{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T02:26:27Z","timestamp":1786242387155,"version":"build-2736575974"},"reference-count":65,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T00:00:00Z","timestamp":1737417600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p><jats:italic>Listeria monocytogenes<\/jats:italic> are Gram-positive, non-spore-producing rods that are the etiological agent of listeriosis. <jats:italic>L. monocytogenes<\/jats:italic> is isolated from soil, water, sewage, rotting vegetation, and the main source of these rods for humans is food (fish, unpasteurized dairy products, or raw fruits and vegetables). In recent years, there has been an increase in consumer interest in healthy food, especially organically grown. The use of natural fertilizers during organic farming can be a source of pathogens, including <jats:italic>L. monocytogenes<\/jats:italic> in the soil and finally in vegetables and fruits. The aim of this study was to assess the prevalence of <jats:italic>L. monocytogenes<\/jats:italic> in soil samples from organic carrot crops (Poland) and to characterize the tested strains. Microbial contamination of the soil has a direct impact on the safety of the crops grown on it. This is an important aspect in organic farming, where products are chosen as a healthier option and consumed by children and the elderly.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The isolates were subjected to genetic similarity assessment (PFGE method), and the tested strains were evaluated for antibiotic susceptibility (disc-diffusion method), invasiveness (HT-29 line human colon cancer cell line), coaggregation with <jats:italic>Salmonella<\/jats:italic> Enteritidis, biofilm-forming ability and the effect of disinfectants on the biofilm.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Twenty-seven isolates of <jats:italic>L. monocytogenes<\/jats:italic> were isolated from 250 soil samples, 10 of which were genetically different. 80% of the tested strains were sensitive to the tested antibiotics. Antibiotic resistance was demonstrated in two strains (strain 11 \u2013 resistant to penicillin and cotrimoxazole, strain 22 \u2013 resistant to penicillin). The highest invasiveness against HT-29 cells at 23.2% was shown for strain 11. However, invasiveness of less than 1% was demonstrated for three strains, and strain 13 showed no ability to invade HT-29 human colon cancer cells. The level of coaggregation between the tested strains and <jats:italic>S.<\/jats:italic> Enteritidis ranged from 22.2 to 39.1%. The number of biofilm-isolated rods from the stainless steel surface was 6.37 to 7.10 log colony-forming unit (CFU)\/cm<jats:sup>2<\/jats:sup>, while on polypropylene it was from 6.75 to 8.06 log CFU\/cm<jats:sup>2<\/jats:sup>. The effectiveness of the disinfectants used depended on the duration of action and the concentration of the disinfectant. Chlorosol was shown to be the disinfectant causing stronger biofilm eradication on each of the tested surfaces. It has been shown that soils and thus food from organic farming can be a source of <jats:italic>L. monocytogenes<\/jats:italic>. These rods can vary in phenotypic characteristics and virulence levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>The research conducted allows to draw attention to the occurrence of pathogens, including <jats:italic>L. monocytogenes<\/jats:italic> in crops from organic farming. In addition, the results presented can help to introduce standards regulating the safety of organic farming, taking into account the occurrence of antibiotic-resistant or highly invasive strains, thus maintaining food safety.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2024.1530446","type":"journal-article","created":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T08:37:21Z","timestamp":1737448641000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Characterization of Listeria monocytogenes strains isolated from soil under organic carrot farming"],"prefix":"10.3389","volume":"15","author":[{"given":"Maciej","family":"Nowak","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zbigniew","family":"Paluszak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Wiktorczyk-Kapischke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Grudlewska-Buda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Budzy\u0144ska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Skonieczna-Kurpiel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewa","family":"Wa\u0142ecka-Zacharska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Huse-Kutowska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krzysztof","family":"Skowron","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,1,21]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1016\/j.fm.2018.01.003","article-title":"Sources and contamination routes of microbial pathogens to fresh produce during field cultivation: a review","volume":"73","author":"Alegbeleye","year":"2018","journal-title":"Food Microbiol."