{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T03:08:20Z","timestamp":1790392100259,"version":"4.1.0"},"reference-count":60,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T00:00:00Z","timestamp":1727136000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Environmental soil contamination is a serious problem for humans worldwide, as it causes many diseases.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The present study focuses on utilizing biosurfactants produced by\n                      <jats:italic>Pseudomonas stutzeri<\/jats:italic>\n                      (\n                      <jats:italic>P. stutzeri<\/jats:italic>\n                      ) NA3 and\n                      <jats:italic>Bacillus cereus<\/jats:italic>\n                      (\n                      <jats:italic>B. cereus<\/jats:italic>\n                      ) EN6, as an electrolyte for removing chromium (Cr) from contaminated soil using the electrokinetic (EK) process.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      As a result, biosurfactants produced by\n                      <jats:italic>P. stutzeri<\/jats:italic>\n                      NA3 and\n                      <jats:italic>B. cereus<\/jats:italic>\n                      EN6, being lipopeptides, increase heavy metal mobility in the EK process. The Cr removal efficiency of a novel electrolyte (biosurfactants) in the EK process was compared with that of NA3 and EN6 biosurfactants. The EK results revealed a maximum Cr removal of 75 and 70% by NA3 and EN6, respectively, at the end of 7\u2009days.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>The biosurfactant aids in the breaking down of the heavy metals that are present deeper into the soil matrix. From the metagenomics analysis, it was identified that biosurfactant changes the microbial community with an enhanced ability to remove heavy metals. The phytotoxicity assay confirms that NA3 biosurfactant solution showed 95% seed germination and can lower hazardous pollutants in the soil.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The application of biosurfactants as a potent electrolyte for the remediation of hazardous pollutants is an integrated process. Overall, the results of this study suggest that biosurfactants can serve as an economic and efficient electrolyte in the EK process to remove Cr from polluted soil.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2024.1458369","type":"journal-article","created":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T00:44:32Z","timestamp":1727138672000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":14,"title":["Biosurfactant-assisted bio-electrokinetic enhanced remediation of heavy metal-contaminated soil"],"prefix":"10.3389","volume":"15","author":[{"given":"Jayaraman","family":"Narenkumar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhaskar","family":"Das","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subramani","family":"Abilaji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuppusamy","family":"Sathishkumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamad S.","family":"AlSalhi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandhanasamy","family":"Devanesan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aruliah","family":"Rajasekar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tabarak","family":"Malik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,9,24]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"140516","DOI":"10.1016\/j.chemosphere.2023.140516","article-title":"Electrochemical oxidation of azo dyes degradation by RuO2\u2013IrO2\u2013TiO2 electrode with biodegradation Aeromonas hydrophila AR1 and its degradation pathway: an integrated approach","volume":"345","author":"Abilaji","year":"","journal-title":"Chemosphere"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"138816","DOI":"10.1016\/j.chemosphere.2023.138816","article-title":"Sequential photo electro oxidation and biodegradation of textile effluent: elucidation of degradation mechanism and bacterial diversity","volume":"331","author":"Abilaji","year":"","journal-title":"Chemosphere"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"2638","DOI":"10.1021\/es00049a002","article-title":"Principles of electrokinetic remediation","volume":"27","author":"Acar","year":"1993","journal-title":"Environ. Sci. Technol."},{"key":"ref002","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1061\/(ASCE)1090-0241(2008)134:1(91)","article-title":"Electrokinetic remediation modeling incorporating geochemical effects","volume":"134","author":"Al-Hamdan","year":"2008","journal-title":"Journal of geotechnical and geoenvironmental engineering"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"2595","DOI":"10.1007\/s13762-017-1488-7","article-title":"Chromium (VI) adsorption by Codium tomentosum: evidence for adsorption by porous media from sigmoidaldose\u2013response curve","volume":"15","author":"Anandaraj","year":"2017","journal-title":"Int. J. Environ. Sci. Technol."