{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T18:17:59Z","timestamp":1790965079830,"version":"4.1.0"},"reference-count":59,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T00:00:00Z","timestamp":1749513600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>The global dissemination of extended-spectrum \u03b2-lactamase (ESBL)-producing <jats:italic>Escherichia coli<\/jats:italic> (<jats:italic>E. coli<\/jats:italic>) poses a significant public health challenge, particularly in regions with high antimicrobial resistance (AMR) occurrence. This study investigated the occurrence, genomic characteristics, and dissemination dynamics of ESBL-producing <jats:italic>E. coli<\/jats:italic> in Abruzzo, Italy, by analyzing 956 isolates from humans, livestock, wildlife, and food products.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Phenotypic and genomic analyses were performed on the isolates to assess ESBL-<jats:italic>E. coli<\/jats:italic> occurrence and characteristics. Multilocus sequence typing (MLST) was used to identify sequence types (STs), and plasmid profiling alongside synteny analysis was conducted to investigate horizontal gene transfer and resistance gene integration. Spatial analysis was also carried out to identify hotspots of ESBL-positive isolates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>An overall ESBL-<jats:italic>E. coli<\/jats:italic> occurrence of 14.1% (135\/956 samples) was found, with significant variation across hosts: companion animals exhibited the highest occurrence (16.2%), followed by livestock and food matrices (14.6%), and wildlife (7.0%). Spatial analysis identified a hotspot in northeastern Abruzzo, where ESBL-positive isolates were 5.34 times more likely to occur (<jats:italic>p<\/jats:italic>\u202f&amp;lt;\u202f0.001). MLST identified 58 sequence types (STs), with ST131 dominating human isolates (12\/19). In cattle, predominant sequence types were ST16565 (5 isolates) and ST540 (4 isolates); in poultry, ST43 (5 isolates), ST10 (4 isolates), and ST6215 (3 isolates) were most common; ST206 (8 isolates) was predominant in swine; and in dogs, ST10 (4 isolates) and ST3580 (3 isolates) were most prevalent. Genomic analysis revealed host-specific distributions of ESBL genes: <jats:italic>bla<\/jats:italic><jats:sub>CTX-M-15<\/jats:sub> predominated in humans and dogs, while <jats:italic>bla<\/jats:italic><jats:sub>CTX-M-1<\/jats:sub> was most common in pigs. Plasmid profiling revealed IncF and IncI plasmids as key vectors for horizontal gene transfer. Synteny analysis showed identical flanking regions of <jats:italic>bla<\/jats:italic><jats:sub>CTX-M-1<\/jats:sub> and <jats:italic>bla<\/jats:italic><jats:sub>CTX-M-15<\/jats:sub> across phylogenetically distant strains, suggesting chromosomal integration and stable maintenance of resistance genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>These findings underscore the interconnectedness of human, animal, and environmental reservoirs in AMR dissemination. The high genetic diversity observed within farms and the detection of shared clusters across hosts emphasize the need for integrated One Health interventions, including reduced antibiotic use in livestock and enhanced surveillance of high-risk environments. This study provides critical insights into local AMR dynamics, offering a model for regional mitigation strategies.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2025.1595652","type":"journal-article","created":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T05:22:50Z","timestamp":1749532970000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":15,"title":["Comprehensive regional study of ESBL Escherichia coli: genomic insights into antimicrobial resistance and inter-source dissemination of ESBL genes"],"prefix":"10.3389","volume":"16","author":[{"given":"Lisa","family":"Di Marcantonio","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofia","family":"Chiatamone Ranieri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michela","family":"Toro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice","family":"Marchegiano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Cito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia","family":"Sulli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilaria","family":"Del Matto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria","family":"Di Lollo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Alessiani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Foschi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilenia","family":"Platone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimiliano","family":"Paoletti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"D\u2019Alterio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuliano","family":"Garofolo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Janowicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,6,10]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"fiac045","DOI":"10.1093\/femsec\/fiac045","article-title":"Hiding in plain sight-wildlife as a neglected reservoir and pathway for the spread of antimicrobial resistance: a narrative review","volume":"98","author":"Abbassi","year":"2022","journal-title":"FEMS Microbiol. 