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1111\/j.1472-765x.1996.tb01177.x","article-title":"Development of a polymerase chain reaction assay for the detection of Listeria monocytogenes in foods","volume":"22","author":"Bansal","year":"1996","journal-title":"Lett. Appl. Microbiol."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1007\/s00003-014-0923-7","article-title":"Adhesion of Listeria monocytogenes to cutting board surfaces and removal by different sanitizers","volume":"10","author":"Beltrame","year":"2015","journal-title":"J. Consum. Protect. Food Saf."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"2385","DOI":"10.1111\/j.1462-2920.2010.02297.x","article-title":"Fresh fruit and vegetables as vehicles for the transmission of human pathogens","volume":"12","author":"Berger","year":"2010","journal-title":"Environ. Microbiol."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"158","DOI":"10.1111\/j.1472-765x.1990.tb00149.x","article-title":"Detection of Listeria species and Listeria monocytogenes using polymerase chain reaction","volume":"11","author":"Border","year":"1990","journal-title":"Lett. Appl. Microbiol."},{"key":"ref7","article-title":"Listeria monocytogenes, biofilm formation and fresh cut produce","volume-title":"Microbial pathogens and strategies for combating them: Science, technology and education","author":"Botticella","year":"2013"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1046\/j.1472-765x.2002.01198.x","article-title":"Inactivation of Listeria monocytogenes\/Flavobacterium spp. biofilms using chlorine: impact of substrate, pH, time and concentration","volume":"35","author":"Bremer","year":"2002","journal-title":"Lett. Appl. Microbiol."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"994","DOI":"10.4315\/0362-028X-62.9.994","article-title":"Effects of the growth procedure on the surface hydrophobicity of Listeria monocytogenes cells and their adhesion to stainless steel","volume":"62","author":"Briandet","year":"1999","journal-title":"J. Food Prot."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.ijfoodmicro.2015.04.026","article-title":"Control of pathogens in biofilms on the surface of stainless steel by levulinic acid plus sodium dodecyl sulfate","volume":"207","author":"Chen","year":"2015","journal-title":"Int. J. Food Microbiol."},{"key":"ref2","volume-title":"Commission regulation (EU) 2024\/2895 of 20 November 2024 amending regulation (EC) no 2073\/2005 as regards","year":"2024"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"M89","DOI":"10.1111\/j.1750-3841.2007.00294.x","article-title":"Indigenous dadih lactic acid bacteria: cell-surface properties and interactions with pathogens","volume":"72","author":"Collado","year":"2007","journal-title":"J. Food Sci."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1590\/S1517-83822010000100016","article-title":"Biofilm formation by Listeria monocytogenes on stainless steel surface and biotransfer potential","volume":"41","author":"de Oliveira","year":"2010","journal-title":"Braz. J. Microbiol."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"e0137046","DOI":"10.1371\/journal.pone.0137046","article-title":"Biofilm-forming abilities of Listeria monocytogenes serotypes isolated from different sources","volume":"10","author":"Doijad","year":"2015","journal-title":"PLoS One"},{"key":"ref14","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.geoderma.2017.02.025","article-title":"Metabolic and genetic patterns of soil microbial communities in response to different amendments under organic farming system","volume":"296","author":"Dumontet","year":"2017","journal-title":"Geoderma"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"520","DOI":"10.4315\/0362-028X-51.7.520","article-title":"Inactivation of Listeria Monocytogenes by chlorine","volume":"51","author":"El-Kest","year":"1988","journal-title":"J. Food Prot."},{"key":"ref16","volume-title":"Breakpoints tables for interpretation of MICs and zones diameters. Version 12.0","year":""},{"key":"ref17","doi-asserted-by":"publisher","first-page":"e8442","DOI":"10.2903\/j.efsa.2023.8442","article-title":"The European Union one health 2022 Zoonoses report","volume":"21","year":"2023","journal-title":"EFSA J."