{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T17:40:21Z","timestamp":1793468421377,"version":"4.5.0"},"reference-count":50,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,9,16]],"date-time":"2024-09-16T00:00:00Z","timestamp":1726444800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>This study aimed to investigate the effects of the cell-free supernatant of <jats:italic>Lactiplantibacillus plantarum<\/jats:italic> ATCC<jats:sup>\u00ae<\/jats:sup> 10241<jats:sup>TM<\/jats:sup> on the biofilm-forming capacity of <jats:italic>Pseudomonas aeruginosa<\/jats:italic> strains isolated from cystic fibrosis (CF) patients. In addition, the study evaluated the <jats:italic>in vivo<\/jats:italic> potential of the cell-free supernatant to modulate inflammation and reduce lung damage in mice infected with <jats:italic>P. aeruginosa<\/jats:italic> strains or co-challenged with <jats:italic>P. aeruginosa<\/jats:italic> and the <jats:italic>Streptococcus milleri<\/jats:italic> group (SMG). The results showed that CF-derived <jats:italic>P. aeruginosa<\/jats:italic> strains can infect the respiratory tract of adult mice, inducing local inflammation and lung damage. The severity of these infections was exacerbated when <jats:italic>P. aeruginosa<\/jats:italic> was co-administered with SMG. Notably, nebulization with the cell-free supernatant of <jats:italic>L. plantarum<\/jats:italic> produced beneficial effects, reducing respiratory infection severity and inflammatory responses induced by <jats:italic>P. aeruginosa<\/jats:italic>, both alone or in combination with SMG. Reduced bacterial loads and lung damage were observed in supernatant-treated mice compared to controls. Although further mechanistic studies are necessary, the results show that the cell-free supernatant of <jats:italic>L. plantarum<\/jats:italic> ATCC<jats:sup>\u00ae<\/jats:sup> 10241<jats:sup>TM<\/jats:sup> is an interesting adjuvant alternative to treat <jats:italic>P. aeruginosa<\/jats:italic> respiratory infections and superinfections in CF patients.<\/jats:p>","DOI":"10.3389\/fmicb.2024.1440090","type":"journal-article","created":{"date-parts":[[2024,9,16]],"date-time":"2024-09-16T04:44:22Z","timestamp":1726461862000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["Effect of Lactiplantibacillus plantarum cell-free culture on bacterial pathogens isolated from cystic fibrosis patients: in vitro and in vivo studies"],"prefix":"10.3389","volume":"15","author":[{"given":"Carla Luciana","family":"Ab\u00e1n","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Orosco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio Nicol\u00e1s","family":"Arga\u00f1araz Aybar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo","family":"Albarrac\u00edn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anal\u00eda","family":"Venecia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liliana","family":"Perret","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Ortiz Mayor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keita","family":"Nishiyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Carlos","family":"Vald\u00e9z","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haruki","family":"Kitazawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio","family":"Villena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia","family":"Gobbato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,9,16]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"634","DOI":"10.3390\/microorganisms10030634","article-title":"Topical administration of Lactiplantibacillus plantarum accelerates the healing of chronic diabetic foot ulcers through modifications of infection, angiogenesis, macrophage phenotype and neutrophil response","volume":"10","author":"Aybar","year":"2022","journal-title":"Microorganisms"},{"key":"B2","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1007\/s00203-022-02770-8","article-title":"Postbiotics of Lactobacillus casei target virulence and biofilm formation of Pseudomonas aeruginosa by modulating quorum sensing","volume":"204","author":"Azami","year":"2022","journal-title":"Archiv. Microbiol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41467-019-08504-7","article-title":"Evolutionary highways to persistent bacterial infection","volume":"10","author":"Bartell","year":"2019","journal-title":"Nat. Commun."},{"key":"B4","doi-asserted-by":"publisher","first-page":"71158","DOI":"10.3390\/antibiotics12071158","article-title":"Antibacterial and antibiofilm effects of lactobacilli strains against clinical isolates of Pseudomonas aeruginosa under conditions relevant to cystic fibrosis","volume":"12","author":"Batoni","year":"2023","journal-title":"Antibiotics"},{"key":"B5","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1128\/IAI.00237-20","article-title":"Scnn1b-Transgenic BALB\/c mice as a model of Pseudomonas aeruginosa infections of the cystic fibrosis lung","volume":"88","author":"Brao","year":"2020","journal-title":"Infect. Immun."