{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T22:46:31Z","timestamp":1792104391510,"version":"4.4.0"},"reference-count":71,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T00:00:00Z","timestamp":1738713600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>This study aimed to characterize antibiotic-resistance plasmids present in microorganisms from sprout samples using exogenous plasmid capture. Fresh mung bean sprouts were predominantly colonized by bacteria from the phyla <jats:italic>Proteobacteria<\/jats:italic> and <jats:italic>Bacteroidetes<\/jats:italic>. To capture plasmids, a plasmid-free <jats:italic>Escherichia<\/jats:italic> (<jats:italic>E.<\/jats:italic>) <jats:italic>coli<\/jats:italic> CV601 strain, containing a green fluorescent protein gene for selection, was used as the recipient strain in exogenous plasmid capture experiments. Transconjugants were selected on media containing cefotaxime or tetracycline antibiotics. While no cefotaxime-resistant transconjugants were obtained, 40 tetracycline-resistant isolates were obtained and sequenced by Illumina NextSeq short read and Nanopore MinION long read sequencing. Sequences were assembled using Unicycler hybrid assembly. Most of the captured long plasmids carried either the <jats:italic>tet<\/jats:italic>(A) or <jats:italic>tet<\/jats:italic>(D) resistance gene, belonged to the IncFI or IncFII replicon types, and were predicted as conjugative. While the smaller plasmids contained the <jats:italic>tet<\/jats:italic>(A) tetracycline resistance gene as well as additional quinolone (<jats:italic>qnr<\/jats:italic>S1), sulfonamide (<jats:italic>sul<\/jats:italic>1) and trimethoprim (<jats:italic>dfr<\/jats:italic>A1) resistance genes, the larger plasmids only contained the <jats:italic>tet<\/jats:italic>(D) resistance gene. An exception was the largest 192 kbp plasmid isolated, which contained the <jats:italic>tet<\/jats:italic>(D), as well as sulfonamide (<jats:italic>sul1<\/jats:italic>) and streptomycin (<jats:italic>aad<\/jats:italic>A1) resistance genes. The smaller plasmid was isolated from different sprout samples more often and showed a 100% identity in size (71,155\u202fbp), while the 180 kbp plasmids showed some smaller or larger differences (in size between 157,683 to 192,360\u202fbp). This suggested that the plasmids obtained from the similar sprout production batches could be clonally related. Nanopore MinION based 16S metagenomics showed the presence of <jats:italic>Enterobacter<\/jats:italic> (<jats:italic>En.<\/jats:italic>) <jats:italic>cloacae<\/jats:italic>, <jats:italic>En. ludwigii<\/jats:italic>, <jats:italic>En. kobei<\/jats:italic>, <jats:italic>Citrobacter<\/jats:italic> (<jats:italic>C.<\/jats:italic>) <jats:italic>werkmanii<\/jats:italic>, <jats:italic>C. freundii<\/jats:italic>, <jats:italic>Klebsiella<\/jats:italic> (<jats:italic>K.<\/jats:italic>) <jats:italic>oxytoca<\/jats:italic> and <jats:italic>K. pneumonia<\/jats:italic>, which have previously been isolated from fresh produce in Germany. These bacteria may harbor antibiotic resistance genes on plasmids that could potentially be transferred to similar genera. This study demonstrated that bacteria present in sprouts may act as the donors of antibiotic resistance plasmids which can transfer resistance to other bacteria on this product via conjugation.<\/jats:p>","DOI":"10.3389\/fmicb.2025.1538973","type":"journal-article","created":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T07:38:43Z","timestamp":1738741123000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Exogenous plasmid capture to characterize tetracycline-resistance plasmids in sprouts obtained from retail in Germany"],"prefix":"10.3389","volume":"16","author":[{"given":"Maria","family":"Stein","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Brinks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Habermann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gyu-Sung","family":"Cho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles M. 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Microbiol."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1016\/S0140-6736(21)02724-0","article-title":"Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis","volume":"399","author":"Antimicrobial Resistance","year":"2022","journal-title":"Lancet"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"W16","DOI":"10.1093\/nar\/gkw387","article-title":"PHASTER: a better, faster version of the PHAST phage search tool","volume":"44","author":"Arndt","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"392","DOI":"10.1111\/jfs.12256","article-title":"Seasonal and growth-dependent dynamics of bacterial Community in Radish Sprouts","volume":"36","author":"Asakura","year":"2016","journal-title":"J. Food Saf."