{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T16:07:36Z","timestamp":1753891656387,"version":"3.41.2"},"reference-count":44,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T00:00:00Z","timestamp":1749168000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>In recent years, the escalating prevalence of Vancomycin-resistant <jats:italic>Enterococcus faecium<\/jats:italic> (VREfm) has emerged as a formidable challenge to global healthcare systems. While Matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has become an indispensable tool for bacterial identification, its potential for rapid discrimination between Vancomycin-susceptible <jats:italic>Enterococcus faecium<\/jats:italic> (VSEfm) and VREfm through characteristic peak analysis remains an area of active investigation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, we conducted literature search through databases to summarize the distribution of regionally prevalent VSEfm\/ VREfm characteristic peaks, and further collected mass spectrometry data from Meizhou People\u2019s Hospital (Guangdong, China) from 2021 to 2024 to explore stable characteristic peaks for both VSEfm and VREfm.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Through MALDI-TOF MS analysis, we identified stable characteristic peaks for both VSEfm (m\/z 3299.95\u202f\u00b1\u202f3.99 and m\/z 6605.13\u202f\u00b1\u202f7.28) and VREfm (m\/z 3313.01\u202f\u00b1\u202f2.76 and m\/z 6631.03\u202f\u00b1\u202f4.38) in the Meizhou region, with their discriminative efficacy validated by ROC curve analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>Our findings not only demonstrate the regional specificity of these characteristic peaks but also establish a robust methodological framework for rapid VSEfm\/VREfm differentiation. This advancement holds significant promise for guiding clinical decision-making and controlling VREfm dissemination. Nevertheless, we acknowledge the necessity for ongoing technological refinement to enhance the accuracy and broader applicability of this approach in diverse clinical settings.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2025.1603986","type":"journal-article","created":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T05:23:07Z","timestamp":1749187387000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Application of matrix-assisted laser desorption ionization time-of-flight mass spectrometry in the detection of vancomycin-resistant and-susceptible Enterococcus faecium"],"prefix":"10.3389","volume":"16","author":[{"given":"Wei","family":"He","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xintong","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueqin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liangming","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuemin","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,6,6]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"106911","DOI":"10.1016\/j.mimet.2024.106911","article-title":"Factors affecting the quality and reproducibility of MALDI-TOF MS identification for human Capnocytophaga species","volume":"220","author":"Algahawi","year":"2024","journal-title":"J. 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