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"1292","DOI":"10.4315\/0362-028x-69.6.1292","article-title":"Chlorine resistance of Listeria monocytogenes biofilms and relationship to subtype, cell density, and planktonic cell chlorine resistance","volume":"69","author":"Folsom","year":"2006","journal-title":"J. Food Prot."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"250","DOI":"10.1016\/j.ijfoodmicro.2010.12.031","article-title":"Salmonella Typhimurium internalization is variable in leafy vegetables and fresh herbs","volume":"145","author":"Golberg","year":"2011","journal-title":"Int. J. Food Microbiol."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"863","DOI":"10.3389\/fmicb.2016.00863","article-title":"Use of potential probiotic lactic acid Bacteria (LAB) biofilms for the control of Listeria monocytogenes, salmonella Typhimurium, and Escherichia coli O157:H7 biofilms formation","volume":"7","author":"G\u00f3mez","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"2488","DOI":"10.4315\/0362-028x-67.11.2488","article-title":"Strain-specific differences in the attachment of Listeria monocytogenes to alfalfa sprouts","volume":"67","author":"Gorski","year":"2004","journal-title":"J. Food Prot."},{"key":"ref9001","doi-asserted-by":"publisher","first-page":"1165","DOI":"10.1016\/j.foodcont.2006.06.014","article-title":"Influence of food soiling matrix on cleaning and disinfection efficiency on surface attached Listeria monocytogenes","volume":"18","author":"Gram","year":"2007","journal-title":"Food Control."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"303","DOI":"10.1016\/j.acthis.2018.03.007","article-title":"Current methodology of MTT assay in bacteria - A review","volume":"120","author":"Grela","year":"2018","journal-title":"Acta Histochem."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"1958","DOI":"10.4315\/JFP-20-120","article-title":"Listeria monocytogenes prevalence varies more within fields than between fields or over time on conventionally farmed New York produce fields","volume":"83","author":"Harrand","year":"2020","journal-title":"J. Food Prot."},{"key":"ref24","doi-asserted-by":"publisher","first-page":"613","DOI":"10.1111\/j.1365-2672.2007.03587.x","article-title":"Microbial contamination of fruit and vegetables and the behaviour of enteropathogens in the phyllosphere: a review","volume":"104","author":"Heaton","year":"2008","journal-title":"J. Appl. Microbiol."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1089\/153531404772914437","article-title":"Persistence of Salmonella enterica serovar typhimurium on lettuce and parsley and in soils on which they were grown in fields treated with contaminated manure composts or irrigation water","volume":"1","author":"Islam","year":"2004","journal-title":"Foodborne Pathog. Dis."},{"key":"ref26","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1186\/s12866-015-0476-7","article-title":"Prevalence, antimicrobial susceptibility and virulotyping of Listeria species and Listeria monocytogenes isolated from open-air fish markets","volume":"15","author":"Jamali","year":"2015","journal-title":"BMC Microbiol."},{"key":"ref27","first-page":"19","article-title":"Aggregation ability of potential probiotic Lactobacillus plantarum strains","volume":"6","author":"Jankovi\u0107","year":"2012","journal-title":"Int. J. Sanit. Eng. Res."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"3640","DOI":"10.1128\/AEM.69.6.3640-3645.2003","article-title":"Adhesion, invasion, and translocation characteristics of Listeria monocytogenes serotypes in Caco-2 cell and mouse models","volume":"69","author":"Jaradat","year":"2003","journal-title":"Appl. Environ. Microbiol."},{"key":"ref29","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1002\/9781444319347.ch8","article-title":"The role of manure and compost in produce safety","volume-title":"Microbial safety of fresh produce","author":"Jiang","year":"2009"},{"key":"ref30","first-page":"21","article-title":"Antibiotic resistance profile of L. monocytogenes isolated from food sources","volume":"2","author":"Khan","year":"2014","journal-title":"Indian J. Biotechnol. Pharmaceutical Res."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"254","DOI":"10.1016\/0076-6879(94)36020-0","article-title":"Coaggregation between bacterial species","volume":"236","author":"Kinder","year":"1994","journal-title":"Methods Enzymol."},{"key":"ref32","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/j.micpath.2018.04.034","article-title":"Listeria monocytogenes - danger for health safety vegetable production","volume":"120","author":"Kljujev","year":"2018","journal-title":"Microb. Pathog."