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/j.colsurfb.2012.01.043","article-title":"Production and characterization of biosurfactant produced by a novel Pseudomonas sp. 2B","volume":"95","author":"Aparna","year":"2012","journal-title":"Colloids Surf. B"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"19660","DOI":"10.1038\/s41598-020-75170-x","article-title":"Genomic analysis of Bacillus cereus NWUAB01 and its heavy metal removal from polluted soils","volume":"10","author":"Ayangbenro","year":"2020","journal-title":"Sci. Rep."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"9237","DOI":"10.1038\/s41598-020-65534-8","article-title":"Redox mechanisms of conversion of Cr (VI) to Cr (III) by graphene oxide-polymer composite","volume":"10","author":"Bandara","year":"2020","journal-title":"Sci. Rep."},{"key":"ref8","first-page":"3484","article-title":"Structural analysis and characterization of biosurfactants produced by some crude oil utilizing bacterial strains","volume":"4","author":"Bayoumi","year":"2010","journal-title":"Aust. J. Basic Appl. Sci."},{"key":"ref008","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1007\/s11270-024-07120-6","article-title":"Chromium (VI) Reduction, and Antibacterial Efficacy","volume":"235","author":"Blue","year":"","journal-title":"Water, Air, Soil Pollut."},{"key":"ref005","doi-asserted-by":"crossref","first-page":"1799","DOI":"10.3390\/app11041799","article-title":"Enhanced electrokinetic remediation for the removal of heavy metals from contaminated soils","volume":"11","author":"Cameselle","year":"2021","journal-title":"Applied Sciences"},{"key":"ref9","first-page":"398","article-title":"Isolation and characterization of fatty acid esters and phosphatidylethanolamine surfactants from a consortium of marine bacteria","volume":"41","author":"Deshmukh","year":"2012","journal-title":"Indian J. Geomarine sci."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"139192","DOI":"10.1016\/j.chemosphere.2023.139192","article-title":"Microalgae: a green eco-friendly agents for bioremediation of tannery wastewater with simultaneous production of value-added products","volume":"336","author":"Devi","year":"2023","journal-title":"Chemosphere"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"1800","DOI":"10.1007\/s11356-011-0694-y","article-title":"Removal of hexavalent chromium of contaminated soil by coupling electrokinetic remediation and permeable reactive biobarriers","volume":"19","author":"Fonseca","year":"2012","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1186\/s12934-016-0603-7","article-title":"Current knowledge and perspectives of Paenibacillus: a review","volume":"15","author":"Grady","year":"2016","journal-title":"Microb. Cell Factories"},{"key":"ref13","doi-asserted-by":"publisher","first-page":"1152","DOI":"10.1016\/j.cjche.2017.10.012","article-title":"Enhanced electrokinetic remediation of cadmium-contaminated natural clay using organophosphonates in comparison with EDTA","volume":"26","author":"Gu","year":"2018","journal-title":"Chin. J. Chem. Eng."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"412","DOI":"10.1016\/j.chemosphere.2015.12.087","article-title":"Effect of soil washing with only chelators or combining with ferric chloride on soil heavy metal removal and phytoavailability: field experiments","volume":"147","author":"Guo","year":"2016","journal-title":"Chemosphere"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"454","DOI":"10.15625\/0866-7160\/v35n4.3775","article-title":"Biosurfactant-producing Rhodococcus ruber TD2 isolated from oil polluted water in Vung Tau coastal zone","volume":"35","author":"Hien","year":"2013","journal-title":"Acad. J. Biol."},{"key":"ref16","doi-asserted-by":"publisher","first-page":"985","DOI":"10.1016\/j.chemosphere.2005.03.066","article-title":"Factors affecting biosurfactant production by oil degrading Aeromonas spp. isolated from a tropical environment","volume":"61","author":"Ilori","year":"2005","journal-title":"Chemosphere"},{"key":"ref17","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1016\/S0065-2911(08)00003-9","article-title":"Carbon, iron and sulfur metabolism in acidophilic microorganisms","volume":"54","author":"Johnson","year":"2009","journal-title":"Adv. Microb. Physiol."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"2389","DOI":"10.1016\/j.biortech.2009.11.023","article-title":"Optimization and characterization of a new lipopeptide biosurfactant produced by marine Brevibacterium aureum MSA13 in solid state culture","volume":"101","author":"Kiran","year":"2010","journal-title":"Bioresour. Technol."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1016\/j.jhazmat.2005.01.018","article-title":"Bioremediation of Cr (VI) in contaminated soils","volume":"121","author":"Krishna","year":"2005","journal-title":"J. Hazard. Mater."