{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T17:29:27Z","timestamp":1773422967693,"version":"3.50.1"},"reference-count":42,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T00:00:00Z","timestamp":1741219200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p><jats:italic>Chlamydia psittaci<\/jats:italic> is a zoonotic pathogen that causes an acute disease known as psittacosis. To establish infection in host cells, <jats:italic>Chlamydia<\/jats:italic> manipulates the host cell\u2019s membrane trafficking pathways.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, using fluorescently labeled <jats:italic>C. psittaci<\/jats:italic> and screening a human membrane trafficking small interfering RNA (siRNA) library, we identified 34 host proteins that influenced <jats:italic>C. psittaci<\/jats:italic> infection in HeLa cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among these, knockdown (KD) of two genes encoding subunits of the coatomer complex I (COPI) inhibited the pathogen\u2019s intracellular survival. Specifically, the knockdown of COPB1, a COPI subunit, significantly reduced the intracellular proliferation of <jats:italic>C. psittaci<\/jats:italic>. Mechanistically, we found that type I interferon negatively affected <jats:italic>C. psittaci<\/jats:italic> infection. Moreover, COPB1 KD disrupted the homeostasis of STING, preventing its retrieval from the Golgi back to the endoplasmic reticulum (ER), which in turn activated type I interferon signaling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Together, our findings advance the understanding of the mechanisms underlying <jats:italic>Chlamydia<\/jats:italic> infection and offer potential avenues for the development of new anti-<jats:italic>C. psittaci<\/jats:italic> strategies.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2025.1566239","type":"journal-article","created":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T06:51:24Z","timestamp":1741243884000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["COPB1-knockdown induced type I interferon signaling activation inhibits Chlamydia psittaci intracellular proliferation"],"prefix":"10.3389","volume":"16","author":[{"given":"Nana","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huiying","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yufei","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinhui","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoxiao","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuchen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yonghui","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuan","family":"Ouyang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yujun","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yajun","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Jiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,3,6]]},"reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"e12857","DOI":"10.1111\/cmi.12857","article-title":"The recycling endosome and bacterial pathogens.","volume":"20","author":"Allgood","year":"2018","journal-title":"Cell Microbiol."},{"key":"B2","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1242\/jcs.02733","article-title":"Trafficking from CD63-positive late endocytic multivesicular bodies is essential for intracellular development of Chlamydia trachomatis.","volume":"119","author":"Beatty","year":"2006","journal-title":"J. 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