{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T02:49:42Z","timestamp":1791600582160,"version":"4.3.4"},"reference-count":115,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T00:00:00Z","timestamp":1715558400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>In recent years, the world\u2019s attention has been drawn to antimicrobial resistance (AMR) because to the frightening prospect of growing death rates. Nanomaterials are being investigated due to their potential in a wide range of technical and biological applications.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The purpose of this study was to biosynthesis zinc oxide nanoparticles (ZnONPs) using <jats:italic>Aspergillus<\/jats:italic> sp. SA17 fungal extract, followed by characterization of the produced nanoparticles (NP) using electron microscopy (TEM and SEM), UV-analysis, X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FT-IR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results and Discussion<\/jats:title><jats:p>The HR-TEM revealed spherical nanoparticles with an average size of 7.2 nm, and XRD validated the crystalline nature and crystal structure features of the generated ZnONPs, while the zeta potential was 18.16 mV, indicating that the particles\u2019 surfaces are positively charged. The FT-IR was also used to identify the biomolecules involved in the synthesis of ZnONPs. The antibacterial and anticancer properties of both the crude fungal extract and its nano-form against several microbial strains and cancer cell lines were also investigated. Inhibition zone diameters against pathogenic bacteria ranged from 3 to 13 mm, while IC<jats:sub>50<\/jats:sub> values against cancer cell lines ranged from 17.65 to 84.55 M. Additionally, 33 compounds, including flavonoids, phenolic acids, coumarins, organic acids, anthraquinones, and lignans, were discovered through chemical profiling of the extract using UPLC-QTOF-MS\/MS. Some molecules, such pomiferin and glabrol, may be useful for antibacterial purposes, according to <jats:italic>in silico<\/jats:italic> study, while daidzein 4\u2019-sulfate showed promise as an anti-cancer metabolite.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2024.1366614","type":"journal-article","created":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T04:34:16Z","timestamp":1715574856000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":23,"title":["Bio-fabricated zinc oxide nanoparticles mediated by endophytic fungus Aspergillus sp. SA17 with antimicrobial and anticancer activities: in vitro supported by in silico studies"],"prefix":"10.3389","volume":"15","author":[{"given":"Sally El Said Abo Halawa","family":"Abdelrahman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seham","family":"El Hawary","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Engy","family":"Mohsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed A.","family":"El Raey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heba Mohammed Refat M.","family":"Selim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed M. E.","family":"Hamdan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mosad A.","family":"Ghareeb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed A.","family":"Hamed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,5,13]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1556\/1326.2017.00329","article-title":"Chromatographic isolation and structural elucidation of secondary metabolites from the soil-inhabiting fungus Aspergillus fumigatus 3T-EGY","volume":"30","author":"Abdel-Aziz","year":"2018","journal-title":"Acta Chromatogr."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"1057","DOI":"10.21608\/EJCHEM.2020.43379.2878","article-title":"Biosynthesis and bio-sensing applications of silver and gold metal nanoparticles","volume":"64","author":"Abdelaziz","year":"2021","journal-title":"Egypt. J. Chem."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"1444","DOI":"10.3390\/antiox11081444","article-title":"Green synthesis and characterization of ZnO nanoparticles using Pelargonium odoratissimum (L.) aqueous leaf extract and their antioxidant, antibacterial and anti-inflammatory activities","volume":"11","author":"Abdelbaky","year":"2022","journal-title":"Antioxidants"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"2813","DOI":"10.3390\/plants12152813","article-title":"Phytochemical profiling and antiviral activity of green sustainable nanoparticles derived from Maesa indica (Roxb.) sweet against human coronavirus 229E","volume":"12","author":"Abdelgawad","year":"2023","journal-title":"Plan. Theory"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"1704","DOI":"10.3390\/molecules27051704","article-title":"The chemical profiling, docking study, and antimicrobial and antibiofilm activities of the endophytic fungi aspergillus sp. AP5","volume":"27","author":"Abdelgawad","year":"2022","journal-title":"Molecules"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/j.culher.2021.11.004","article-title":"Evaluation of the efficiency of nanoparticles for increasing \u03b1-amylase enzyme activity for removing starch stain from paper artifacts","volume":"53","author":"Abdel-Nasser","year":"2022","journal-title":"J. Cult. Herit."