},{"key":"B6","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1093\/jac\/dki217","article-title":"Comparative assessment of antibiotic susceptibility of coagulase-negative staphylococci in biofilm versus planktonic culture as assessed by bacterial enumeration or Rapid XTT Colorimetry","volume":"56","author":"Cerca","year":"2005","journal-title":"J. Antimicrob. Chemother."},{"key":"B7","doi-asserted-by":"publisher","first-page":"266735","DOI":"10.3389\/fimmu.2017.00948","article-title":"Peptidoglycan from immunobiotic Lactobacillus rhamnosus improves resistance of infant mice to respiratory syncytial viral infection and secondary pneumococcal pneumonia","volume":"8","author":"Clua","year":"2017","journal-title":"Front. Immunol."},{"key":"B8","doi-asserted-by":"publisher","first-page":"1653","DOI":"10.3390\/cells9071653","article-title":"The role of alveolar macrophages in the improved protection against respiratory syncytial virus and pneumococcal superinfection induced by the peptidoglycan of Lactobacillus rhamnosus CRL1505","volume":"9","author":"Clua","year":"2020","journal-title":"Cells"},{"key":"B9","doi-asserted-by":"publisher","first-page":"63","DOI":"10.36106\/IJSR","article-title":"Bacteria-bacteria and host-bacteria interaction of bacteria isolated from a patient with cystic fibrosis","volume":"7","author":"Delgado","year":"2018","journal-title":"Int. J. Sci. Res."},{"key":"B10","doi-asserted-by":"publisher","first-page":"597","DOI":"10.1080\/08927014.2020.1783253","article-title":"Human probiotic bacteria attenuate Pseudomonas aeruginosa biofilm and virulence by quorum-sensing inhibition","volume":"36","author":"D\u00edaz","year":"2020","journal-title":"Biofouling"},{"key":"B11","doi-asserted-by":"publisher","first-page":"1477","DOI":"10.1046\/j.1365-2958.2003.03803.x","article-title":"Modulation of Pseudomonas aeruginosa gene expression by host microflora through interspecies communication","volume":"50","author":"Duan","year":"2003","journal-title":"Mol. Microbiol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"15773","DOI":"10.3390\/ijms242115773","article-title":"Immunobiotic Ligilactobacillus salivarius FFIG58 confers long-term protection against Streptococcus pneumoniae","volume":"24","author":"Elean","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"B13","doi-asserted-by":"publisher","first-page":"2416","DOI":"10.3389\/fimmu.2018.02416","article-title":"Pseudomonas aeruginosa in chronic lung infections: how to adapt within the host?","volume":"9","author":"Faure","year":"2018","journal-title":"Front. Immunol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"3000","DOI":"10.1128\/jb.173.9.3000-3009.1991","article-title":"Cloning and characterization of the Pseudomonas aeruginosa LasR Gene, a transcriptional activator of elastase expression","volume":"173","author":"Gambello","year":"1991","journal-title":"J. Bacteriol."},{"key":"B15","doi-asserted-by":"publisher","first-page":"918","DOI":"10.1164\/rccm.200304-505SO","article-title":"Pathophysiology and management of pulmonary infections in cystic fibrosis","volume":"168","author":"Gibson","year":"2003","journal-title":"Am. J. Respirat. Crit. Care Med."},{"key":"B16","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1128\/CMR.00031-19","article-title":"Epidemiology and treatment of multidrug-resistant and extensively drug-resistant Pseudomonas aeruginosa infections","volume":"32","author":"Horcajada","year":"2019","journal-title":"Clin. Microbiol. Rev."},{"key":"B17","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41467-019-11414-3","article-title":"Genetically diverse Pseudomonas aeruginosa populations display similar transcriptomic profiles in a cystic fibrosis explanted lung","volume":"10","author":"Kordes","year":"2019","journal-title":"Nat. Commun."},{"key":"B18","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1007\/978-3-031-08491-1_5","article-title":"Antibiotic resistance in pseudomonas","volume":"1386","author":"Laborda","year":"2022","journal-title":"Adv. Exp. Med. Biol."},{"key":"B19","doi-asserted-by":"publisher","first-page":"2113","DOI":"10.1097\/CCM.0b013e31821e899f","article-title":"Relative contribution of three main virulence factors in Pseudomonas aeruginosa pneumonia","volume":"39","author":"Le Berre","year":"2011","journal-title":"Crit. Care Med."},{"key":"B20","volume-title":"A Short Course in Bacterial Genetics: A Laboratory Manual and Handbook for Escherichia coli and Related Bacteria","author":"Miller","year":"1992"},{"key":"B21","doi-asserted-by":"publisher","first-page":"394","DOI":"10.1097\/MPG.0000000000002185","article-title":"Systematic review of probiotics for cystic fibrosis patients: moving forward","volume":"68","author":"Neri","year":"2019","journal-title":"J. Pediatr. Gastroenterol. Nutr."