{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T02:10:06Z","timestamp":1778119806027,"version":"3.51.4"},"reference-count":15,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T00:00:00Z","timestamp":1751587200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Multidrug-resistant organisms (MDROs) have become a serious public health concern worldwide. Screening for MDROs can encompass various phenotypic and genotypic approaches. Recently, culture-based MDROs screening has substantially benefitted from laboratory automation and artificial intelligence.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To evaluate the performance of PhenoMATRIX\u00ae PLUS for an institution-wide culture-based rectal screening for CPOs, ESBL-E, <jats:italic>Acinetobacter<\/jats:italic>, and VRE carriage.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The number of non-duplicated rectal Eswabs specimens used to generate culture plate images for the PhenoMATRIX\u00ae PLUS machine learning phase amounted to 2\u2032900 for chromID\u2122 ESBL agar, 2\u2032403 for chromID\u2122 OXA-48 agar, 2\u2032031 for CHROMagar\u2122 <jats:italic>Acinetobacter<\/jats:italic> agar, and 9\u2032333 for chromID\u2122 VRE agar. All such plates and images were classified by manual reading as either positive or negative. The validation of the settings derived during the learning phase was achieved on an additional 4\u2032517 non-duplicate specimens. Digital images of 22\u2032585 media plates (i.e., five per specimen) were prospectively analyzed by PhenoMATRIX\u00ae PLUS. All media plates were incubated in the WASPLab\u00ae and imaged at predefined time points.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>According to the manual work-up results, the output agreements of the PhenoMATRIX\u00ae PLUS for the three results \u201c<jats:italic>no growth<\/jats:italic>,\u201d \u201c<jats:italic>negative<\/jats:italic>,\u201d and \u201c<jats:italic>Send to Reader<\/jats:italic>\u201d reached 98.5, 99.6%. and 74.8%, respectively. Importantly, no false negative results were returned by PhenoMATRIX\u00ae PLUS in the present study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>PhenoMATRIX\u00ae PLUS contributes to a significant reduction in the workload. Therefore, technologists\u2019 routine tasks have evolved to the confirmatory tests of the presumptively positive colonies, allowing better management of the laboratory staff and the resources.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2025.1624411","type":"journal-article","created":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T05:28:27Z","timestamp":1751606907000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Evaluation of PhenoMATRIX\u00ae PLUS for the culture-based rectal screening of CPOs, ESBL-E, Acinetobacter, and VRE carriage"],"prefix":"10.3389","volume":"16","author":[{"given":"Abdessalam","family":"Cherkaoui","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gesuele","family":"Renzi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mireille","family":"Tittel-Elmer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacques","family":"Schrenzel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,7,4]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"775","DOI":"10.3390\/microorganisms13040775","article-title":"Experience in implementing colonization screening in a Multidisciplinary County clinical Hospital in Romania","volume":"13","author":"Birlutiu","year":"2025","journal-title":"Microorganisms"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"1063","DOI":"10.1007\/s10096-020-03817-8","article-title":"Rapid identification by MALDI-TOF\/MS and antimicrobial disk diffusion susceptibility testing for positive blood cultures after a short incubation on the WASPLab","volume":"39","author":"Cherkaoui","year":"2020","journal-title":"Eur. 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