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"101726","DOI":"10.1016\/j.bcab.2020.101726","article-title":"Production and purification of bioactive compounds with potent antimicrobial activity from a novel terrestrial fungus aspergillus sp. DHE. Biocatal","volume":"28","author":"Abdel-Razek","year":"2020","journal-title":"Agric. Biotechnol."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"265","DOI":"10.17582\/journal.aavs\/2021\/9.2.265.273","article-title":"Anticancer and antioxidant activity by secondary metabolites of Aspergillus fumigatus","volume":"9","author":"Almanaa","year":"2021","journal-title":"Adv. Anim. Vet. Sci."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"266","DOI":"10.3390\/molecules28010266","article-title":"Green synthesized zinc oxide nanoparticles based on Cestrum diurnum L. of potential antiviral activity against human Corona 229-E virus","volume":"28","author":"Alrabayah","year":"2022","journal-title":"Molecules"},{"key":"ref10","doi-asserted-by":"publisher","first-page":"616","DOI":"10.1002\/pca.2928","article-title":"Untargeted metabolite profiling and phytochemical analysis based on RP-HPLC-DAD-QTOF-MS and MS\/MS for discovering new bioactive compounds in Rumex algeriensis flowers and stems","volume":"31","author":"Ammar","year":"2020","journal-title":"Phytochem. Anal."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"691","DOI":"10.1038\/nchembio.117","article-title":"Targeted polypharmacology: discovery of dual inhibitors of tyrosine and phosphoinositide kinases","volume":"4","author":"Apsel","year":"2008","journal-title":"Nat. Chem. Biol."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"111724","DOI":"10.1016\/j.colsurfb.2021.111724","article-title":"Antiviral zinc oxide nanoparticles mediated by hesperidin and in silico comparison study between antiviral phenolics as anti-SARS-CoV-2","volume":"203","author":"Attia","year":"2021","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1016\/B978-0-12-821938-6.00022-0","article-title":"Algae-, fungi-, and yeast-mediated biological synthesis of nanoparticles and their various biomedical applications","volume-title":"Handbook of greener synthesis of nanomaterials and compounds","author":"Bachheti","year":"2021"},{"key":"ref14","doi-asserted-by":"publisher","first-page":"570","DOI":"10.1016\/j.bjp.2019.05.005","article-title":"RP-HPLC and LC-MS-MS determination of a bioactive artefact from Ipomoea pescaprae extract","volume":"29","author":"Barth","year":"2019","journal-title":"Rev. Bras"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"827","DOI":"10.1071\/CH13639","article-title":"Fungal metabolites as pharmaceuticals","volume":"67","author":"Beekman","year":"2014","journal-title":"Aust. J. Chem."},{"key":"ref16","doi-asserted-by":"publisher","first-page":"97","DOI":"10.3390\/md21020097","article-title":"Bioprospecting of targeted phenolic compounds of Dictyota dichotoma, Cystoseira barbata, Ericaria amentacea, Sargassum hornschuchii and Ellisolandia elongata from the Adriatic Sea extracted by two green methods","volume":"21","author":"Castillo","year":"2023","journal-title":"Mar. Drugs"},{"key":"ref17","doi-asserted-by":"publisher","first-page":"101320","DOI":"10.1016\/j.mtcomm.2020.101320","article-title":"Characterization, antioxidant, antimicrobial and cytotoxic activities of green synthesized silver and iron nanoparticles using alcoholic Blumea eriantha DC plant extract","volume":"24","author":"Chavan","year":"2020","journal-title":"Mater. Today Commun."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"316","DOI":"10.1016\/j.jpba.2015.12.051","article-title":"Determination of pinostilbene in rat plasma by LC-MS\/MS: application to a pharmacokinetic study","volume":"120","author":"Chen","year":"2016","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"3909","DOI":"10.1016\/j.jchromb.2011.11.002","article-title":"LC-MS\/MS-based metabolites of Eurycoma longifolia (Tongkat Ali) in Malaysia (Perak and Pahang)","volume":"879","author":"Chua","year":"2011","journal-title":"J. Chromatogr. B"},{"key":"ref20","author":"Clugston","year":"1996"},{"key":"ref21","doi-asserted-by":"publisher","first-page":"79","DOI":"10.24425\/abcsb.2020.131666","article-title":"Characterization of flavonoid components in Scutellaria L. species (Lamiaceae) using fingerprinting analysis","volume":"62","author":"Dehkordi","year":"2020","journal-title":"Acta Biol. Cracov. Bot."