{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T03:07:24Z","timestamp":1792552044815,"version":"4.5.0"},"reference-count":48,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,4,30]],"date-time":"2025-04-30T00:00:00Z","timestamp":1745971200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Di-n-butyl phthalate (DBP) is one of the most widely used phthalate esters (PAEs) and is considered an emerging global pollutant. It may pose a significant threat to ecosystem and human health due to its residual hazards and accumulation in the environment. Bacteria-driven PAE biodegradation is considered an economical and effective strategy for remediating such polluted environments.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A DBP-degrading bacterium (P-7), was isolated from long-term film mulched cotton field soil. Its identity was confirmed via physiological, biochemical, and 16S rRNA gene analyses. The degradation conditions were optimized through single-factor experiments and response surface methodology (RSM).Furthermore, the whole-genome sequencing coupled with metabolomics was employed to elucidate metabolic mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>Priestia megaterium<\/jats:italic> P-7 (<jats:italic>P. megaterium<\/jats:italic> P-7) achieved 100% DBP removal within 20 h under optimal conditions and exhibited broad substrate specificity for other PAEs. Genomic analysis identified key genes (<jats:italic>lip<\/jats:italic>, <jats:italic>aes<\/jats:italic>, <jats:italic>ybfF<\/jats:italic>, <jats:italic>estA<\/jats:italic>, and <jats:italic>yvaK<\/jats:italic>) encoding esterases\/hydrolases that initiate DBP catabolism, converting it to phthalic acid (PA). Subsequent decarboxylation (<jats:italic>pdc<\/jats:italic>, <jats:italic>bsdCD<\/jats:italic>, <jats:italic>mdcACDH<\/jats:italic>, and <jats:italic>lysA<\/jats:italic>) and dioxygenase-mediated steps integrated PA into the TCA cycle. Metabolomics revealed three degradation pathways: decarboxylation (DBP\u202f\u2192\u202fMBP\u202f\u2192\u202fBB\u202f\u2192\u202fBA\u2192Catechol), hydrolysis (DBP\u202f\u2192\u202fMBP\u202f\u2192\u202fPA\u202f\u2192\u202fPCA\u202f\u2192\u202fCatechol) and direct \u03b2-oxidation (DBP\u202f\u2192\u202fDEP\u202f\u2192\u202fMEP\u202f\u2192\u202fPA\u202f\u2192\u202fCatechol).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:italic>P. megaterium<\/jats:italic> P-7 demonstrates exceptional degradation efficiency, substrate versatility, and environmental stress tolerance, making it a promising candidate for bioremediation of organic pollutants in contaminated soil.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2025.1538746","type":"journal-article","created":{"date-parts":[[2025,4,30]],"date-time":"2025-04-30T05:39:44Z","timestamp":1745991584000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Screening, identification, metabolic pathway of di-n-butyl phthalate degrading Priestia megaterium P-7 isolated from long-term film mulched cotton field soil in Xinjiang"],"prefix":"10.3389","volume":"16","author":[{"given":"Yuanyang","family":"Yi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanqin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiying","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiong","family":"Jia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Munire","family":"Mutalifu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhidong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,4,30]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"127481","DOI":"10.1016\/j.micres.2023.127481","article-title":"Whole genome sequencing analysis and box-Behnken design for the optimization of the decolourization of mixture textile dyes by halotolerant microbial consortium","volume":"276","author":"Alghamdi","year":"2023","journal-title":"Microbiol. 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