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"246","DOI":"10.4315\/0362-028X-55.4.246","article-title":"Effect of cleaners and sanitizers on Listeria monocytogenes attached to product contact surfaces","volume":"55","author":"Krysinski","year":"1992","journal-title":"J. Food Prot."},{"key":"ref34","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1016\/j.carbpol.2015.01.078","article-title":"Effect of sodium-alginate and laminaran on Salmonella Typhimurium infection in human enterocyte-like HT-29-Luc cells and BALB\/c mice","volume":"125","author":"Kuda","year":"2015","journal-title":"Carbohydr. Polym."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1016\/j.bjm.2016.10.006","article-title":"Microbiology of organic and conventionally grown fresh produce. Braz","volume":"47","author":"Maffei","year":"2016","journal-title":"J. Microbiol."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"226","DOI":"10.1016\/j.foodcont.2012.06.013","article-title":"Microbial quality of organic and conventional vegetables soil in Brazil","volume":"29","author":"Maffei","year":"2013","journal-title":"Food Control"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1002\/9781444319347.ch1","article-title":"Enteric human pathogens associated with fresh produce: sources, transport, and ecology","volume-title":"Microbial safety of fresh produce","author":"Mandrell","year":"2009"},{"key":"ref38","doi-asserted-by":"publisher","first-page":"679","DOI":"10.1016\/j.fm.2010.03.008","article-title":"Microbiological quality of fresh lettuce from organic and conventional production","volume":"27","author":"Oliveira","year":"2010","journal-title":"Food Microbiol."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"7711","DOI":"10.1128\/AEM.01065-06","article-title":"Resistance of Listeria monocytogenes biofilms to sanitizing agents in a simulated food processing environment","volume":"72","author":"Pan","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1016\/j.ijfoodmicro.2016.08.029","article-title":"Variability of Listeria monocytogenes strains in biofilm formation on stainless steel and polystyrene materials and resistance to peracetic acid and quaternary ammonium compounds","volume":"237","author":"Poimenidou","year":"2016","journal-title":"Int. J. Food Microbiol."},{"key":"ref42","volume-title":"Chemiczne \u015brodki dezynfekcyjne i antyseptyczne - Ilo\u015bciowa zawiesinowa metoda okre\u015blania dzia\u0142ania bakteriob\u00f3jczego chemicznych \u015brodk\u00f3w dezynfekcyjnych i antyseptycznych stosowanych w sektorze \u017cywno\u015bciowym, warunkach przemys\u0142owych i domowych oraz zak\u0142adach u\u017cyteczno\u015bci publicznej -- Metoda badania i wymagania (faza 2, etap 1)","year":"2010"},{"key":"ref43","year":"2013"},{"key":"ref44","year":"2018"},{"key":"ref45","doi-asserted-by":"publisher","first-page":"587","DOI":"10.1016\/j.bjm.2017.01.004","article-title":"Growth, viability and architecture of biofilms of Listeria monocytogenes formed on abiotic surfaces","volume":"48","author":"Reis-Teixeira","year":"2017","journal-title":"Braz. J. Microbiol."},{"key":"ref46","doi-asserted-by":"publisher","first-page":"750","DOI":"10.4315\/0362-028X-56.9.750","article-title":"(1993). Biofilm development and sanitizer inactivation of Listeria monocytogenes and Salmonella typhimurium on stainless-steel and Buna-N rubber","volume":"56","author":"Ronner","year":"1993","journal-title":"J. Food Prot."},{"key":"ref47","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1002\/9781444319347.ch3","article-title":"Internalization of pathogens in produce","volume-title":"Microbial safety of fresh produce","author":"Ryser","year":"2009"},{"key":"ref48","volume-title":"Organic agriculture, environment and food security","author":"Scialabba","year":"2002"},{"key":"ref49","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.foodpol.2016.12.009","article-title":"What is this thing called organic? \u2013 how organic farming is codified in regulations","volume":"68","author":"Seufert","year":"2017","journal-title":"Food Policy"},{"key":"ref9004","doi-asserted-by":"publisher","first-page":"53","DOI":"10.2174\/1874285801711010053","article-title":"Understanding the Mechanism of Bacterial Biofilms Resistance to Antimicrobial Agents","volume":"11","author":"Singh","year":"2017","journal-title":"Open Microbiol J."},{"key":"ref50","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1007\/s10123-018-0002-5","article-title":"Comparison of selected disinfectants efficiency against Listeria monocytogenes biofilm formed on various surfaces","volume":"21","author":"Skoworn","year":"2018","journal-title":"Int. Microbiol."