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"1062","DOI":"10.3390\/microorganisms11041062","article-title":"Bioactive metabolite from endophytic aspergillus versicolor SB5 with anti-acetylcholinesterase, anti-inflammatory and antioxidant activities: in vitro and in silico studies","volume":"11","author":"Elawady","year":"2023","journal-title":"Microorganisms."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"926","DOI":"10.1080\/10826068.2021.1875236","article-title":"Potential of silver nanoparticles synthesized using low active mosquitocidal Lysinibacillus sphaericus as novel antimicrobial agents","volume":"51","author":"El-Bendary","year":"2021","journal-title":"Prep. Biochem. Biotechnol."},{"key":"ref24","doi-asserted-by":"publisher","first-page":"434","DOI":"10.5530\/pj.2021.13.55","article-title":"Bioactive secondary metabolite from endophytic aspergillus tubenginses ASH4 isolated from Hyoscyamus muticus: antimicrobial, antibiofilm, antioxidant and anticancer activity","volume":"13","author":"Elkhouly","year":"","journal-title":"Pharmacogenomics J."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"7511","DOI":"10.21608\/EJCHEM.2021.85908.4161","article-title":"Bioactive secondary metabolites from endophytic aspergillus terreus AH1 isolated from Ipomoea carnea growing in Egypt","author":"Elkhouly","year":"","journal-title":"Egypt. J. 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J."},{"key":"ref29","doi-asserted-by":"publisher","first-page":"4678","DOI":"10.1039\/C5AY00993F","article-title":"A practical method for the rapid detection and structural characterization of major constituents from traditional Chinese medical formulas: analysis multiple constituents in Yinchenhao decoction","volume":"7","author":"Fu","year":"2015","journal-title":"Anal. Methods"},{"key":"ref30","doi-asserted-by":"publisher","first-page":"827","DOI":"10.1002\/mnfr.200500091","article-title":"Broad spectrum antiinfective potential of xanthohumol from hop (Humulus lupulus L.) in comparison with activities of other hop constituents and xanthohumol metabolites","volume":"49","author":"Gerh\u00e4user","year":"2005","journal-title":"Mol. Nutr. Food Res."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"162","DOI":"10.4103\/pr.pr_102_18","article-title":"Bioactive secondary metabolites from the locally isolated terrestrial fungus Penicillium sp. SAM16-EGY","volume":"11","author":"Ghareeb","year":"","journal-title":"Pharm. Res."},{"key":"ref32","doi-asserted-by":"publisher","first-page":"308","DOI":"10.2174\/1573407214666181009110805","article-title":"Chemical profiles and bio-activities of different extracts of Terfezia species and their other associated fungi","volume":"16","author":"Ghareeb","year":"2020","journal-title":"Curr. Bioact. Compd."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1111\/jphp.12843","article-title":"HPLC-DAD-ESI-MS\/MS analysis of fruits from Firmiana simplex (L.) and evaluation of their antioxidant and antigenotoxic properties","volume":"70","author":"Ghareeb","year":"","journal-title":"J. Pharm. Pharmacol."},{"key":"ref34","doi-asserted-by":"publisher","first-page":"3469","DOI":"10.1038\/s41598-024-53336-1","article-title":"Ipomoea carnea mitigates ethanol-induced ulcers in irradiated rats via Nrf2\/HO-1 pathway: an in vivo and in silico study","volume":"14","author":"Ghareeb","year":"2024","journal-title":"Sci. Rep."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"415","DOI":"10.3390\/pharmaceutics15020415","article-title":"Polyphenolic profile of Herniaria hemistemon aerial parts extract and assessment of its anti-cryptosporidiosis in a murine model: in silico supported in vivo study","volume":"15","author":"Ghareeb","year":"2023","journal-title":"Pharmaceutics."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"4153","DOI":"10.3390\/antiox8090415","article-title":"Chemical profiling of polyphenolics in Eucalyptus globulus and evaluation of its hepato-renal protective potential against cyclophosphamide induced toxicity in mice","volume":"8","author":"Ghareeb","year":"","journal-title":"Antioxidants."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"3238","DOI":"10.3390\/molecules23123238","article-title":"HPLC-ESI-MS\/MS profiling of polyphenolics of a leaf extract from Alpinia zerumbet (Zingiberaceae) and its anti-inflammatory, anti-nociceptive, and antipyretic activities in vivo","volume":"23","author":"Ghareeb","year":"","journal-title":"Molecules"},{"key":"ref38","doi-asserted-by":"publisher","first-page":"817","DOI":"10.1002\/oms.1210240916","article-title":"Studies of phthalic acid and related compounds by positive- and negative-ion chemical ionization tandem mass spectrometry (\u00b1 CI\/MS\/MS)","volume":"24","author":"Gillespie","year":"1989","journal-title":"Mass Spectrom."