},{"key":"B22","doi-asserted-by":"publisher","first-page":"449","DOI":"10.1046\/j.1365-2958.1998.00797.x","article-title":"Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis","volume":"28","author":"O'Toole","year":"1998","journal-title":"Mol. Microbiol."},{"key":"B23","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1128\/mBio.00500-17","article-title":"Metabolic compensation of fitness costs is a general outcome for antibiotic-resistant Pseudomonas aeruginosa mutants overexpressing efflux pumps","volume":"8","author":"Pacheco","year":"2017","journal-title":"MBio"},{"key":"B24","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1111\/j.1742-481X.2008.00577.x","article-title":"Bacteriotherapy with Lactobacillus plantarum in burns","volume":"6","author":"Peral","year":"2009","journal-title":"Int. Wound J."},{"key":"B25","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1111\/j.1469-0691.2009.02793.x","article-title":"Interleukin-8 production by polymorphonuclear leukocytes from patients with chronic infected leg ulcers treated with Lactobacillus plantarum","volume":"16","author":"Peral","year":"2010","journal-title":"Clin. Microbiol. Infect."},{"key":"B26","doi-asserted-by":"publisher","first-page":"105301","DOI":"10.1016\/j.micinf.2024.105301","article-title":"cell-free supernatants from lactobacillus strains exert antibacterial, antibiofilm, and antivirulence activity against Pseudomonas aeruginosa from cystic fibrosis patients","volume":"2024","author":"Pompilio","year":"2024","journal-title":"Microbes Infect"},{"key":"B27","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1128\/JB.00705-15","article-title":"Tobramycin-treated Pseudomonas aeruginosa PA14 enhances Streptococcus constellatus 7155 biofilm formation in a cystic fibrosis model system","volume":"198","author":"Price","year":"2015","journal-title":"J. Bacteriol."},{"key":"B28","doi-asserted-by":"publisher","first-page":"96914","DOI":"10.1172\/jci.insight.96914","article-title":"Thrombospondin-1 protects against pathogen-induced lung injury by limiting extracellular matrix proteolysis","volume":"3","author":"Qu","year":"2018","journal-title":"JCI Insight"},{"key":"B29","doi-asserted-by":"publisher","first-page":"350","DOI":"10.3109\/13880209.2014.920037","article-title":"Compounds from Lactobacillus plantarum culture supernatants with potential pro-healing and anti-pathogenic properties in skin chronic wounds","volume":"53","author":"Ramos","year":"2015","journal-title":"Pharmaceut. Biol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/j.1574-6968.2005.00001.x","article-title":"Quorum sensing: the many languages of bacteria","volume":"254","author":"Reading","year":"2006","journal-title":"FEMS Microbiol. Lett."},{"key":"B31","doi-asserted-by":"publisher","first-page":"2117","DOI":"10.1007\/s40265-021-01635-6","article-title":"The epidemiology and pathogenesis and treatment of Pseudomonas aeruginosa infections: an update","volume":"81","author":"Reynolds","year":"2021","journal-title":"Drugs"},{"key":"B32","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41467-018-05944-5","article-title":"High-resolution in situ transcriptomics of Pseudomonas aeruginosa unveils genotype independent patho-phenotypes in cystic fibrosis lungs","volume":"9","author":"Rossi","year":"2018","journal-title":"Nat. Commun."},{"key":"B33","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1038\/s41579-020-00477-5","article-title":"Pseudomonas aeruginosa adaptation and evolution in patients with cystic fibrosis","volume":"19","author":"Rossi","year":"2020","journal-title":"Nat. Rev. Microbiol."},{"key":"B34","doi-asserted-by":"publisher","first-page":"e2529","DOI":"10.1093\/cid\/ciaa765","article-title":"Pseudomonas aeruginosa and children with cystic fibrosis","volume":"73","author":"Sala","year":"2021","journal-title":"Clin. Infect. Dis."},{"key":"B35","doi-asserted-by":"publisher","first-page":"2066","DOI":"10.1128\/JB.185.7.2066-2079.2003","article-title":"Identification, timing, and signal specificity of Pseudomonas aeruginosa quorum-controlled genes: a transcriptome analysis","volume":"185","author":"Schuster","year":"2003","journal-title":"J. Bacteriol."},{"key":"B36","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1128\/JB.00115-19","article-title":"The yin and yang of streptococcus lung infections in cystic fibrosis: a model for studying polymicrobial interactions","volume":"201","author":"Scott","year":"2019","journal-title":"J. Bacteriol."