{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T14:06:36Z","timestamp":1775829996808,"version":"3.50.1"},"reference-count":85,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T00:00:00Z","timestamp":1719360000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Bacteria employ small regulatory RNAs (sRNA) and\/or RNA binding proteins (RBPs) to respond to environmental cues. In <jats:italic>Enterobacteriaceae<\/jats:italic>, the FinO-domain containing RBP ProQ associates with numerous sRNAs and mRNAs, impacts sRNA-mediated riboregulation or mRNA stability by binding to 5\u2032- or 3\u2032-untranslated regions as well as to internal stem loop structures. Global RNA-protein interaction studies and sequence comparisons identified a ProQ-like homolog (PA2582\/ProQ<jats:italic><jats:sub>Pae<\/jats:sub><\/jats:italic>) in <jats:italic>Pseudomonas aeruginosa<\/jats:italic> (<jats:italic>Pae<\/jats:italic>). To address the function of ProQ<jats:italic><jats:sub>Pae<\/jats:sub><\/jats:italic>, at first a comparative transcriptome analysis of the <jats:italic>Pae<\/jats:italic> strains PAO1 and PAO1\u0394<jats:italic>proQ<\/jats:italic> was performed. This study revealed more than 100 differentially abundant transcripts, affecting a variety of cellular functions. Among these transcripts were <jats:italic>pprA<\/jats:italic> and <jats:italic>pprB<\/jats:italic>, encoding the PprA\/PprB two component system, <jats:italic>psrA<\/jats:italic>, encoding a transcriptional activator of <jats:italic>pprB<\/jats:italic>, and <jats:italic>oprI<\/jats:italic>, encoding the outer membrane protein OprI. RNA co-purification experiments with Strep-tagged <jats:italic>Pae<\/jats:italic> ProQ protein corroborated an association of ProQ<jats:italic><jats:sub>Pae<\/jats:sub><\/jats:italic> with these transcripts. In accordance with the up-regulation of the <jats:italic>psrA<\/jats:italic>, <jats:italic>pprA<\/jats:italic>, and <jats:italic>pprB<\/jats:italic> genes in strain PAO1\u0394<jats:italic>proQ<\/jats:italic> a phenotypic analysis revealed an increased susceptibility toward the aminoglycosides tobramycin and gentamicin in biofilms. Conversely, the observed down-regulation of the <jats:italic>oprI<\/jats:italic> gene in PAO1\u0394<jats:italic>proQ<\/jats:italic> could be reconciled with a decreased susceptibility toward the synthetic cationic antimicrobial peptide GW-Q6. Taken together, these studies revealed that ProQ<jats:italic><jats:sub>Pae<\/jats:sub><\/jats:italic> is an RBP that impacts antimicrobial resistance in <jats:italic>Pae<\/jats:italic>.<\/jats:p>","DOI":"10.3389\/fmicb.2024.1422742","type":"journal-article","created":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T04:37:50Z","timestamp":1719376670000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["The FinO\/ProQ-like protein PA2582 impacts antimicrobial resistance in Pseudomonas aeruginosa"],"prefix":"10.3389","volume":"15","author":[{"given":"Anastasia","family":"Cianciulli Sesso","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Armin","family":"Resch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabella","family":"Moll","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Udo","family":"Bl\u00e4si","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabeth","family":"Sonnleitner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2024,6,26]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"350","DOI":"10.1016\/j.drudis.2018.07.003","article-title":"Updates on the pathogenicity status of Pseudomonas aeruginosa","volume":"24","author":"Azam","year":"2019","journal-title":"Drug Discov. 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