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"4114","DOI":"10.1007\/s10904-022-02406-w","article-title":"Microbial mediated synthesis of zinc oxide nanoparticles, characterization and multifaceted applications","volume":"32","author":"Gomaa","year":"2022","journal-title":"J. Inorg. Organomet. Polym. Mater."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"465","DOI":"10.3390\/ijms20030465","article-title":"Vanillic acid suppresses HIF-1\u03b1 expression via inhibition of mTOR\/p70S6K\/4E-BP1 and Raf\/MEK\/ERK pathways in human colon cancer HCT116 cells","volume":"20","author":"Gong","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"e2400092","DOI":"10.1002\/ardp.202400092","article-title":"Health benefits of fraxetin: from chemistry to medicine","author":"Ha","year":"2024","journal-title":"Arch. Pharm."},{"key":"ref42","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1186\/s42269-018-0027-0","article-title":"Antimicrobial and antioxidant activities of different extracts from aspergillus unguis SPMD-EGY grown on different media","volume":"42","author":"Hamed","year":"2018","journal-title":"Bull. Natl. Res. Cent."},{"key":"ref43","doi-asserted-by":"publisher","first-page":"1617","DOI":"10.3390\/microorganisms8101617","article-title":"Screening fungal endophytes derived from under explored Egyptian marine habitats for antimicrobial and antioxidant properties in factionalised textiles","volume":"8","author":"Hamed","year":"2020","journal-title":"Microorganisms."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"928","DOI":"10.3390\/mi14050928","article-title":"Green synthesis of zinc oxide (ZnO) nanoparticles from green algae and their assessment in various biological applications","volume":"14","author":"Hameed","year":"2023","journal-title":"Micromachines."},{"key":"ref45","author":"Hanley","year":"2006"},{"key":"ref46","doi-asserted-by":"crossref","DOI":"10.1007\/978-94-017-9780-1_22","article-title":"Optical properties of metal nanoparticles","volume-title":"Encyclopedia of nanotechnology","author":"Harris","year":"2016"},{"key":"ref47","first-page":"1000","volume-title":"Using AutoDock 4 and AutoDock vina with AutoDockTools: a tutorial","author":"Huey","year":"2012"},{"key":"ref48","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/0263-7855(96)00018-5","article-title":"VMD: visual molecular dynamics","volume":"14","author":"Humphrey","year":"1996","journal-title":"J. Mol. Graph."},{"key":"ref49","doi-asserted-by":"publisher","first-page":"1117","DOI":"10.3390\/molecules27031117","article-title":"Identification of antibacterial metabolites from endophytic fungus Aspergillus fumigatus, isolated from Albizia lucidior leaves (Fabaceae), utilizing metabolomic and molecular docking techniques","volume":"27","author":"Hussein","year":"2022","journal-title":"Molecules"},{"key":"ref50","doi-asserted-by":"publisher","first-page":"20988","DOI":"10.1038\/s41598-021-99839-z","article-title":"Green synthesis of zinc oxide nanoparticles using Elaeagnus angustifolia L. leaf extracts and their multiple in vitro biological applications","volume":"11","author":"Iqbal","year":"2021","journal-title":"Sci. Rep."},{"key":"ref51","year":"2015"},{"key":"ref52","doi-asserted-by":"publisher","first-page":"3107","DOI":"10.3390\/nu13093107","article-title":"Plant-derived and dietary hydroxybenzoic acids-a comprehensive study of structural, anti\u2212\/pro-oxidant, lipophilic, antimicrobial, and cytotoxic activity in MDA-MB-231 and MCF-7 cell lines","volume":"13","author":"Kalinowska","year":"2021","journal-title":"Nutrients"},{"key":"ref53","doi-asserted-by":"publisher","first-page":"114335","DOI":"10.1016\/j.jpba.2021.114335","article-title":"Development and validation of a LC-MS\/MS method for quantitation of 3-hydroxypentanoic acid and 3-oxopentanoic acid in human plasma and its application to a clinical study of glucose transporter type I deficiency (G1D) syndrome","volume":"205","author":"Kallem","year":"2021","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref54","doi-asserted-by":"publisher","first-page":"e202104406","DOI":"10.1002\/slct.202104406","article-title":"UHPLC-ESI-QTOF-MS\/MS characterisation of phenolic compounds from Tithonia diversifolia (Hemsl.) a Gray and antioxidant activity","volume":"7","author":"Kerebba","year":"2022","journal-title":"ChemistrySelect."},{"key":"ref55","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1556\/1326.2018.00412","article-title":"Phenolic constituents, antimicrobial, antioxidant and anticancer activities of ethyl acetate and n-butanol extracts of Senna italica","volume":"31","author":"Khalaf","year":"2019","journal-title":"Acta Chromatogr."