{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T20:47:17Z","timestamp":1791492437629,"version":"4.3.4"},"reference-count":73,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T00:00:00Z","timestamp":1728950400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Klebsiella quasipneumoniae<\/jats:italic> (previously known as <jats:italic>K. pneumoniae<\/jats:italic> K6) strains are among the multidrug-resistant hypervirulent bacterial pathogens. Phage therapy can help treat infections caused by such pathogens. Here we report some aspects of virology and therapeutic potentials of vB_KquU_\u03c6KuK6, a bacteriophage that infects <jats:italic>Klebsiella quasipneumoniae.<\/jats:italic><\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:italic>K. quasipneumoniae<\/jats:italic> (ATCC 700603) was used to screen wastewater lytic phages. The isolate vB_KquU_\u03c6KuK6 that consistently created large clear plaques was characterized using standard virological and molecular methods.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>vB_KquU_\u03c6KuK6 has a complex capsid with an icosahedral head (~60\u2009nm) and a slender tail (~140\u2009nm \u00d7 10\u2009nm). The phage has a 51% AT-rich linear dsDNA genome (51,251\u2009bp) containing 121 open reading frames. The genome contains genes encoding spanin, endolysin, and holin proteins necessary for lytic infection and a recombinase gene possibly involved in lysogenic infection. vB_KquU_\u03c6KuK6 is stable at \u221280 to +67\u00b0C, pH 4\u20139, and brief exposure to one volume percent of chloroform. vB_KquU_\u03c6KuK6 has a narrow host range. Its lytic infection cycle involves a latency of 20\u2009min and a burst size of 435 plaque-forming units. The phage can cause lysogenic infection, and the resulting lysogens are resistant to lytic infection by vB_KquU_\u03c6KuK6. vB_KquU_\u03c6KuK6 reduces the host cells\u2019 ability to form biofilm but fails to eliminate that ability. vB_KquU_\u03c6KuK6 demonstrates phage-antibiotic synergy and reduces the minimum inhibitory concentration of chloramphenicol and neomycin sulfate by about 8 folds.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>vB_KquU_\u03c6KuK6 cannot be directly used for phage therapy because it is a temperate bacteriophage. However, genetically modified strains of vB_KquU_\u03c6KuK6 alone or combined with antibiotics or other lytic <jats:italic>Klebsiella<\/jats:italic> phages can have therapeutic utilities in treating <jats:italic>K. quasipneumoniae<\/jats:italic> infections.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2024.1472729","type":"journal-article","created":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T13:40:19Z","timestamp":1728999619000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Isolation and preliminary characterization of a novel bacteriophage vB_KquU_\u03c6KuK6 that infects the multidrug-resistant pathogen Klebsiella quasipneumoniae"],"prefix":"10.3389","volume":"15","author":[{"given":"Isaac P.","family":"Miller","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alma G.","family":"Laney","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geoffrey","family":"Zahn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brock J.","family":"Sheehan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiara V.","family":"Whitley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruhul H.","family":"Kuddus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,10,15]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1019","DOI":"10.3390\/ph14101019","article-title":"Phage cocktail development for bacteriophage therapy: toward improving spectrum of activity breadth and depth","volume":"14","author":"Abedon","year":"2021","journal-title":"Pharmaceuticals (Basel)"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"1044143","DOI":"10.3389\/fmicb.2022.1044143","article-title":"Bacteriophage-encoded lethal membrane disruptors: Advances in understanding and potential applications","volume":"13","author":"Abeysekera","year":"2022","journal-title":"Front. 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