNA 3\u2019-ends in Escherichia coli: Teamwork between CCA-adding enzyme, RNase T, and RNase R.","volume":"24","author":"Wellner","year":"2018","journal-title":"RNA"},{"key":"B60","doi-asserted-by":"crossref","first-page":"1624","DOI":"10.1128\/JB.180.7.1624-1631.1998","article-title":"Characterization of dnaC2 and dnaC28 mutants by flow cytometry.","volume":"180","author":"Withers","year":"1998","journal-title":"J. Bacteriol."},{"key":"B61","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1002\/mlf2.12075","article-title":"A DnaA-dependent riboswitch for transcription attenuation of the his operon.","volume":"2","author":"Yao","year":"2023","journal-title":"mLife"},{"key":"B62","doi-asserted-by":"publisher","first-page":"6286","DOI":"10.1093\/nar\/gkad389","article-title":"Single-stranded DNA recruitment mechanism in replication origin unwinding by DnaA initiator protein and HU, an evolutionary ubiquitous nucleoid protein.","volume":"51","author":"Yoshida","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.3390\/genes8050136","article-title":"The role of the N-Terminal domains of bacterial initiator DnaA in the assembly and regulation of the bacterial replication initiation complex.","volume":"8","author":"Zawilak-Pawlik","year":"2017","journal-title":"Genes (Basel)"}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2024.1360108\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,5]],"date-time":"2024-03-05T16:52:26Z","timestamp":1709657546000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2024.1360108\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,28]]},"references-count":63,"alternative-id":["10.3389\/fmicb.2024.1360108"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2024.1360108","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,28]]},"article-number":"1360108"}}