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.plasmid.2019.03.005","article-title":"Don't overlook the little guy: an evaluation of the frequency of small plasmids co-conjugating with larger carbapenemase gene containing plasmids","volume":"103","author":"Barry","year":"2019","journal-title":"Plasmid"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"862","DOI":"10.1038\/s41396-020-00819-4","article-title":"Plasmid- and strain-specific factors drive variation in ESBL-plasmid spread in vitro and in vivo","volume":"15","author":"Benz","year":"2021","journal-title":"ISME J."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1016\/j.fm.2017.03.005","article-title":"Microbiological quality of selected ready-to-eat leaf vegetables, sprouts and non-pasteurized fresh fruit-vegetable juices including the presence of Cronobacter spp","volume":"65","author":"Berthold-Pluta","year":"2017","journal-title":"Food Microbiol."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"e01300","DOI":"10.1128\/mBio.01300-18","article-title":"The transferable Resistome of produce","volume":"9","author":"Blau","year":"2018","journal-title":"MBio"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1007\/978-1-4939-9877-7_3","article-title":"Detection, isolation, and characterization of plasmids in the environment","volume":"2075","author":"Blau","year":"2020","journal-title":"Methods Mol. Biol."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"522","DOI":"10.1002\/mnfr.200400030","article-title":"Occurrence of antibiotic-resistant enterobacteria in agricultural foodstuffs","volume":"48","author":"Boehme","year":"2004","journal-title":"Mol. Nutr. Food Res."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1007\/s00284-023-03201-7","article-title":"Fast identification method for screening Bacteria from Faecal samples using Oxford Nanopore technologies MinION sequencing","volume":"80","author":"Borges","year":"2023","journal-title":"Curr. Microbiol."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1099\/mgen.0.000203","article-title":"Extended-spectrum beta-lactamase-encoding genes are spreading on a wide range of Escherichia coli plasmids existing prior to the use of third-generation cephalosporins","volume":"4","author":"Branger","year":"2018","journal-title":"Microb Genom"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"1763","DOI":"10.1056\/NEJMoa1106482","article-title":"German outbreak of Escherichia coli O104:H4 associated with sprouts","volume":"365","author":"Buchholz","year":"2011","journal-title":"N. Engl. J. Med."},{"key":"ref16","doi-asserted-by":"publisher","first-page":"303","DOI":"10.17590\/20200120-102303","article-title":"Escherichia coli in flour \u2013 sources, risks and prevention","volume":"20","year":"2020","journal-title":"BfR Opin."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1089\/fpd.2014.1821","article-title":"Reported foodborne outbreaks due to fresh produce in the United States and European Union: trends and causes","volume":"12","author":"Callejon","year":"2015","journal-title":"Foodborne Pathog. Dis."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"100071","DOI":"10.1016\/j.jfp.2023.100071","article-title":"Salmonella outbreaks linked to beef, United States, 2012-2019","volume":"86","author":"Canning","year":"2023","journal-title":"J. Food Prot."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1016\/j.mimet.2005.03.018","article-title":"Identification of plasmids by PCR-based replicon typing","volume":"63","author":"Carattoli","year":"2005","journal-title":"J. Microbiol. Methods"},{"key":"ref20","doi-asserted-by":"publisher","first-page":"126174","DOI":"10.1016\/j.syapm.2020.126174","article-title":"Polyphasic study of antibiotic-resistant enterobacteria isolated from fresh produce in Germany and description of Enterobacter vonholyi sp. nov. isolated from marjoram and Enterobacter dykesii sp. nov. isolated from mung bean sprout","volume":"44","author":"Cho","year":"2021","journal-title":"Syst. Appl. Microbiol."},{"key":"ref21","author":"Chris Baylis","year":"2011"},{"key":"ref22","year":"2022"},{"key":"ref23","doi-asserted-by":"publisher","first-page":"22","DOI":"10.3390\/tropicalmed4010022","article-title":"One health-its importance in helping to better control Antimicrobial Resistance. Trop med","volume":"4","author":"Collignon","year":"2019","journal-title":"Infect. Dis."