{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:56:02Z","timestamp":1787842562616,"version":"build-2784847793"},"reference-count":28,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T00:00:00Z","timestamp":1748995200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32160544"],"award-info":[{"award-number":["32160544"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31801523"],"award-info":[{"award-number":["31801523"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p><jats:italic>Schizosaccharomyces japonicus<\/jats:italic> belongs to fermentative non-<jats:italic>Saccharomyces<\/jats:italic> yeast, which has been reported to be predominant in the late stage of natural wine fermentation, and can coexist with <jats:italic>S. cerevisiae<\/jats:italic>. This study targeted the indigenous <jats:italic>S. japonicus<\/jats:italic> strains found in Guizhou, China, to explore their genotype diversity, and to further analyze their fermentation properties especially on the degradation capacity of malic acid. Seven microsatellite loci were developed including GA1, CG3, SyGAA2, C11, SaGAA1, C12, and TG2, and 43 genotypes were identified from 63 <jats:italic>S. japonicus<\/jats:italic> strains. All <jats:italic>S. japonicus<\/jats:italic> strains showed high malic acid reduction rate (higher than 90%) by initial metabolic analysis, except FBKL2.9SZJ-43 showing medium malic acid reduction rate (89.47%). The highest malic acid reduction rate was found in FBKL2.9SZJ-37 (98.64%). Ten <jats:italic>S. japonicus<\/jats:italic> strains were selected for further simulated fermentations based on their genotype, flocculation, and organic acid degradation traits. Among them, FBKL2.9SZJ-37 and FBKL2.9SZJ-55 showed good fermentation ability, strong malic acid degradation capacity, and relative weak tartaric acid degradation performance under both simulated fermentation conditions. Further fermentations using <jats:italic>Rosa sterilis<\/jats:italic>, <jats:italic>Rosa roxbunghii<\/jats:italic> Tratt <jats:italic>juice<\/jats:italic>, and <jats:italic>Cabernet Sauvignon<\/jats:italic>, verified the fermentation characteristics of the two <jats:italic>S. japonicus<\/jats:italic> strains when compared to <jats:italic>S. cerevisiae<\/jats:italic>. Especially for FBKL2.9SZJ-37, it showed good ethanol production, stronger glycerol formation and acid degradation ability, with malic acid reduction rate being 60.50, 64.98 and 98.32% in fermentations of three fruits, respectively. This study displays initial explorations on genotyping and fermentation application of indigenous <jats:italic>S. japonicus<\/jats:italic> especially for malic acid type fruit wine making, which would help to the development and application of potential excellent non-<jats:italic>Saccharomyces<\/jats:italic> yeast sources.<\/jats:p>","DOI":"10.3389\/fmicb.2025.1569585","type":"journal-article","created":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T05:39:08Z","timestamp":1749015548000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Targeting indigenous Schizosaccharomyces japonicus for genotype exploration and organic acid degradation analysis"],"prefix":"10.3389","volume":"16","author":[{"given":"Qiling","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunxiao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhanyan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhangyu","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenya","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaojiao","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angxin","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuangen","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuyi","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lizhen","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,6,4]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"2094","DOI":"10.3846\/j.issn.0578-175.2011.10.014","article-title":"Analysis of organic acid components and ascorbate content in roxburgh rose","volume":"44","author":"An","year":"2011","journal-title":"Agric. 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Res."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"127","DOI":"10.5073\/vitis.2019.58.special-issue.127-133","article-title":"Modification of the organic acid profile of grapes due to climate changes alters the stability of red wine pH phenolics during controlled oxidation","volume":"58","author":"Picariello","year":"2019","journal-title":"Vitis"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"113009","DOI":"10.1016\/j.lwt.2021.113009","article-title":"Schizosaccharomyces japonicus\/Saccharomyces cerevisiae mixed starter cultures: new perspectives for the improvement of Sangiovese aroma, taste, and color stability","volume":"156","author":"Portaro","year":"2022","journal-title":"LWT"},{"key":"ref16","doi-asserted-by":"publisher","first-page":"14","DOI":"10.3390\/fermentation4010014","article-title":"Schizosaccharomyces japonicus: a polysaccharide-overproducing yeast to be used in winemaking","volume":"4","author":"Romani","year":"2018","journal-title":"Fermentation"},{"key":"ref17","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1007\/s10482-006-9104-8","article-title":"The genetic structure of fermentative vineyard-associated Saccharomyces cerevisiae populations revealed by microsatellite analysis","volume":"91","author":"Schuller","year":"2007","journal-title":"Antonie Van Leeuwenhoek"},{"key":"ref18","article-title":"Healthy people and a healthy planet","volume-title":"Nature","author":"Shan","year":"2024"},{"key":"ref19","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1007\/s00294-003-0411-6","article-title":"Malo-ethanolic fermentation in Saccharomyces and Schizosaccharomyces","volume":"43","author":"Volschenk","year":"2003","journal-title":"Curr. Genet."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"111580","DOI":"10.1016\/j.foodres.2022.111580","article-title":"The impact of indigenous Saccharomyces cerevisiae and Schizosaccharomyces japonicus on typicality of crystal grape (Niagara) wine","volume":"159","author":"Wang","year":"2022","journal-title":"Food Res. Int."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"108582","DOI":"10.1016\/j.foodres.2019.108582","article-title":"Yeast diversity investigation of Vitis davidii F\u00f6ex during spontaneous fermentations using culture-dependent and high-throughput sequencing approaches","volume":"126","author":"Wang","year":"2019","journal-title":"Food Res. Int."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"529","DOI":"10.3864\/j.issn.0578-1752.2023.03.011","article-title":"Progress in wine fermentation applications of non-Saccharomyces","volume":"56","author":"Wang","year":"2023","journal-title":"Agric. Sci. China"},{"key":"ref23","article-title":"A Schizosaccharomyces japonicus strain with high flocculation trait and its application","author":"Wang","year":"2024"},{"key":"ref24","doi-asserted-by":"publisher","first-page":"279","DOI":"10.7506\/spkx1002-6630-201021063","article-title":"Screening and identification of degradable L-malic acid and citric acid strains","volume":"31","author":"Wang","year":"2011","journal-title":"Food Sci."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1016\/0888-7543(90)90195-Z","article-title":"Informativeness of human (dC\u202f\u2212\u202fdA)n\u00b7(dG\u202f\u2212\u202fdT)n polymorphisms","volume":"7","author":"Weber","year":"1990","journal-title":"J. Genet. Genomics"},{"key":"ref26","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1002\/j.2050-0416.1976.tb03731.x","article-title":"Organic acid metabolism of yeasts during fermentation of alcoholic beverages\u2014a review","volume":"82","author":"Whiting","year":"1976","journal-title":"J. Inst. Brew."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"244","DOI":"10.13684\/j.cnki.spkj.2016.11.052","article-title":"Analysis of organic acid content in wine grapes at the eastern foot of Helan Mountain","volume":"41","author":"Yang","year":"2016","journal-title":"Food Technol."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"3431","DOI":"10.13343\/j.cnki.wsxb.20210004","article-title":"Analysis of genotype diversity of wild S. cerevisiae in natural fermentation of Chinese thorn grape in Ziyun, Guizhou","volume":"61","author":"Yang","year":"2021","journal-title":"J. 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