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Microbiol."},{"key":"ref29","doi-asserted-by":"publisher","first-page":"675","DOI":"10.5455\/javar.2024.k817","article-title":"A review of antimicrobial usage practice in livestock and poultry production and its consequences on human and animal health","volume":"11","author":"Islam","year":"2024","journal-title":"J. Adv. Vet. Anim. Res."},{"key":"ref30","doi-asserted-by":"publisher","first-page":"888","DOI":"10.1111\/zph.12985","article-title":"Occurrence and potential transmission of extended-spectrum beta-lactamase-producing extraintestinal pathogenic and enteropathogenic Escherichia coli in domestic dog faeces from Minnesota","volume":"69","author":"Johnson","year":"2022","journal-title":"Zoonoses Public Health"},{"key":"ref31","doi-asserted-by":"publisher","first-page":"232","DOI":"10.14202\/IJOH.2021.232-236","article-title":"Prevalence of extended-spectrum \u03b2-lactamase-producing Escherichia coli in companion dogs in animal clinics, Surabaya, Indonesia","volume":"7","author":"Kristianingtyas","year":"2021","journal-title":"Int. J. One Health"},{"key":"ref32","doi-asserted-by":"publisher","first-page":"29459","DOI":"10.1038\/s41598-024-80943-9","article-title":"Characteristics of the extended-spectrum-\u03b2-lactamase-producing Escherichia coli isolated from diseased livestock and poultry in Taiwan","volume":"14","author":"Kuan","year":"2024","journal-title":"Sci. Rep."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"1134","DOI":"10.3390\/antibiotics13121134","article-title":"Diversity and Resistance Profiles of ESBL-Producing Gram-Negative Bacteria from Dairy Farms in Southern T\u00fcrkiye","volume":"13","author":"K\u00fcrekci","year":"2024","journal-title":"Antibiotics"},{"key":"ref34","doi-asserted-by":"publisher","first-page":"873989","DOI":"10.3389\/fcimb.2022.873989","article-title":"Wildlife and Antibiotic Resistance","volume":"12","author":"Laborda","year":"2022","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1038\/s41579-021-00649-x","article-title":"Antibiotic Resistance in the Environment","volume":"20","author":"Larsson","year":"2022","journal-title":"Nat. Rev. Microbiol."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"1219008","DOI":"10.3389\/fmicb.2023.1219008","article-title":"Occurrence and temporal distribution of extended-spectrum \u03b2-lactamase-producing Escherichia coli in clams from the Central Adriatic, Italy","volume":"14","author":"Leoni","year":"2023","journal-title":"Front. Microbiol."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"W256","DOI":"10.1093\/nar\/gkz239","article-title":"Interactive Tree Of Life (iTOL) v4: Recent Updates and New Developments","volume":"47","author":"Letunic","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"1394814","DOI":"10.3389\/fvets.2024.1394814","article-title":"Antibiotic-resistant characteristics and horizontal gene transfer ability analysis of extended-spectrum \u03b2-lactamase-producing Escherichia coli isolated from giant pandas","volume":"11","author":"Liu","year":"2024","journal-title":"Front. Vet. Sci."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"100910","DOI":"10.1016\/j.onehlt.2024.100910","article-title":"Antimicrobial resistance in Escherichia coli and Staphylococcus aureus at human-animal interfaces on Chongming Island, Shanghai: A One Health perspective","volume":"19","author":"Lv","year":"2024","journal-title":"One Health"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1145\/3125571.3125585","article-title":"RAWGraphs: A Visualisation Platform to Create Open Outputs","volume-title":"Proceedings of the 12th Biannual Conference on Italian SIGCHI Chapter","author":"Mauri","year":"2017"},{"key":"ref41","doi-asserted-by":"publisher","first-page":"e0179123","DOI":"10.1128\/aem.01791-23","article-title":"Genomic epidemiology of third-generation cephalosporin-resistant Escherichia coli from Argentinian pig and dairy farms reveals animal-specific patterns of co-resistance and resistance mechanisms","volume":"90","author":"Mounsey","year":"2024","journal-title":"Appl. Environ. Microbiol."},{"key":"ref42","doi-asserted-by":"publisher","DOI":"10.12834\/VetIt.2984.22731.2","article-title":"Antibiotic resistance, virulence genes, and phylogenetic groups of bacteria isolated from wild passerine birds in Iran","volume":"60","author":"Mousavinezhad","year":"2024","journal-title":"Vet. Ital."