},{"key":"ref24","doi-asserted-by":"publisher","first-page":"502","DOI":"10.1089\/fpd.2016.2258","article-title":"Presence of human pathogens in produce from retail Markets in Northern Germany","volume":"14","author":"Fiedler","year":"2017","journal-title":"Foodborne Pathog. Dis."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"2461","DOI":"10.1128\/AEM.02272-07","article-title":"Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes","volume":"74","author":"Frank","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"ref26","doi-asserted-by":"publisher","first-page":"1771","DOI":"10.1056\/NEJMoa1106483","article-title":"Epidemic profile of Shiga-toxin-producing Escherichia coli O104:H4 outbreak in Germany","volume":"365","author":"Frank","year":"2011","journal-title":"N. Engl. J. Med."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1016\/j.vetmic.2018.03.028","article-title":"Occurrence and characterisation of ESBL-encoding plasmids among Escherichia coli isolates from fresh vegetables","volume":"219","author":"Freitag","year":"2018","journal-title":"Vet. Microbiol."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"3011","DOI":"10.1017\/S0950268815000047","article-title":"Outbreaks attributed to fresh leafy vegetables, United States, 1973-2012","volume":"143","author":"Herman","year":"2015","journal-title":"Epidemiol. Infect."},{"key":"ref29","doi-asserted-by":"publisher","first-page":"2","DOI":"10.3389\/fmicb.2012.00002","article-title":"IncP-1epsilon plasmids are important vectors of antibiotic Resistance genes in agricultural systems: diversification driven by class 1 Integron gene cassettes","volume":"3","author":"Heuer","year":"2012","journal-title":"Front. Microbiol."},{"key":"ref30","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1111\/j.1574-6941.2002.tb01019.x","article-title":"Gentamicin resistance genes in environmental bacteria: prevalence and transfer","volume":"42","author":"Heuer","year":"2002","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"236","DOI":"10.1016\/j.mib.2011.04.009","article-title":"Antibiotic resistance gene spread due to manure application on agricultural fields","volume":"14","author":"Heuer","year":"2011","journal-title":"Curr. Opin. Microbiol."},{"key":"ref32","doi-asserted-by":"publisher","first-page":"e0203338","DOI":"10.1371\/journal.pone.0203338","article-title":"Extended-spectrum beta-lactamase producing Enterobacteriaceae (ESBL-E) isolated from bean sprouts in the Netherlands","volume":"13","author":"Huizinga","year":"2018","journal-title":"PLoS One"},{"key":"ref33","doi-asserted-by":"publisher","first-page":"1156","DOI":"10.1089\/mdr.2017.0198","article-title":"Extended-Spectrum beta-lactamase, AmpC, and MBL-producing gram-negative Bacteria on fresh vegetables and ready-to-eat salads sold in local markets","volume":"24","author":"Iseppi","year":"2018","journal-title":"Microb. Drug Resist."},{"key":"ref1002","doi-asserted-by":"publisher","first-page":"536","DOI":"10.1016\/j.tim.2014.05.005","article-title":"Fate and effects of veterinary antibiotics in soil","volume":"22","author":"Jechalke","year":"2014","journal-title":"Trends Microbiol."},{"key":"ref34","doi-asserted-by":"publisher","first-page":"1049","DOI":"10.1002\/jobm.202100201","article-title":"Antibiotic resistance genes in bacteria: occurrence, spread, and control","volume":"61","author":"Jian","year":"2021","journal-title":"J. Basic Microbiol."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1016\/j.vetmic.2019.03.025","article-title":"Diversity in prevalence and characteristics of ESBL\/pAmpC producing E. coli in food in Germany","volume":"233","author":"Kaesbohrer","year":"2019","journal-title":"Vet. Microbiol."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"103261","DOI":"10.1016\/j.fm.2019.103261","article-title":"Dynamics of bacterial communities in alfalfa and mung bean sprouts during refrigerated conditions","volume":"84","author":"Keshri","year":"2019","journal-title":"Food Microbiol."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"167671","DOI":"10.1016\/j.scitotenv.2023.167671","article-title":"Fresh produce as a reservoir of antimicrobial resistance genes: a case study of Switzerland","volume":"907","author":"Kl\u00e4ui","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1038\/s42003-019-0617-x","article-title":"DNA enrichment and tagmentation method for species-level identification and strain-level differentiation using ON-rep-seq","volume":"2","author":"Krych","year":"2019","journal-title":"Commun. Biol."