{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T07:33:04Z","timestamp":1781335984759,"version":"3.54.1"},"reference-count":30,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,8,14]],"date-time":"2025-08-14T00:00:00Z","timestamp":1755129600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Feline infectious peritonitis (FIP), caused by feline coronavirus (FCoV), is a highly lethal disease characterized by systemic organ infection in cats. Current challenges of FIP include the absence of definitive diagnostic criteria, effective vaccines, and targeted therapies. Developing a robust genome editing toolkit is therefore critical to unraveling FCoV replication and pathogenesis mechanisms, elucidating viral protein functions, and identifying promising diagnostic and therapeutic targets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, we developed a yeast-based CRISPR genome editing system compatible with a TAR-generated FCoV infectious clone, enabling gene deletion, gene insertion, and point mutation with high efficiencies and accuracies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results and discussion<\/jats:title><jats:p>This system not only will contribute to a better understanding of the pathogenic mechanisms of FCoV but also serves as a valuable platform for vaccine development. Furthermore, it offers a possible strategy for genome editing and reverse genetics for other coronaviruses.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2025.1627509","type":"journal-article","created":{"date-parts":[[2025,8,14]],"date-time":"2025-08-14T05:34:56Z","timestamp":1755149696000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Development of a yeast-based CRISPR genome editing system for feline coronavirus"],"prefix":"10.3389","volume":"16","author":[{"given":"Xiaohu","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingru","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yueping","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,8,14]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"e00650","DOI":"10.1128\/mBio.00650-13","article-title":"Engineering a replication-competent, propagation-defective Middle East respiratory syndrome coronavirus as a vaccine candidate","volume":"4","author":"Almaz\u00e1n","year":"2013","journal-title":"MBio"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"5516","DOI":"10.1073\/pnas.97.10.5516","article-title":"Engineering the largest RNA virus genome as an infectious bacterial artificial chromosome","volume":"97","author":"Almaz\u00e1n","year":"2000","journal-title":"Proc. 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