},{"key":"ref56","doi-asserted-by":"publisher","first-page":"1433","DOI":"10.1080\/14756366.2020.1786819","article-title":"LC-QTOF-MS analysis of xanthone content in different parts of Garcinia mangostana and its influence on cholinesterase inhibition","volume":"35","author":"Khaw","year":"2020","journal-title":"J. Enzyme Inhib. Med. Chem."},{"key":"ref57","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1186\/s13568-023-01568-1","article-title":"Antimicrobial, antiproliferative activities and molecular docking of metabolites from Alternaria alternata","volume":"13","author":"Khazaal","year":"2023","journal-title":"AMB Express"},{"key":"ref58","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1016\/j.jpha.2017.01.005","article-title":"Identification and characterization of phenolics and terpenoids from ethanolic extracts of Phyllanthus species by HPLC-ESI-QTOF-MS\/MS","volume":"7","author":"Kumar","year":"2017","journal-title":"J. Pharm. Anal."},{"key":"ref59","doi-asserted-by":"publisher","first-page":"1019","DOI":"10.1111\/j.1365-2672.2009.04285.x","article-title":"Aspergillus fumigatus Fresenius, an endophytic fungus from Juniperus communis L. Horstmann as a novel source of the anticancer pro-drug deoxypodophyllotoxin","volume":"107","author":"Kusari","year":"2009","journal-title":"J. Appl. Microbiol."},{"key":"ref60","doi-asserted-by":"publisher","first-page":"930","DOI":"10.3390\/cells8080930","article-title":"EGFR-c-Src-mediated HDAC3 phosphorylation exacerbates invasion of breast cancer cells","volume":"8","author":"Kwak","year":"2019","journal-title":"Cells"},{"key":"ref61","doi-asserted-by":"publisher","first-page":"10552","DOI":"10.1039\/C9RA10925K","article-title":"The development and validation of an HPLC-MS\/MS method for the determination of eriocitrin in rat plasma and its application to a pharmacokinetic study","volume":"10","author":"Li","year":"2020","journal-title":"RSC Adv."},{"key":"ref62","doi-asserted-by":"publisher","first-page":"277","DOI":"10.3390\/md21050277","article-title":"Marine aspergillus: a treasure trove of antimicrobial compounds","volume":"21","author":"Li","year":"2023","journal-title":"Mar. Drugs"},{"key":"ref63","doi-asserted-by":"publisher","first-page":"127","DOI":"10.4028\/www.scientific.net\/AMR.716.127","article-title":"Surface charge research of graphene oxide, chemically reduced graphene oxide and thermally exfoliated graphene oxide","volume":"716","author":"Li","year":"2013","journal-title":"Adv. Mat. Res."},{"key":"ref64","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1016\/j.micres.2007.07.002","article-title":"Diversity of fungal isolates from three Hawaiian marine sponges","volume":"164","author":"Li","year":"2009","journal-title":"Microbiol. Res."},{"key":"ref65","doi-asserted-by":"publisher","first-page":"1266","DOI":"10.3390\/molecules21101266","article-title":"Identification of metabolites of 6'-Hydroxy-3,4,5,2\u2032,4\u2032-pentamethoxychalcone in rats by a combination of ultra-high-performance liquid chromatography with linear ion trap-orbitrap mass spectrometry based on multiple data processing techniques","volume":"21","author":"Liu","year":"2016","journal-title":"Molecules"},{"key":"ref66","doi-asserted-by":"publisher","first-page":"023","DOI":"10.7324\/JAPS.2017.70604","article-title":"Gas chromatography-mass spectrometry analysis, antimicrobial, anticancer and antioxidant activities of n-hexane and methylene chloride extracts from Senna italica","volume":"7","author":"Madkour","year":"2017","journal-title":"J. Appl. Pharm. Sci."},{"key":"ref67","doi-asserted-by":"publisher","first-page":"95","DOI":"10.3390\/md21020095","article-title":"Bioactivity and metabolome mining of deep-sea sediment-derived microorganisms reveal new hybrid PKS-NRPS macrolactone from aspergillus versicolor PS108-62","volume":"21","author":"Magot","year":"2023","journal-title":"Mar. Drugs"},{"key":"ref68","doi-asserted-by":"publisher","first-page":"2175","DOI":"10.21608\/EJCHEM.2023.209102.7935","article-title":"UPLC-QTOF\/MS-assisted chemical profiling of Daucus carota leaf extract and evaluation of its antioxidant, antimicrobial and antibiofilm activities: evidence from in vitro and in silico studies","volume":"66","author":"Mahmoud","year":"2023","journal-title":"Egypt. J. Chem."},{"key":"ref69","doi-asserted-by":"publisher","first-page":"1581","DOI":"10.1002\/jms.1480","article-title":"Analysis of flavonoids: tandem mass spectrometry, computational methods, and NMR","volume":"43","author":"March","year":"2008","journal-title":"J. Mass Spectrom."},{"key":"ref70","first-page":"532","volume-title":"Handbook of hydroxyacetophenones","author":"Martin","year":"2013"},{"key":"ref71","doi-asserted-by":"publisher","first-page":"224","DOI":"10.1016\/j.micpath.2018.05.041","article-title":"Biologically synthesized zinc oxide nanoparticles as nanoantibiotics against ESBLs producing gram negative bacteria","volume":"121","author":"Maruthupandy","year":"2018","journal-title":"Microb. Pathog."