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2013\/204141","article-title":"Modulation of bacterial multidrug Resistance efflux pumps of the major facilitator superfamily","volume":"2013","author":"Kumar","year":"2013","journal-title":"Int. J. Bacteriol."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"548","DOI":"10.1111\/j.1556-4029.2006.00131.x","article-title":"Environmental survey for four pathogenic bacteria and closely related species using phylogenetic and functional genes","volume":"51","author":"Kuske","year":"2006","journal-title":"J. Forensic Sci."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"e59310","DOI":"10.1371\/journal.pone.0059310","article-title":"Bacterial communities associated with the surfaces of fresh fruits and vegetables","volume":"8","author":"Leff","year":"2013","journal-title":"PLoS One"},{"key":"ref42","doi-asserted-by":"publisher","first-page":"110","DOI":"10.3390\/vetsci7030110","article-title":"Manure as a potential hotspot for antibiotic Resistance dissemination by horizontal gene transfer events","volume":"7","author":"Lima","year":"2020","journal-title":"Vet. Sci."},{"key":"ref43","doi-asserted-by":"publisher","first-page":"104188","DOI":"10.1016\/j.fm.2022.104188","article-title":"The conjugative transfer of plasmid-mediated mobile colistin resistance gene, mcr-1, to Escherichia coli O157:H7 and Escherichia coli O104:H4 in nutrient broth and in mung bean sprouts","volume":"111","author":"Luo","year":"2023","journal-title":"Food Microbiol."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1017\/S0950268808001969","article-title":"The growing burden of foodborne outbreaks due to contaminated fresh produce: risks and opportunities","volume":"137","author":"Lynch","year":"2009","journal-title":"Epidemiol. Infect."},{"key":"ref45","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/j.jgar.2021.07.019","article-title":"First report of mobile tigecycline resistance gene tet(X4)-harbouring multidrug-resistant Escherichia coli from wastewater in Norway","volume":"27","author":"Marathe","year":"2021","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref46","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1007\/s00003-015-1003-3","article-title":"Occurrence of Salmonella, L. monocytogenes, Shigatoxin-producing E. coli and ESBL-producing Enterobacteriaceae in sprout samples collected from the Swiss market","volume":"11","author":"Margot","year":"2016","journal-title":"J. Verbrauch. Lebensm."},{"key":"ref47","doi-asserted-by":"publisher","first-page":"2017","DOI":"10.1128\/microbiolspec.ARBA-0009-2017","article-title":"Antimicrobial Resistance: a one health perspective","volume":"6","author":"McEwen","year":"2018","journal-title":"Microbiol. Spectr."},{"key":"ref48","doi-asserted-by":"publisher","first-page":"352","DOI":"10.1099\/ijs.0.056994-0","article-title":"Taxonomic use of DNA G+C content and DNA\u2013DNA hybridization in the genomic age","volume":"64","author":"Meier-Kolthoff","year":"2014","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref49","doi-asserted-by":"publisher","first-page":"5536","DOI":"10.1128\/AEM.69.9.5536-5542.2003","article-title":"Plasmid transfer from Pseudomonas putida to the indigenous bacteria on alfalfa sprouts: characterization, direct quantification, and in situ location of transconjugant cells","volume":"69","author":"Molbak","year":"2003","journal-title":"Appl. Environ. Microbiol."},{"key":"ref50","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1016\/j.ijmm.2014.11.009","article-title":"Prevalence of subtilase cytotoxin-encoding subAB variants among Shiga toxin-producing Escherichia coli strains isolated from wild ruminants and sheep differs from that of cattle and pigs and is predominated by the new allelic variant subAB2-2","volume":"305","author":"Nuesch-Inderbinen","year":"2015","journal-title":"Int. J. Med. Microbiol."},{"key":"ref51","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.fm.2012.04.016","article-title":"Factors influencing the microbial safety of fresh produce: a review","volume":"32","author":"Olaimat","year":"2012","journal-title":"Food Microbiol."},{"key":"ref52","doi-asserted-by":"publisher","first-page":"723274","DOI":"10.3389\/fmolb.2021.723274","article-title":"Host - bacterial pathogen communication: the wily role of the multidrug efflux pumps of the MFS family","volume":"8","author":"Pasqua","year":"2021","journal-title":"Front. Mol. Biosci."