{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:50:42Z","timestamp":1790787042840,"version":"4.1.0"},"reference-count":20,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T00:00:00Z","timestamp":1743033600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec><jats:title>Background and aims<\/jats:title><jats:p><jats:italic>Zingiber officinale<\/jats:italic> Rosc (Dry ginger) and <jats:italic>Schisandra chinensis<\/jats:italic> (Turcz.) Baill (Schisandra chinensis) drug pairs (DSP) are often used as drug pairs for the treatment of asthma, and these two traditional Chinese medicines (TCM) are also the core components of multiple TCM. However, its specific pharmacological mechanism needs further research. The aim of this study is to investigate the effects of DSP on intestinal flora and bile acid metabolism in rats with asthma caused by cold, and to provide experimental evidence for its clinical application.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Sixty male rats are divided into five groups, 12 rats per group. Except for control groups, other groups of rats use the method of \u201cabdominal injection of \u201cOVA + ice water swimming\u201d to establish cold cough rats models. After the administration cycle is over, an optical microscope count method is used to detect eosinophils (EOS) and neutrophils (Neu) in bronchoalveolar lavage fluid (BALF); Enzyme-Linked Immunosorbent Assay (ELISA) method detects Immunoglobulin E (IgE), Interleukin-13 (IL-13), Interleukin-4 (IL-4), Tumor Necrosis Factor-\u03b1 (TNF-\u03b1), Interferon-\u03b3 (INF-\u03b3); Western Blot (WB) and Reverse Transcription-Polymerase Chain Reaction (RT-PCR) analysis was used to detect Forkhead box protein P3 (Foxp3) and Transforming Growth Factor-beta 1 (TGF-\u03b21) proteins expression. In addition, Using 16S rDNA sequencing reveal the role of intestinal flora in asthma and the effect on the gut microbiome after DSP treatment. We also examined Farnesoid X Receptor (FXR) proteins expression, and finally used ultra performance UPLC-MS\/MS to analyze bile acids (BAs) contentin in rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>DSP inhibits asthma inflammation. It alleviates inflammatory factors, suppresses the inflammatory response in asthmatic rats by regulating FOXP3 and TGF-\u03b21 in Treg cells, and reduces tissue damage. After DSP treatment, intestinal flora changed: harmful bacteria like <jats:italic>Streptococcus<\/jats:italic> decreased, while beneficial bacteria such as <jats:italic>Candidatus - Arthromitus<\/jats:italic> and <jats:italic>Ligilactobacillus<\/jats:italic> increased. These changes can be potential markers for DSP-intervened asthma. Also, DSP increased FXR protein expression and changed the bile acid spectrum: Deoxycholic acid (DCA) increased, allocholic acid (ACA), glycolithocholic acid (GLCA), glycochenodeoxycholic acid (GCDCA) andglycoursodeoxycholic acid (GUDCA) decreased.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study has preliminarily revealed that DSP has the effect of alleviating inflammation levels, also regulate the expression of FOXP3 and TGF-\u03b21 proteins, and has an impact on the gut microbiota and bile acid metabolism, thereby exerting an improving effect on asthma and providing a reference for the clinical application of traditional Chinese medicine in the treatment of asthma.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2025.1541335","type":"journal-article","created":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T02:48:06Z","timestamp":1743130086000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Dry ginger and Schisandra chinensis modulate intestinal flora and bile acid metabolism to treatment asthma"],"prefix":"10.3389","volume":"16","author":[{"given":"Yantong","family":"Yu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Geng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Bu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanquan","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongmei","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Hong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,3,27]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"842","DOI":"10.1111\/cea.12253","article-title":"Low gut microbiota diversity in early infancy precedes asthma at school age","volume":"44","author":"Abrahamsson","year":"2014","journal-title":"Clin. 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Sci."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"85","DOI":"10.19577\/j.cnki.issn10074406.2001.02.008","article-title":"Comparison of the effects of taurine conjugation and its free bile acids on an anti-inflammatory model of cough suppression and expectoration in mice","volume":"2","author":"Hu","year":"2001","journal-title":"Chin. J. Clin. Pharm."},{"key":"ref6","first-page":"1","article-title":"Clinical observation and mechanism study of Pingkui Enema in the treatment of ulcerative colitis","volume":"8","author":"Jin","year":"2019","journal-title":"J. Nanjing Univ. Trad. Chin. Med."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"835","DOI":"10.14670\/hh-27.835","article-title":"Farnesoid X receptor (FXR) from normal to malignant state","volume":"27","author":"Koutsounas","year":"2012","journal-title":"Histol. Histopathol."},{"key":"ref8","first-page":"99-100+103","article-title":"Curative effect observation of Lingganwuweijiangxintang from synopsis of GoldenChamber treatment for cold fluid-mycoplasma pneumonia","volume":"51","author":"Lang","year":"2013","journal-title":"Chin. Mod. Doctor"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"8406","DOI":"10.3390\/ijms22168406","article-title":"An update on the role of Nrf2 in respiratory disease: molecular mechanisms and therapeutic approaches","volume":"22","author":"Lee","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"ref10","volume-title":"Experimental methodology of Chinese medicine pharmacology","author":"Li","year":"2006"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"2","DOI":"10.13193\/j.archtcm.2009.04.199.majm.032","article-title":"An analysis of the application characteristics of Gan Jiang and Wu Wei Zi in the treatment of cough by means of sutras","volume":"27","author":"Ma","year":"2009","journal-title":"Chin. Arch. Tradit. Chin. 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Pharmacol."},{"key":"ref15","first-page":"85","article-title":"Perbandingan efek pengikatan taurin dan asam empedu bebasnya pada model anti-inflamasi penekanan batuk dan ekspektasi pada tikus","volume":"2","author":"Si","year":"2002","journal-title":"Univ. Pertanian Mongolia Dalam"},{"key":"ref16","first-page":"8","article-title":"Mechanism of action of bile acid transporter proteins and metabolic enzymes in cholestasis","volume":"4","author":"Wu","year":"2018","journal-title":"J. Strait Pharmaceut."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"187","DOI":"10.13560\/j.cnki.biotech.bull.1985.2022-0931","article-title":"Towards the understanding on the physiological functions of bile acids and interactions with gut microbiota","volume":"39","author":"Xiong","year":"2023","journal-title":"Biotechnol. Bull."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"102843","DOI":"10.1016\/j.jbc.2022.102843","article-title":"TGF-\u03b21 suppresses the T-cell response in teleost fish by initiating Smad3- and Foxp3-mediated transcriptional networks","volume":"299","author":"Zhang","year":"2023","journal-title":"J. Biol. Chem."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"268","DOI":"10.13220\/j.cnki.jipr.2016.02.013","article-title":"Research progress in pharmacological effects and quantitative methods for biles acids in Calculus bovis","volume":"43","author":"Zhang","year":"2016","journal-title":"J. Int. Pharmaceut. Res."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"117614","DOI":"10.1016\/j.jep.2023.117614","article-title":"A comprehensive study of Ephedra sinica Stapf-Schisandra chinensis (Turcz.) Baill herb pair on airway protection in asthma","volume":"322","author":"Zhuo","year":"2024","journal-title":"J. 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