},{"key":"ref72","doi-asserted-by":"publisher","first-page":"7361","DOI":"10.1073\/pnas.0811578106","article-title":"Ancient Egyptian herbal wines","volume":"106","author":"McGovern","year":"2009","journal-title":"Proc. Natl. Acad. Sci."},{"key":"ref73","doi-asserted-by":"publisher","first-page":"3314","DOI":"10.1021\/ct300418h","article-title":"MMPBSA. Py: an efficient program for end-state free energy calculations","volume":"8","author":"Miller","year":"2012","journal-title":"J. Chem. Theory Comput."},{"key":"ref74","doi-asserted-by":"publisher","first-page":"173","DOI":"10.3390\/ijms22010173","article-title":"PI3K\/AKT\/mTOR signaling pathway in breast cancer: from molecular landscape to clinical aspects","volume":"22","author":"Miricescu","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"ref75","doi-asserted-by":"publisher","first-page":"1800","DOI":"10.3390\/nano13111800","article-title":"Size matters? A comprehensive in vitro study of the impact of particle size on the toxicity of ZnO","volume":"13","author":"Mitjans","year":"2023","journal-title":"Nanomaterials"},{"key":"ref76","doi-asserted-by":"publisher","first-page":"e2200360","DOI":"10.1002\/ardp.202200360","article-title":"Mechanistic study of the antibacterial potential of the prenylated flavonoid auriculasin against Escherichia coli","volume":"355","author":"Mohamed","year":"2022","journal-title":"Arch. Pharm."},{"key":"ref77","doi-asserted-by":"publisher","first-page":"1875","DOI":"10.3390\/life12111875","article-title":"Garcixanthone E and Garcimangophenone C: new metabolites from Garcinia mangostana and their cytotoxic and alpha amylase inhibitory potential","volume":"12","author":"Mohamed","year":"2022","journal-title":"Life."},{"key":"ref78","doi-asserted-by":"publisher","first-page":"280","DOI":"10.1002\/jms.3339","article-title":"Neutral and acidic products derived from hydroxyl radical-induced oxidation of arabinotriose assessed by electrospray ionisation mass spectrometry","volume":"49","author":"Moreira","year":"2014","journal-title":"J. Mass Spectrom."},{"key":"ref79","doi-asserted-by":"publisher","first-page":"100766","DOI":"10.1016\/j.mtcomm.2019.100766","article-title":"Biologically synthesized copper and zinc oxide nanoparticles for important biomolecules detection and antimicrobial applications","volume":"22","author":"Muthuchamy","year":"2020","journal-title":"Mater. Today Commun."},{"key":"ref80","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/2191-2858-2-9","article-title":"Four butyrolactones and diverse bioactive secondary metabolites from terrestrial aspergillus flavipes MM2: isolation and structure determination","volume":"2","author":"Nagia","year":"2012","journal-title":"Org. Med. Chem. Lett."},{"key":"ref81","doi-asserted-by":"publisher","first-page":"436","DOI":"10.3390\/jof7060436","article-title":"Potential of anti-cancer activity of secondary metabolic products from marine fungi","volume":"7","author":"Noman","year":"2021","journal-title":"J. Fungi."},{"key":"ref82","doi-asserted-by":"publisher","first-page":"7629","DOI":"10.1371\/journal.pone.0097629","article-title":"Comparison and validation of some ITS primer pairs useful for fungal metabarcoding studies","volume":"9","author":"Op De Beeck","year":"2014","journal-title":"PLoS One"},{"key":"ref83","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1016\/j.apcatb.2017.03.005","article-title":"Photocatalysts fabricated by depositing plasmonic ag nanoparticles on carbon quantum dots\/graphitic carbon nitride for broad spectrum photocatalytic hydrogen generation","volume":"209","author":"Qin","year":"2017","journal-title":"Appl. Catal. Environ."},{"key":"ref84","doi-asserted-by":"publisher","first-page":"190","DOI":"10.1016\/j.micpath.2018.03.011","article-title":"Antibiofilm effect of Nocardiopsis sp. GRG 1 (KT235640) compound against biofilm forming gram negative bacteria on UTIs","volume":"118","author":"Rajivgandhi","year":"2018","journal-title":"Microb. Pathog."},{"key":"ref85","doi-asserted-by":"publisher","first-page":"4894","DOI":"10.1557\/s43578-021-00431-y","article-title":"Colon targeted dosage form of Capecitabine using folic acid anchored modified carbon nanotube: in vitro cytotoxicity, apoptosis and in vivo roentgen graphic study","volume":"36","author":"Randive","year":"2021","journal-title":"Drug Dev. Ind. Pharm."},{"key":"ref86","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1186\/s43094-023-00543-8","article-title":"Efficient in vitro oxaliplatin delivery with functionalized single-walled carbon nanotube for enhanced colon cancer treatment","volume":"9","author":"Randive","year":"2023","journal-title":"Fut J. Pharm. Sci."