{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:42:20Z","timestamp":1790836940499,"version":"4.1.0"},"reference-count":43,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T00:00:00Z","timestamp":1719964800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p><jats:italic>Candida auris<\/jats:italic> is a recently discovered yeast with a multi-drug resistant profile associated with high mortality rates. The rapid identification of <jats:italic>Candida auris<\/jats:italic> in hospital settings is crucial to allow appropriate therapeutic and rapid implementation of infection management measures. The aim of this study was to develop a lateral flow immunoassay (LFIA) for the rapid identification of <jats:italic>Candida auris<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Highly specific monoclonal antibodies were obtained by immunizing mice with membrane proteins from <jats:italic>Candida auris<\/jats:italic> which were then used to develop a LFIA whose performance was assessed by testing 12 strains of <jats:italic>Candida auris<\/jats:italic> and 37 strains of other Candida species. Isolates were grown on either Sabouraud dextrose, CHROMagar<jats:sup>TM<\/jats:sup> Candida Plus or HardyCHROM<jats:sup>TM<\/jats:sup><jats:italic>Candida<\/jats:italic> + <jats:italic>auris<\/jats:italic> agar plates. The strains were also cultured on salt sabouraud-dextrose with chloramphenicol or a commercially available Salt-Sabouraud Dulcitol Broth with chloramphenicol and gentamicin, and processed using a simple centrifugation protocol to recover a pellet. Finally, the colonies or yeast extract were transferred to the LFIA to determine the specificity and sensitivity of the assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The LFIA reached 100% specificity and sensitivity from solid agar plates. For both enrichment broths, some Candida non-auris species were able to grow, but the LFIA remained 100% specific. The use of a dextrose-based sabouraud broth resulted in earlier identification with the LFIA, with most of the Candida auris strains detected at 24 h.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The developed LFIA prototype represents a powerful tool to fight the emerging threat of Candida auris. Clinical validation represents the next step.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2024.1439273","type":"journal-article","created":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T05:27:05Z","timestamp":1719984425000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["A lateral flow immunoassay for the rapid identification of Candida auris from isolates or directly from surveillance enrichment broths"],"prefix":"10.3389","volume":"15","author":[{"given":"Arnaud","family":"Chalin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoine","family":"Arvor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Sophie","family":"Hervault","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Plaisance","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e9a","family":"Niol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Simon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herv\u00e9","family":"Volland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,7,3]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1007\/978-1-0716-2417-3_2","article-title":"Candida auris identification and profiling by MALDI-ToF mass spectrometry","volume":"2517","author":"Abdolrasouli","year":"2022","journal-title":"Methods Mol. 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