{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T16:08:31Z","timestamp":1793376511485,"version":"4.5.0"},"reference-count":49,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T00:00:00Z","timestamp":1733961600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    The emergence of the multidrug-resistant (MDR)\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    ST131 clone has significantly impacted public health. With traditional antibiotics becoming less effective against MDR bacteria, there is an urgent need for alternative treatment options. This study aimed to isolate and characterize four lytic phages (EC.W2-1, EC.W2-6, EC.W13-3, and EC.W14-3) from hospital sewage water and determine their effectiveness against the ST131 clone. These phages demonstrated a broad host range, effectively lysing 94.4% of highly pathogenic\n                    <jats:italic>E. coli<\/jats:italic>\n                    ST131 isolates. Morphological observations and phylogenetic analysis indicate that EC.W2-1, and EC.W13-3 belong to the\n                    <jats:italic>Tequatrovirus<\/jats:italic>\n                    genus in the\n                    <jats:italic>Straboviridae<\/jats:italic>\n                    family, while EC.W2-6 and EC.W14-3 are part of the\n                    <jats:italic>Kuravirus<\/jats:italic>\n                    genus in the\n                    <jats:italic>Podoviridae<\/jats:italic>\n                    family. Phages remained stable at pH 2\u201310 for 4\u202fh and below 80\u00b0C for 1\u202fh. These four phages showed\n                    <jats:italic>in vitro<\/jats:italic>\n                    bacterial lytic activity at various MOIs (0.1\u20130.001). The one-step growth curve of phages exhibited a short latent period of approximately 10\u201320\u202fmin and a moderate burst size of 50\u201380 (pfu\/cell). Phages\u2019 genome size ranged from 46,325\u2013113,909\u202fbp, with G\u202f+\u202fC content of 35.1 \u201338.3%. No virulence or drug resistance genes were found, which enhanced their safety profile.\n                    <jats:italic>In vivo<\/jats:italic>\n                    , EC.W2-6 and EC.W13.3, along with their cocktail, fully protected against the ESBL-producing\n                    <jats:italic>E. coli<\/jats:italic>\n                    ST131 infection model\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Combining these phages and a 3-day repeated single phage, EC.W13-3 significantly enhanced the survival rate of\n                    <jats:italic>E. coli<\/jats:italic>\n                    ST131 infected mice at low MOI (0.01\u20130.001). The\n                    <jats:italic>in vivo<\/jats:italic>\n                    effectiveness of the isolated phages and the EC.W2-6 and EC.W14-3 cocktail in highly reducing bacterial load CFU\/g in multiple organs strongly supports their potential efficacy. Based on\n                    <jats:italic>in vivo<\/jats:italic>\n                    ,\n                    <jats:italic>in vitro<\/jats:italic>\n                    , and genomic analyses, phages have been proposed as novel and suitable candidates for killing the pandemic ST131 clone.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2024.1455710","type":"journal-article","created":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T13:16:21Z","timestamp":1734009381000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":14,"title":["Bacteriophage as a novel therapeutic approach for killing multidrug-resistant Escherichia coli ST131 clone"],"prefix":"10.3389","volume":"15","author":[{"given":"Md","family":"Shamsuzzaman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shukho","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jungmin","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,12,12]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"455","DOI":"10.3343\/ALM.2021.41.5.455","article-title":"Risk factors for extended-Spectrum-\u03b2-lactamase-producing Escherichia coli in community-onset bloodstream infection: impact on long-term care hospitals in Korea","volume":"41","author":"Baek","year":"2021","journal-title":"Ann. 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