},{"key":"ref87","doi-asserted-by":"publisher","first-page":"61","DOI":"10.53879\/id.57.08.12514","article-title":"Green synthesis of gold nanoparticles of isolated Citrus bioflavonoid from orange: characterization and in vitro cytotoxicity against colon cancer cell lines COLO 320DM and HT29","volume":"57","author":"Randive","year":"2020","journal-title":"Indian Drugs"},{"key":"ref88","author":"Reed","year":"2009"},{"key":"ref89","doi-asserted-by":"publisher","first-page":"673a","DOI":"10.1016\/j.bpj.2017.11.3632","article-title":"QwikMD-gateway for easy simulation with VMD and NAMD","volume":"114","author":"Ribeiro","year":"2018","journal-title":"Biophys. J."},{"key":"ref90","doi-asserted-by":"publisher","first-page":"4010018","DOI":"10.1155\/2022\/4010018","article-title":"Antimicrobial activity of aspergillus sp. from the Amazon biome: isolation of Kojic acid","volume":"2022","author":"Rodrigues","year":"2022","journal-title":"Int. J. Microbiol."},{"key":"ref91","doi-asserted-by":"publisher","first-page":"67","DOI":"10.5897\/JPP2017.0452","article-title":"Chemical constituents and biological activities of different solvent extracts of Prosopis farcta growing in Egypt","volume":"9","author":"Saad","year":"2017","journal-title":"J. Pharmacogn. Phytother."},{"key":"ref92","doi-asserted-by":"publisher","first-page":"11733","DOI":"10.1039\/D2FO01915A","article-title":"Antiproliferative potential of Physalis peruviana-derived magnolin against pancreatic cancer: a comprehensive in vitro and in silico study","volume":"13","author":"Sayed","year":"2022","journal-title":"Food Funct."},{"key":"ref93","doi-asserted-by":"publisher","first-page":"659","DOI":"10.1107\/S1600576714003379","article-title":"Simultaneous determination of several crystal structures from powder mixtures: the combination of powder X-ray diffraction, band-target entropy minimization and Rietveld methods","volume":"47","author":"Schreyer","year":"2014","journal-title":"J. Appl. Cryst."},{"key":"ref94","doi-asserted-by":"publisher","first-page":"4032820","DOI":"10.1155\/2017\/4032820","article-title":"Rapid screening of chemical constituents in Rhizoma anemarrhenae by UPLC-Q-TOF\/MS combined with data post processing techniques","volume":"2017","author":"Shan","year":"2017","journal-title":"Evid. Based Complement. Alternat. Med."},{"key":"ref95","doi-asserted-by":"publisher","first-page":"3083","DOI":"10.1002\/rcm.4215","article-title":"A rapid screening method for prenylated flavonoids with ultra-high-performance liquid chromatography\/electrospray ionisation mass spectrometry in licorice root extracts","volume":"23","author":"Simons","year":"2009","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"ref96","doi-asserted-by":"publisher","first-page":"1573","DOI":"10.3390\/biom13111573","article-title":"Norlobaridone inhibits quorum sensing-dependent biofilm formation and some virulence factors in Pseudomonas aeruginosa by disrupting its transcriptional activator protein LasR dimerization","volume":"13","author":"Soltane","year":"2023","journal-title":"Biomol. Ther."},{"key":"ref97","doi-asserted-by":"publisher","first-page":"180167","DOI":"10.1038\/sdata.2018.167","article-title":"Comprehensive high-resolution multiple-reaction monitoring mass spectrometry for targeted eicosanoid assays","volume":"5","author":"Sorgi","year":"2018","journal-title":"Sci. Data."},{"key":"ref98","doi-asserted-by":"publisher","first-page":"608","DOI":"10.3390\/molecules21050608","article-title":"Antimicrobial activity of xanthohumol and its selected structural analogues","volume":"21","author":"Stompor","year":"2016","journal-title":"Molecules"},{"key":"ref99","doi-asserted-by":"publisher","first-page":"e8978","DOI":"10.7717\/peerj.8978","article-title":"Isolation and identification of flavonoid-producing endophytic fungi from medicinal plant Conyza blinii H.L\u00e9v that exhibit higher antioxidant and antibacterial activities","volume":"8","author":"Tang","year":"2022","journal-title":"PeerJ."},{"key":"ref100","doi-asserted-by":"publisher","first-page":"6915","DOI":"10.1016\/j.febslet.2006.11.056","article-title":"Phenylpropanoid glycosyltransferases from Osage orange (Maclura pomifera) fruit","volume":"580","author":"Tian","year":"2006","journal-title":"FEBS Lett."},{"key":"ref101","doi-asserted-by":"publisher","first-page":"10145","DOI":"10.1021\/acsomega.0c00865","article-title":"Lead identification of 8-(methylamino)-2-oxo-1, 2-dihydroquinoline derivatives as DNA gyrase inhibitors: hit-to-lead generation involving thermodynamic evaluation","volume":"5","author":"Ushiyama","year":"2020","journal-title":"ACS Omega."