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"1472","DOI":"10.3390\/antiox10091472","article-title":"Microbial biosurfactant: a new frontier for sustainable agricultural and pharmaceutical industries","volume":"10","author":"Kumar","year":"2021","journal-title":"Antioxidants"},{"key":"ref21","doi-asserted-by":"publisher","first-page":"1701","DOI":"10.1534\/genetics.166.4.1701","article-title":"Role of the nuclease activity of Saccharomyces cerevisiae Mre 11 in repair of DNA double-strand breaks in mitotic cells","volume":"166","author":"Lewis","year":"2004","journal-title":"Genetics"},{"key":"ref22","doi-asserted-by":"publisher","first-page":"1764","DOI":"10.4238\/2014.March.17.4","article-title":"Combined folate gene MTHFD and TC polymorphisms as maternal risk factors for down syndrome in China","volume":"13","author":"Liao","year":"2014","journal-title":"Genet. Mol. Res."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"136","DOI":"10.3390\/fermentation7030136","article-title":"Glycolipid biosurfactant production from waste cooking oils by yeast: review of substrates, producers and products","volume":"7","author":"Liepins","year":"2021","journal-title":"Fermentation"},{"key":"ref24","doi-asserted-by":"publisher","first-page":"575","DOI":"10.1007\/s13762-020-02889-5","article-title":"Application of rhamnolipid surfactant for remediation of toxic metals of long and short-term contaminated sites","volume":"18","author":"Lopes","year":"2021","journal-title":"Int. J. Environ. Sci. 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Saf."},{"key":"ref006","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1007\/s10853-011-5958-x","article-title":"Nanoparticles of IrO 2 or Sb\u2013SnO 2 increase the performance of iridium oxide DSA electrodes","volume":"47","author":"Marshall","year":"2012","journal-title":"Journal of Materials Science"},{"key":"ref26","first-page":"15","article-title":"Bacterial degradation and bioremediation of polycyclic aromatic hydrocarbons","volume":"12","author":"Mrozik","year":"2010","journal-title":"Pol. J. Environ. Stud."},{"key":"ref27","first-page":"427","article-title":"Fate of heavy metals in a composting process","volume":"57","author":"M\u00fcller","year":"2012","journal-title":"Ann. Microbiol."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1016\/S0304-3894(01)00226-6","article-title":"An evaluation of technologies for the heavy metal remediation of dredged sediments","volume":"85","author":"Mulligan","year":"2001","journal-title":"J. Hazard. Mater."},{"key":"ref29","first-page":"24","article-title":"A study on the physicochemical characteristics of tannery effluent collected from Chennai","volume":"5","author":"Muthukkauppan","year":"2018","journal-title":"Int. Res. J. Eng. Technol."},{"key":"ref011","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s13205-017-1071-4","article-title":"Control of corrosive bacterial community by bronopol in industrial water system","volume":"8","author":"Narenkumar","year":"2018","journal-title":"3 Biotech"},{"key":"ref001","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1016\/S0723-2020(11)80265-1","article-title":"Survey of antimicrobial susceptibility of moderately halophilic eubacteria and extremely halophilic aerobic archaeobacteria: utilization of antimicrobial resistance as a genetic marker","volume":"16","author":"Nieto","year":"1993","journal-title":"Systematic and applied microbiology"},{"key":"ref30","article-title":"Halomonadaceae","volume-title":"Bergey\u2019s manual of systematics of archaea and bacteria","author":"Nieto","year":"2000"},{"key":"ref31","doi-asserted-by":"publisher","first-page":"193","DOI":"10.3389\/fmicb.2017.00193","article-title":"Biosurfactant and degradative enzymes mediated crude oil degradation by bacterium Bacillus subtilis A1","volume":"8","author":"Parthipan","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref32","doi-asserted-by":"publisher","first-page":"633","DOI":"10.1016\/j.jhazmat.2008.04.061","article-title":"The remediation of heavy metals contaminated sediment","volume":"161","author":"Peng","year":"2009","journal-title":"J. Hazard. Mater."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"125133","DOI":"10.1016\/j.jhazmat.2021.125133","article-title":"Metagenomic analysis of microbial community and its role in bioelectrokinetic remediation of tannery contaminated soil","volume":"412","author":"Prakash","year":"2021","journal-title":"J. Hazard. Mater."},{"key":"ref007","volume-title":"Hydrothermal Green Synthesis of Aloe Vera Gel-Biotemplated Iron Oxide Nanoparticles for Robust Photocatalytic Degradation of","author":"Priyadharsan","year":"2024"},{"key":"ref34","first-page":"55","article-title":"Bioremediation of heavy metals from contaminated soil using Pseudomonas species","volume":"58","author":"Raja","year":"2020","journal-title":"Indian J. Exp. Biol."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"222","DOI":"10.3389\/fmicb.2020.00222","article-title":"Revealing the efficacy of thermostable biosurfactant in heavy metal bioremediation and surface treatment in vegetables","volume":"11","author":"Ravindran","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1016\/j.cej.2014.02.107","article-title":"Enhanced electrokinetic technologies with oxidization-reduction for organically-contaminated soil remediation","volume":"217","author":"Ren","year":"2014","journal-title":"Chem. Eng. J."