{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T16:06:36Z","timestamp":1753891596450,"version":"3.41.2"},"reference-count":29,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T00:00:00Z","timestamp":1742169600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LTGY23H190003"],"award-info":[{"award-number":["LTGY23H190003"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>This study isolated a <jats:italic>Klebsiella pasteurii<\/jats:italic> strain, K1134, from the sputum of an ICU patient, revealing its resistance to the carbapenem antibiotics meropenem and imipenem. Whole-genome sequencing identified a plasmid pK1134-KPC, which carries the carbapenem resistance gene <jats:italic>bla<\/jats:italic><jats:sub>KPC-2<\/jats:sub>. pK1134-KPC, belonging to the IncFII<jats:sub>pCP020359<\/jats:sub> plasmid group, exhibits a modular structure with <jats:italic>bla<\/jats:italic><jats:sub>KPC-2<\/jats:sub> embedded in a 32.09\u202fkb accessory region containing multiple accessory genetic elements (AGEs). Comparative genomic analysis of 48 <jats:italic>K. pasteurii<\/jats:italic> isolates from 12 countries showed high genetic diversity, with strains clustered into three clades. Notably, <jats:italic>K. pasteurii<\/jats:italic> harbors extensive antimicrobial resistance genes across diverse AGEs, classifying it as multidrug-resistant. Twelve <jats:italic>bla<\/jats:italic><jats:sub>KPC<\/jats:sub>-carrying AGEs were identified from the sequences of the isolates, classified into two groups: Tn<jats:italic>7551<\/jats:italic> and Tn<jats:italic>6296<\/jats:italic>-related elements. The gene clusters for enterotoxins tilimycin and tilivalline, encompassing key regulators and operons, were present in nearly all strains, with incomplete clusters exclusively observed in clade 3 isolates. This study underscores the global dissemination and genetic adaptability of <jats:italic>K. pasteurii<\/jats:italic>, highlighting its potential role as a reservoir for resistance genes and emphasizing the need for robust surveillance to mitigate its public health impact.<\/jats:p>","DOI":"10.3389\/fmicb.2025.1561624","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T07:22:18Z","timestamp":1742196138000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Unraveling the genomic epidemiology and plasmid-mediated carbapenem resistance of Klebsiella pasteurii"],"prefix":"10.3389","volume":"16","author":[{"given":"Xinyue","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zexuan","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinshuo","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingguang","family":"Jin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanxia","family":"Wan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huimin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinhua","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,3,17]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1449","DOI":"10.1007\/s10096-023-04682-x","article-title":"Country-wide expansion of a VIM-1 carbapenemase-producing Klebsiella oxytoca ST145 lineage in Poland, 2009\u20132019","volume":"42","author":"Biedrzycka","year":"2023","journal-title":"Eur. 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