},{"key":"ref102","doi-asserted-by":"publisher","first-page":"W356","DOI":"10.1093\/nar\/gkx374","article-title":"PharmMapper 2017 update: a web server for potential drug target identification with a comprehensive target pharmacophore database","volume":"45","author":"Wang","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"ref103","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1016\/j.jchromb.2016.02.030","article-title":"Simultaneous determination of fraxin and its metabolite, fraxetin, in rat plasma by liquid chromatography-tandem mass spectrometry and its application in a pharmacokinetic study","author":"Wang","year":"2016","journal-title":"J. Chromatogr. B"},{"key":"ref104","first-page":"315","article-title":"Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics","volume-title":"PCR protocols: A guide to methods and applications","author":"White","year":"1990"},{"key":"ref105","doi-asserted-by":"publisher","first-page":"624","DOI":"10.1038\/351624a0","article-title":"Crystal structure of an N-terminal fragment of the DNA gyrase B protein","volume":"351","author":"Wigley","year":"1991","journal-title":"Nature"},{"key":"ref106","doi-asserted-by":"publisher","first-page":"16014","DOI":"10.1038\/natrevmats.2016.14","article-title":"Analysis of nanoparticle delivery to tumours","volume":"1","author":"Wilhelm","year":"2016","journal-title":"Nat. Rev. Mater."},{"key":"ref107","doi-asserted-by":"publisher","first-page":"1076","DOI":"10.1002\/bmc.644","article-title":"Simultaneous determination of honokiol and magnolol in Magnolia officinalis by liquid chromatography with tandem mass spectrometric detection","volume":"20","author":"Wu","year":"2006","journal-title":"Biomed. Chromatogr."},{"key":"ref108","doi-asserted-by":"publisher","first-page":"2489","DOI":"10.3389\/fmicb.2019.02489","article-title":"Antibacterial effect and mode of action of flavonoids from licorice against methicillin-resistant Staphylococcus aureus","volume":"10","author":"Wu","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref109","doi-asserted-by":"publisher","first-page":"972294","DOI":"10.3389\/fmicb.2022.972294","article-title":"The novel distribution of intracellular and extracellular flavonoids produced by aspergillus sp. Gbtc 2, an endophytic fungus from Ginkgo biloba root","volume":"13","author":"Wu","year":"2022","journal-title":"Front. Microbiol."},{"key":"ref110","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1365\/s10337-010-1600-3","article-title":"A UPLC-MS-MS method for quantification of harpagoside and cinnamic acid in rat plasma and its application to a pharmacokinetic study after oral administration of Yanyan tablets","volume":"72","author":"Xiong","year":"2010","journal-title":"Chromatographia"},{"key":"ref111","doi-asserted-by":"publisher","first-page":"e1298","DOI":"10.1002\/wcms.1298","article-title":"Using PyMOL as a platform for computational drug design","volume":"7","author":"Yuan","year":"2017","journal-title":"Wiley Interdiscip. Rev. Comput. Mol. Sci."},{"key":"ref112","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1186\/s40104-019-0368-z","article-title":"Microbial synthesis of zinc oxide nanoparticles and their potential application as an antimicrobial agent and a feed supplement in animal industry: a review","volume":"10","author":"Yusof","year":"2019","journal-title":"J. Animal Sci. Biotechnol."},{"key":"ref113","doi-asserted-by":"publisher","first-page":"1911","DOI":"10.3390\/pr10101911","article-title":"Differential metabolomic fingerprinting of the crude extracts of three Asteraceae species with assessment of their in vitro antioxidant and enzyme-inhibitory activities supported by in silico investigations","volume":"10","author":"Zengin","year":"2022","journal-title":"Processes"},{"key":"ref114","doi-asserted-by":"publisher","first-page":"275","DOI":"10.2174\/2210315508666180501154759","article-title":"Chemistry and biology of secondary metabolites from aspergillus genus","volume":"8","author":"Zhang","year":"2018","journal-title":"Nat. Prod. J."},{"key":"ref115","doi-asserted-by":"publisher","first-page":"151","DOI":"10.3390\/molecules23010151","article-title":"Rapid screening and identification of daidzein metabolites in rats based on UHPLC-LTQ-Orbitrap mass spectrometry coupled with data-mining technologies","volume":"23","author":"Zhao","year":"2018","journal-title":"Molecules"}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2024.1366614\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T04:34:52Z","timestamp":1715574892000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2024.1366614\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,13]]},"references-count":115,"alternative-id":["10.3389\/fmicb.2024.1366614"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2024.1366614","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,13]]},"article-number":"1366614"}}