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/j.colsurfb.2006.08.009","article-title":"Isolation and partial characterization of a biosurfactant produced by Streptococcus thermophilus A","volume":"53","author":"Rodrigues","year":"2006","journal-title":"Colloids Surf. B"},{"key":"ref009","doi-asserted-by":"publisher","first-page":"141963","DOI":"10.1016\/j.chemosphere.2024.141963","article-title":"Metal organic framework anchored onto biowaste mediated carbon material (rGO) for remediation of chromium (VI) by the photocatalytic process","volume":"357","author":"Sathish","year":"2024","journal-title":"Chemosphere"},{"key":"ref014","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.chemosphere.2017.05.087","article-title":"Electrochemical decolorization of methyl red by RuO2-IrO2-TiO2 electrode and biodegradation with Pseudomonas stutzeri MN1 and Acinetobacter baumannii MN3: An integrated approach","volume":"183","author":"Sathishkumar","year":"2017","journal-title":"Chemosphere"},{"key":"ref38","doi-asserted-by":"publisher","first-page":"105262","DOI":"10.1016\/j.jtice.2023.105262","article-title":"Remediation of azodye contaminated soil by electrokinetics","author":"Santhosh","year":"2024","journal-title":"J. Taiwan Inst. Chem. Eng."},{"key":"ref013","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1007\/s40098-019-00366-6","article-title":"Electrokinetic (EK) and bio-electrokinetic (BEK) remediation of hexavalent chromium in contaminated soil using alkalophilic bio-anolyte","volume":"50","author":"Sarankumar","year":"2020","journal-title":"Indian Geotechnical Journal"},{"key":"ref39","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1007\/s12210-023-01159-z","article-title":"Combined effects of high voltage gradient and electrolyte conditioning on electrokinetic remediation for chromium (VI)-contaminated soils","volume":"34","author":"Taneja","year":"2023","journal-title":"Rend. Fis. Acc. Lincei"},{"key":"ref40","doi-asserted-by":"publisher","first-page":"2579","DOI":"10.1016\/j.cej.2017.12.010","article-title":"Biosurfactants enhanced heavy metals removal from sludge in the electrokinetic treatment","volume":"334","author":"Tang","year":"2018","journal-title":"Chem. Eng. J."},{"key":"ref41","volume-title":"Metal detoxification in eukaryotic cells","author":"Thiele","year":"1995"},{"key":"ref010","doi-asserted-by":"publisher","first-page":"104815","DOI":"10.1016\/j.jwpe.2024.104815","article-title":"Removal of hexavalent chromium from electroplating wastewater by ion-exchange in presence of Ni(II) and Zn(II) ions","volume":"58","author":"Thiripelu","year":"2024","journal-title":"J. Water Process Eng."},{"key":"ref42","doi-asserted-by":"publisher","first-page":"114239","DOI":"10.1016\/j.envres.2022.114239","article-title":"Potential mechanisms contributing to the high cadmium removal efficiency from contaminated soil by using effective microorganisms as novel electrolyte in electrokinetic remediation applications","volume":"215","author":"Tsui","year":"2022","journal-title":"Environ. Res."},{"key":"ref43","doi-asserted-by":"publisher","first-page":"122461","DOI":"10.1016\/j.envpol.2023.122461","article-title":"Long-term stability and toxicity effects of three-dimensional electrokinetic remediation on chromium-contaminated soils","volume":"337","author":"Wang","year":"2023","journal-title":"Environ. Pollut."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"54797","DOI":"10.1039\/C7RA10913J","article-title":"Electro-kinetic remediation of chromium-contaminated soil by a three-dimensional electrode coupled with a permeable reactive barrier","volume":"7","author":"Xue","year":"2017","journal-title":"RSC Adv."},{"key":"ref45","doi-asserted-by":"publisher","first-page":"140613","DOI":"10.1016\/j.cej.2022.140613","article-title":"Chromium removal from contaminated soil using a novel FeOx\/granular activated carbon-based three-dimensional electrokinetic system","volume":"455","author":"Yan","year":"2023","journal-title":"Chem. Eng. J."},{"key":"ref003","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.jhazmat.2011.08.047","article-title":"A review on techniques to enhance electrochemical remediation of contaminated soils","volume":"195","author":"Yeung","year":"2011","journal-title":"Journal of hazardous materials"},{"key":"ref004","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11270-016-2879-y","article-title":"Assessment of acid enhancement schemes for electrokinetic remediation of Cd\/Pb contaminated soil","volume":"227","author":"Zhang","year":"2016","journal-title":"Water, Air, & Soil Pollution"},{"key":"ref46","first-page":"295","article-title":"Chromium (VI) reduction by Ochrobactrum anthropi: mechanisms and implications for bioremediation","volume":"173","author":"Zhu","year":"2017","journal-title":"Chemosphere"}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2024.1458369\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T00:44:37Z","timestamp":1727138677000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2024.1458369\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,24]]},"references-count":60,"alternative-id":["10.3389\/fmicb.2024.1458369"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2024.1458369","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,24]]},"article-number":"1458369"}}