},{"key":"ref53","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1016\/j.postharvbio.2007.01.001","article-title":"Role of microbiological and physiological spoilage mechanisms during storage of minimally processed vegetables","volume":"44","author":"Ragaert","year":"2007","journal-title":"Postharvest Biol. Technol."},{"key":"ref54","doi-asserted-by":"publisher","first-page":"1843","DOI":"10.1007\/s10096-014-2142-7","article-title":"Prevalence of ESBL-producing Enterobacteriaceae in raw vegetables","volume":"33","author":"Reuland","year":"2014","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref55","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1099\/mgen.0.000206","article-title":"MOB-suite: software tools for clustering, reconstruction and typing of plasmids from draft assemblies","volume":"4","author":"Robertson","year":"2018","journal-title":"Microb. Genom."},{"key":"ref56","doi-asserted-by":"publisher","first-page":"1600","DOI":"10.1093\/bioinformatics\/btaa900","article-title":"NanoCLUST: a species-level analysis of 16S rRNA nanopore sequencing data","volume":"37","author":"Rodriguez-Perez","year":"2021","journal-title":"Bioinformatics"},{"key":"ref57","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/j.ijfoodmicro.2011.06.001","article-title":"Antibiotic resistance in bacteria isolated from vegetables with regards to the marketing stage (farm vs. supermarket)","volume":"148","author":"Schwaiger","year":"2011","journal-title":"Int. J. Food Microbiol."},{"key":"ref58","doi-asserted-by":"publisher","first-page":"1461","DOI":"10.3201\/eid2508.180761","article-title":"Multistate outbreak of Listeriosis associated with packaged leafy green salads, United States and Canada, 2015-2016","volume":"25","author":"Self","year":"2019","journal-title":"Emerg. Infect. Dis."},{"key":"ref59","doi-asserted-by":"publisher","first-page":"2024","DOI":"10.4315\/0362-028X.JFP-15-556","article-title":"Multistate outbreak of Escherichia coli O157:H7 infections associated with consumption of fresh spinach: United States, 2006","volume":"79","author":"Sharapov","year":"2016","journal-title":"J. Food Prot."},{"key":"ref60","doi-asserted-by":"publisher","first-page":"102489","DOI":"10.1016\/j.plasmid.2020.102489","article-title":"The role of hosts, plasmids and environment in determining plasmid transfer rates: a meta-analysis","volume":"108","author":"Sheppard","year":"2020","journal-title":"Plasmid"},{"key":"ref61","doi-asserted-by":"publisher","first-page":"4854","DOI":"10.1128\/AEM.66.11.4854-4862.2000","article-title":"Exogenous isolation of antibiotic resistance plasmids from piggery manure slurries reveals a high prevalence and diversity of IncQ-like plasmids","volume":"66","author":"Smalla","year":"2000","journal-title":"Appl. Environ. Microbiol."},{"key":"ref62","doi-asserted-by":"publisher","first-page":"e0036124","DOI":"10.1128\/spectrum.00361-24","article-title":"Antibiotic resistance plasmids in Enterobacteriaceae isolated from fresh produce in northern Germany","volume":"12","author":"Stein","year":"2024","journal-title":"Microbiol. Spectr."},{"key":"ref63","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1093\/bioinformatics\/bti054","article-title":"Circular genome visualization and exploration using CGView","volume":"21","author":"Stothard","year":"2005","journal-title":"Bioinformatics"},{"key":"ref64","doi-asserted-by":"publisher","first-page":"626","DOI":"10.3201\/eid0505.990503","article-title":"Infections associated with eating seed sprouts: an international concern","volume":"5","author":"Taormina","year":"1999","journal-title":"Emerg. Infect. Dis."},{"key":"ref65","doi-asserted-by":"publisher","first-page":"6614","DOI":"10.1093\/nar\/gkw569","article-title":"NCBI prokaryotic genome annotation pipeline","volume":"44","author":"Tatusova","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref66","doi-asserted-by":"publisher","first-page":"1756","DOI":"10.1017\/S0950268815002769","article-title":"Are ready-to-eat salads ready to eat? An outbreak of Salmonella Coeln linked to imported, mixed, pre-washed and bagged salad, Norway, November 2013","volume":"144","author":"Vestrheim","year":"2016","journal-title":"Epidemiol. Infect."},{"key":"ref67","doi-asserted-by":"publisher","first-page":"173","DOI":"10.3389\/fmicb.2016.00173","article-title":"Dissemination of Antimicrobial Resistance in microbial ecosystems through horizontal gene transfer","volume":"7","author":"von Wintersdorff","year":"2016","journal-title":"Front. 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