},{"key":"B37","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1007\/s12602-017-9267-9","article-title":"The inhibition effect of lactobacilli against growth and biofilm formation of Pseudomonas aeruginosa","volume":"10","author":"Shokri","year":"2018","journal-title":"Probiot. Antimicrob. Proteins"},{"key":"B38","doi-asserted-by":"publisher","first-page":"15070","DOI":"10.1073\/pnas.0804326105","article-title":"A polymicrobial perspective of pulmonary infections exposes an enigmatic pathogen in cystic fibrosis patients","volume":"105","author":"Sibley","year":"2008","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"B39","doi-asserted-by":"publisher","first-page":"20747","DOI":"10.1073\/pnas.1221552110","article-title":"enhanced in vivo fitness of carbapenem-resistant OprD mutants of Pseudomonas aeruginosa revealed through high-throughput sequencing","volume":"110","author":"Skurnik","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"B40","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1590\/s1678-9946202163071","article-title":"Carbapenem-resistant Pseudomonas aeruginosa strains: a worrying health problem in intensive care units","volume":"63","author":"Souza","year":"2021","journal-title":"Revista Do Instituto de Medicina Tropical de Sao Paulo"},{"key":"B41","doi-asserted-by":"publisher","first-page":"e73","DOI":"10.1016\/j.ijid.2009.11.019","article-title":"Influence of carbapenem resistance on mortality and the dynamics of mortality in Pseudomonas aeruginosa bloodstream infection","volume":"14","author":"Su\u00e1rez","year":"2010","journal-title":"Int. J. Infect. Dis."},{"key":"B42","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1128\/iai.64.1.37-43.1996","article-title":"Contribution of specific Pseudomonas aeruginosa virulence factors to pathogenesis of pneumonia in a neonatal mouse model of infection","volume":"64","author":"Tang","year":"1996","journal-title":"Infect. Immun."},{"key":"B43","doi-asserted-by":"publisher","first-page":"209","DOI":"10.3920\/BM2022.0118","article-title":"Post-immunobiotics increase resistance to primary respiratory syncytial virus infection and secondary pneumococcal pneumonia","volume":"14","author":"Tonetti","year":"2023","journal-title":"Beneficial Microbes"},{"key":"B44","doi-asserted-by":"publisher","first-page":"1","DOI":"10.12688\/f1000research.19509.1","article-title":"Emerging therapies against infections with Pseudomonas aeruginosa","volume":"8","author":"T\u00fcmmler","year":"2019","journal-title":"F1000Research"},{"key":"B45","doi-asserted-by":"publisher","first-page":"472","DOI":"10.1111\/j.1469-0691.2005.01142.x","article-title":"Interference of Lactobacillus plantarum with Pseudomonas aeruginosa in vitro and in infected burns: the potential use of probiotics in wound treatment","volume":"11","author":"Vald\u00e9z","year":"2005","journal-title":"Clin. Microbiol. Infect."},{"key":"B46","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1111\/j.1600-0463.2009.02466.x","article-title":"Inactivation of the RhlA gene in Pseudomonas aeruginosa prevents rhamnolipid production, disabling the protection against polymorphonuclear leukocytes","volume":"117","author":"Van Gennip","year":"2009","journal-title":"APMIS"},{"key":"B47","doi-asserted-by":"publisher","first-page":"699","DOI":"10.3389\/fphys.2020.00699","article-title":"The modulation of mucosal antiviral immunity by immunobiotics: could they offer any benefit in the SARS-CoV-2 pandemic?","volume":"11","author":"Villena","year":"2020","journal-title":"Front. Physiol."},{"key":"B48","doi-asserted-by":"publisher","first-page":"e0173741","DOI":"10.1371\/journal.pone.0173741","article-title":"Modulation of behaviour and virulence of a high alginate expressing Pseudomonas aeruginosa strain from cystic fibrosis by oral commensal bacterium Streptococcus anginosus","volume":"12","author":"Waite","year":"2017","journal-title":"PLoS ONE"},{"key":"B49","doi-asserted-by":"publisher","first-page":"13904","DOI":"10.1073\/pnas.96.24.13904","article-title":"Identification of genes controlled by quorum sensing in Pseudomonas aeruginosa","volume":"96","author":"Whiteley","year":"1999","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"B50","doi-asserted-by":"publisher","first-page":"1624","DOI":"10.1016\/j.chest.2021.04.015","article-title":"Elastase activity from Pseudomonas aeruginosa respiratory isolates and ICU mortality","volume":"160","author":"Zupetic","year":"2021","journal-title":"Chest"}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2024.1440090\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,16]],"date-time":"2024-09-16T04:44:40Z","timestamp":1726461880000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2024.1440090\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,16]]},"references-count":50,"alternative-id":["10.3389\/fmicb.2024.1440090"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2024.1440090","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,16]]},"article-number":"1440090"}}