{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T02:19:57Z","timestamp":1780539597179,"version":"3.54.1"},"reference-count":33,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,4,22]],"date-time":"2024-04-22T00:00:00Z","timestamp":1713744000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Colitis caused by infections, especially <jats:italic>Salmonella<\/jats:italic>, has long been a common disease, underscoring the urgency to understand its intricate pathogenicity in colonic tissues for the development of effective anti-bacterial approaches. Of note, colonic epithelial cells, which form the first line of defense against bacteria, have received less attention, and the cross-talk between epithelial cells and bacteria requires further exploration. In this study, we revealed that the critical anti-bacterial effector, TFEB, was primarily located in colonic epithelial cells rather than macrophages. <jats:italic>Salmonella<\/jats:italic>-derived LPS significantly promoted the expression and nuclear translocation of TFEB in colonic epithelial cells by inactivating the mTOR signaling pathway <jats:italic>in vitro<\/jats:italic>, and this enhanced nuclear translocation of TFEB was also confirmed in a <jats:italic>Salmonella<\/jats:italic>-infected mouse model. Further investigation uncovered that the infection-activated TFEB contributed to the augmentation of anti-bacterial peptide expression without affecting the intact structure of the colonic epithelium or inflammatory cytokine expression. Our findings identify the preferential distribution of TFEB in colonic epithelial cells, where TFEB can be activated by infection to enhance anti-bacterial peptide expression, holding promising implications for the advancement of anti-bacterial therapeutics.<\/jats:p>","DOI":"10.3389\/fmicb.2024.1369471","type":"journal-article","created":{"date-parts":[[2024,4,22]],"date-time":"2024-04-22T05:02:50Z","timestamp":1713762170000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Colonic epithelial cell-specific TFEB activation: a key mechanism promoting anti-bacterial defense in response to Salmonella infection"],"prefix":"10.3389","volume":"15","author":[{"given":"Shanshan","family":"Rao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pu","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Yu","family":"Qian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongfeng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,4,22]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1584","DOI":"10.1080\/15548627.2019.1689770","article-title":"Restriction of intracellular Salmonella replication by restoring TFEB-mediated xenophagy","volume":"16","author":"Ammanathan","year":"2019","journal-title":"Autophagy"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"1165","DOI":"10.3748\/wjg.v20.i5.1165","article-title":"Intestinal barrier in inflammatory bowel disease","volume":"20","author":"Antoni","year":"2014","journal-title":"World J. 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