{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T23:08:14Z","timestamp":1778281694169,"version":"3.51.4"},"reference-count":61,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,7,31]],"date-time":"2024-07-31T00:00:00Z","timestamp":1722384000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Constipation is affected by a number of risk variables, including cardiovascular disease and growth factors. However, the impacts of gut flora on constipation incidence has not been shown. This work, Single-Variable Mendelian Randomization (SVMR) was utilized to estimate the causal relationship between the <jats:italic>Eubacterium genus<\/jats:italic> or <jats:italic>Rumphococcus<\/jats:italic>, and constipation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Data for constipation, <jats:italic>Eubacterium genus<\/jats:italic> and <jats:italic>Rumphococcus<\/jats:italic> were taken from the Integrated Epidemiology Unit (IEU) open GWAS database. Including 218,792 constipation samples, and there were 16,380,466 Single Nucleotide Polymorphisms (SNPs) for constipation. The ids of <jats:italic>Eubacterium genus<\/jats:italic> and <jats:italic>Rumphococcus<\/jats:italic> were sourced from MiBioGen database. The sample count for the <jats:italic>Eubacterium genus<\/jats:italic> was 17,380, with 656 SNPs. In addition, the sample size for <jats:italic>Rumphococcus<\/jats:italic> was 15,339, with 545 SNPs. The SVMR was performed to assess the risk of <jats:italic>Eubacterium genus<\/jats:italic> and <jats:italic>Rumphococcus<\/jats:italic> in constipation using weighted median, MR Egger, simple mode, inverse variance weighted (IVW), and weighted mode. Finally, we did a sensitivity analysis that included a heterogeneity, horizontal pleiotropy, and Leave-One-Out (LOO) test to examine the viability of the MR data.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The SVMR revealed that the <jats:italic>Eubacterium genus<\/jats:italic> and <jats:italic>Rumphococcus<\/jats:italic> were causally connected to constipation, with <jats:italic>Rumphococcus<\/jats:italic> (<jats:italic>P<\/jats:italic> = 0.042, OR = 1.074) as a hazardous factor and <jats:italic>Eubacterium genus<\/jats:italic> (<jats:italic>P<\/jats:italic> = 0.004, OR = 0.909) as a safety factor. Sensitivity tests then revealed the absence of variability between the constipation and the exposure factors (<jats:italic>Eubacterium genus<\/jats:italic> and <jats:italic>Rumphococcus<\/jats:italic>). Additionally, there were no other confounding factors and the examined SNPs could only influence constipation through the aforementioned exposure factors, respectively. As a result, the MR results were fairly robust.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our investigation verified the causal links between the <jats:italic>Eubacterium genus<\/jats:italic> or <jats:italic>Rumphococcus<\/jats:italic>, and constipation, with greater <jats:italic>Rumphococcus<\/jats:italic> expression increasing the likelihood of constipation and the opposite being true for the <jats:italic>Eubacterium genus<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2024.1376232","type":"journal-article","created":{"date-parts":[[2024,7,31]],"date-time":"2024-07-31T17:44:57Z","timestamp":1722447897000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Assessing the causal effects of Eubacterium and Rumphococcus on constipation: a Mendelian randomized study"],"prefix":"10.3389","volume":"15","author":[{"given":"Xiao","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenchun","family":"Du","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianhao","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2024,7,31]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"510","DOI":"10.1111\/nmo.12295","article-title":"Probiotic gut effectprevents the chronic psychological stress-induced brain activity abnormality in mice","volume":"26","author":"Ait-Belgnaoui","year":"2014","journal-title":"Neurogastroenterol. 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