},{"key":"ref43","article-title":"Stemming the superbug tide: Just a few dollars more","volume-title":"OECD Publishing","year":"2018"},{"key":"ref44","doi-asserted-by":"publisher","first-page":"2091","DOI":"10.3389\/fmicb.2019.02091","article-title":"A State-of-Art Review on Multi-Drug Resistant Pathogens in Foods of Animal Origin: Risk Factors and Mitigation Strategies","volume":"10","author":"P\u00e9rez-Rodr\u00edguez","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref45","doi-asserted-by":"publisher","first-page":"1208","DOI":"10.3390\/antibiotics13121208","article-title":"Dairy Cattle and the Iconic Autochthonous Cattle in Northern Portugal Are Reservoirs of Multidrug-Resistant Escherichia coli","volume":"13","author":"Quinteira","year":"2024","journal-title":"Antibiotics (Basel)"},{"key":"ref46","doi-asserted-by":"publisher","first-page":"1249","DOI":"10.3390\/antibiotics12081249","article-title":"Fecal Carriage of Extended-Spectrum \u03b2-Lactamase-\/AmpC-Producing Escherichia coli in Pet and Stray Cats","volume":"12","author":"Ratti","year":"2023","journal-title":"Antibiotics (Basel)"},{"key":"ref47","doi-asserted-by":"publisher","first-page":"e000206","DOI":"10.1099\/mgen.0.000206","article-title":"MOB-Suite: Software Tools for Clustering, Reconstruction and Typing of Plasmids from Draft Assemblies","volume":"4","author":"Robertson","year":"2018","journal-title":"Microb. Genom."},{"key":"ref48","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.vetmic.2014.01.017","article-title":"Extended-spectrum \u03b2-lactamase, carbapenemase and AmpC producing Enterobacteriaceae in companion animals","volume":"170","author":"Rubin","year":"2014","journal-title":"Vet. Microbiol."},{"key":"ref49","doi-asserted-by":"publisher","first-page":"171","DOI":"10.3390\/antibiotics10020171","article-title":"Transmission Chains of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae at the Companion Animal Veterinary Clinic-Household Interface","volume":"10","author":"Schmitt","year":"2021","journal-title":"Antibiotics (Basel)"},{"key":"ref50","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1016\/j.drup.2012.12.001","article-title":"Extended-Spectrum Cephalosporin-Resistant Gram-Negative Organisms in Livestock: An Emerging Problem for Human Health?","volume":"16","author":"Seiffert","year":"2013","journal-title":"Drug Resist. Updates"},{"key":"ref51","doi-asserted-by":"publisher","first-page":"120814","DOI":"10.1016\/j.envres.2025.120814","article-title":"Investigating the antibiotic resistance genes and mobile genetic elements in water systems impacted with anthropogenic pollutants","volume":"269","author":"Sharma","year":"2025","journal-title":"Environ. Res."},{"key":"ref52","doi-asserted-by":"publisher","first-page":"e02227","DOI":"10.1128\/mBio.02227-15","article-title":"Antimicrobial Resistance in Agriculture","volume":"7","author":"Thanner","year":"2016","journal-title":"MBio"},{"key":"ref53","doi-asserted-by":"publisher","first-page":"e0241323","DOI":"10.1128\/spectrum.02413-23","article-title":"Flankophile: A Bioinformatic Pipeline for Prokaryotic Genomic Synteny Analysis","volume":"12","author":"Thorn","year":"2024","journal-title":"Microbiol. Spectr."},{"key":"ref54","doi-asserted-by":"publisher","first-page":"1350","DOI":"10.1126\/science.aao1495","article-title":"Reducing antimicrobial use in food animals","volume":"357","author":"Van Boeckel","year":"2017","journal-title":"Science"},{"key":"ref55","doi-asserted-by":"publisher","DOI":"10.12834\/VetIt.2569.18469.2","article-title":"First molecular detection of shiga toxin-producing Escherichia coli in dogs from serbia: a potential threat to human health?","volume":"59","author":"Vra\u010dar","year":"2023","journal-title":"Vet. Ital."},{"key":"ref56","doi-asserted-by":"publisher","first-page":"365","DOI":"10.2147\/IDR.S427571","article-title":"Whole-Genome Sequencing of an Escherichia coli ST69 Strain Harboring blaCTX-M-27 on a Hybrid Plasmid","volume":"17","author":"Wang","year":"2024","journal-title":"Infect Drug Resist."},{"key":"ref57","doi-asserted-by":"publisher","first-page":"105097","DOI":"10.1016\/j.ebiom.2024.105097","article-title":"Global transmission of extended-spectrum cephalosporin resistance in Escherichia coli driven by epidemic plasmids","volume":"103","author":"Zamudio","year":"2024","journal-title":"EBioMedicine"},{"key":"ref58","doi-asserted-by":"publisher","first-page":"2640","DOI":"10.1093\/jac\/dks261","article-title":"Identification of Acquired Antimicrobial Resistance Genes","volume":"67","author":"Zankari","year":"2012","journal-title":"J. Antimicrob. 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