{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T11:27:06Z","timestamp":1782991626771,"version":"3.54.5"},"reference-count":37,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,3,26]],"date-time":"2024-03-26T00:00:00Z","timestamp":1711411200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p><jats:italic>Sclerotinia sclerotiorum<\/jats:italic> is a known pathogen that harms crops and vegetables. Unfortunately, there is a lack of effective biological control measures for this pathogen. <jats:italic>Bacillus velezensis<\/jats:italic> 20507 has a strong antagonistic effect on <jats:italic>S. Sclerotiorum<\/jats:italic>; however, the biological basis of its antifungal effect is not fully understood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, the broad-spectrum antagonistic microorganisms of <jats:italic>B. velezensis<\/jats:italic> 20507 were investigated, and the active antifungal ingredients in this strain were isolated, purified, identified and thermal stability experiments were carried out to explore its antifungal mechanism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The <jats:italic>B. velezensis<\/jats:italic> 20507 genome comprised one circular chromosome with a length of 4,043,341 bp, including 3,879 genes, 185 tandem repeats, 87 tRNAs, and 27 rRNAs. Comparative genomic analysis revealed that our sequenced strain had the closest genetic relationship with <jats:italic>Bacillus velezensis<\/jats:italic> (GenBank ID: NC 009725.2); however, there were significant differences in the positions of genes within the two genomes. It is predicted that <jats:italic>B. velezensis<\/jats:italic> 20507 encode 12 secondary metabolites, including difficidin, macrolactin H, fengycin, surfactin, bacillibactin, bacillothiazole A-N, butirosin a\/b, and bacillaene. Results showed that <jats:italic>B. velezensis<\/jats:italic> 20507 produced various antagonistic effects on six plant pathogen strains: <jats:italic>Exserohilum turcicum, Pyricularia oryzae, Fusarium graminearum, Sclerotinia sclerotiorum, Fusarium oxysporum<\/jats:italic>, and <jats:italic>Fusarium verticillioides<\/jats:italic>. Acid precipitation followed by 80% methanol leaching is an effective method for isolating the antifungal component ME80 in <jats:italic>B. velezensis<\/jats:italic> 20507, which can damage the membranes of <jats:italic>S. sclerotiorum<\/jats:italic> hyphae and has good heat resistance. Using high-performance liquid chromatography, and Mass Spectrometry analysis, it is believed that fengycin C72H110N12O20 is the main active antifungal substance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>This study provides new resources for the biological control of <jats:italic>S. Sclerotiorum<\/jats:italic> in soybeans and a theoretical basis for further clarification of the mechanism of action of <jats:italic>B. velezensis<\/jats:italic> 20507.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2024.1385067","type":"journal-article","created":{"date-parts":[[2024,3,26]],"date-time":"2024-03-26T04:51:35Z","timestamp":1711428695000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":15,"title":["Genomic and biological control of Sclerotinia sclerotiorum using an extracellular extract from Bacillus velezensis 20507"],"prefix":"10.3389","volume":"15","author":[{"given":"Yunqing","family":"Cheng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanxiao","family":"Lou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongli","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyi","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zicheng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianfeng","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,3,26]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9091893","article-title":"Stimulation of Nicotiana tabacum L. 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