{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,3]],"date-time":"2026-01-03T06:47:09Z","timestamp":1767422829066,"version":"3.41.2"},"reference-count":38,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,3,21]],"date-time":"2025-03-21T00:00:00Z","timestamp":1742515200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>A single nucleotide polymorphism (SNP) in the 126 bp untranslated region (UTR) directly upstream of <jats:italic>Campylobacter jejuni\u2019s cysM<\/jats:italic> (cysteine synthase) results in significant effects on gene transcription. UTR sequences, containing the predicted promoter region of <jats:italic>cysM<\/jats:italic>, from 264 different strains were compared, and revealed a SNP twenty nucleotides upstream of the <jats:italic>cysM<\/jats:italic> translation start site. In 219 strains the UTR sequence contained a guanine at this locus, and the remaining 45 strains had an adenine at the same position. Strains possessing the guanine SNP showed higher amounts of <jats:italic>cysM<\/jats:italic> transcripts compared to adenine SNP strains. When both UTR regions were cloned upstream of the major flagellar subunit (<jats:italic>flaA<\/jats:italic>) the guanine SNP UTR resulted in significantly greater levels of <jats:italic>flaA<\/jats:italic> transcription compared to the adenine SNP containing UTR. Additionally, when the UTR containing the guanine SNP was fused to <jats:italic>flaA<\/jats:italic>, motility was restored for a <jats:italic>flaAB<\/jats:italic> null mutant. Motility was not rescued initially when <jats:italic>flaA<\/jats:italic> was fused to the UTR containing the adenine SNP UTR. However, when the <jats:italic>flaAB<\/jats:italic> null mutant, containing a copy of <jats:italic>flaA<\/jats:italic> fused to the adenine-containing UTR, was incubated in Brucella broth for a minimum of two consecutive passages each lasting 48 h, transcription of <jats:italic>flaA<\/jats:italic> increased and motility was restored. Additional analysis of the <jats:italic>flaA<\/jats:italic> mRNA produced by the strain containing the adenine SNP UTR fused to <jats:italic>flaA<\/jats:italic> grown in Brucella broth versus agar suggests that the effects on motility occurred through blocking of full-length mRNA production.<\/jats:p>","DOI":"10.3389\/fmicb.2025.1501331","type":"journal-article","created":{"date-parts":[[2025,3,21]],"date-time":"2025-03-21T08:19:56Z","timestamp":1742545196000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["A single nucleotide polymorphism produces different transcription profiles in Campylobacter jejuni\u2019s cysM"],"prefix":"10.3389","volume":"16","author":[{"given":"Nereus W.","family":"Gunther IV","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siddhartha","family":"Kanrar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aisha","family":"Abdul-Wakeel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"McAnulty","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Renye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph","family":"Uknalis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gaylen A.","family":"Uhlich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,3,21]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1016\/j.resmic.2010.09.024","article-title":"MCLMAN, a new minimal medium for Campylobacter jejuni NCTC 11168.","volume":"162","author":"Alazzam","year":"2011","journal-title":"Res. 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