},{"key":"ref51","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1007\/s13213-018-1432-1","article-title":"Phenotypic and genotypic evaluation of Listeria monocytogenes strains isolated from fish and fish processing plants","volume":"69","author":"Skowron","year":"2019","journal-title":"Ann. Microbiol."},{"key":"ref52","doi-asserted-by":"publisher","first-page":"12475","DOI":"10.5897\/AJB11.3939","article-title":"Adhesive ability and biofilm metabolic activity of Listeria monocytogenes strains before and after cold stress","volume":"11","author":"Slama","year":"2012","journal-title":"Afr. J. Biotechnol."},{"key":"ref53","first-page":"2169","article-title":"Antagonistic activity of probiotic lactobacilli against Staphylococcus aureus isolated from bovine mastitis","volume":"44","author":"Soleimani","year":"2010","journal-title":"Afr J Microbiol."},{"key":"ref54","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1016\/j.ijfoodmicro.2018.08.018","article-title":"Microbial quality of organic and conventional vegetables from polish farms","volume":"286","author":"Szczech","year":"2018","journal-title":"Int. J. Food Microbiol."},{"key":"ref55","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1007\/s12223-013-0260-8","article-title":"Anthropogenic impact on the presence of L. monocytogenes in soil, fruits, and vegetables","volume":"59","author":"Szymczak","year":"2014","journal-title":"Folia Microbiol. (Praha)"},{"key":"ref56","doi-asserted-by":"publisher","first-page":"1411","DOI":"10.4315\/0362-028X.JFP-13-514","article-title":"Bacteriological quality of vegetables from organic and conventional production in different areas of Korea","volume":"77","author":"Tango","year":"2014","journal-title":"J. Food Prot."},{"key":"ref57","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1046\/j.1365-2672.1998.00515.x","article-title":"Tissue culture assays using Caco-2 cell line differentiate virulent from non-virulent Listeria monocytogenes strains","volume":"85","author":"Van Langendonck","year":"1998","journal-title":"J. Appl. Microbiol."},{"key":"ref58","doi-asserted-by":"publisher","first-page":"317","DOI":"10.3389\/fmicb.2014.00317","article-title":"Life on the outside: role of biofilms in environmental persistence of Shiga-toxin producing Escherichia coli","volume":"5","author":"Vogeleer","year":"2014","journal-title":"Front. Microbiol."},{"key":"ref59","doi-asserted-by":"publisher","first-page":"216","DOI":"10.1111\/lam.12036","article-title":"Salt stress-induced invasiveness of major Listeria monocytogenes serotypes","volume":"56","author":"Wa\u0142ecka-Zacharska","year":"2013","journal-title":"Lett. Appl. Microbiol."},{"key":"ref60","doi-asserted-by":"publisher","first-page":"797","DOI":"10.1128\/AEM.03088-15","article-title":"Validation of a previously developed geospatial model that predicts the prevalence of Listeria monocytogenes in New York state produce fields","volume":"82","author":"Weller","year":"2016","journal-title":"Appl. Environ. Microbiol."},{"key":"ref61","doi-asserted-by":"publisher","first-page":"1161486","DOI":"10.3389\/fmolb.2023.1161486","article-title":"Genomic and pathogenicity islands of Listeria monocytogenes-overview of selected aspects","volume":"10","author":"Wiktorczyk-Kapischke","year":"2023","journal-title":"Front. Mol. Biosci."},{"key":"ref62","doi-asserted-by":"publisher","first-page":"1492","DOI":"10.3390\/foods11101492","article-title":"Comparison of selected phenotypic features of persistent and sporadic strains of Listeria monocytogenes sampled from fish processing plants","volume":"11","author":"Wiktorczyk-Kapischke","year":"2022","journal-title":"Food Secur."},{"key":"ref63","year":"2018"},{"key":"ref64","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/B978-0-12-404611-5.00002-6","article-title":"Microbial attachment and persistence on plants","volume-title":"The produce contamination problem. Causes and solutions","author":"Yaron","year":"2014"}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2024.1530446\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T08:37:27Z","timestamp":1737448647000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2024.1530446\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,21]]},"references-count":65,"alternative-id":["10.3389\/fmicb.2024.1530446"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2024.1530446","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,21]]},"article-number":"1530446"}}