{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T16:40:25Z","timestamp":1770914425353,"version":"3.50.1"},"reference-count":129,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T00:00:00Z","timestamp":1770854400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Open University and the Research England Expanding Excellence in England","award":["124.18"],"award-info":[{"award-number":["124.18"]}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Under its thick ice layer, Europa contains a shielded liquid water ocean where habitable conditions may exist. To effectively assess the habitability of this environment and the implications on putative biosignature formation, it is essential to integrate our understanding of the physicochemical conditions of the sub-surface ocean with ground-truth analysis on Earth, using both natural analogue sites and laboratory simulation experiments. This combined approach is particularly prudent for Europa, as locations proposed as natural analogues for the chemistry of Europa\u2019s ocean are predominantly located at ambient pressure (~0.1 MPa), which differs even from the shallowest depths of Europa\u2019s ocean (e.g., 20 to 30 MPa). Basque Lake No. 2, British Columbia, Canada, was used as geochemical analogue for the ice shell-ocean interface and sub-ice environment of Europa due to the Mg-Na-SO\n                    <jats:sub>4<\/jats:sub>\n                    chemistry (maximum 30 to 40% salinity in the summer) and temperature extremes [can reach \u221245 \u00b0C at night in the winter. In this study, microorganisms from the site were grown at elevated pressures in fluid medium based on a model of Europa\u2019s ocean chemistry, mimicking the conditions at Europa\u2019s upper ocean. Following incubation at successively higher pressures, (0.2, 10, 20 and 30 MPa) a microorganism with 99.1% 16S rRNA gene sequence homology to Pseudodesulfovibrio aespoeensis was isolated at 30 MPa (designated Pseudodesulfovibrio sp. OU_01). To our knowledge, this is the first study to demonstrate that microorganisms from an analogue site located at ambient pressure can grow at elevated pressures associated with Europa\u2019s upper ocean.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2025.1639438","type":"journal-article","created":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T15:59:04Z","timestamp":1770911944000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Microbes from ambient-pressure analogues offer insights into possible life in Europa\u2019s high-pressure subsurface ocean"],"prefix":"10.3389","volume":"16","author":[{"given":"Alvaro","family":"del Moral","sequence":"first","affiliation":[{"name":"AstrobiologyOU, School of Environment, Earth and Ecosystem Sciences, Open University","place":["Milton Keynes, United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominic","family":"Siggs","sequence":"additional","affiliation":[{"name":"AstrobiologyOU, School of Environment, Earth and Ecosystem Sciences, Open University","place":["Milton Keynes, United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael C.","family":"Macey","sequence":"additional","affiliation":[{"name":"AstrobiologyOU, School of Environment, Earth and Ecosystem Sciences, Open University","place":["Milton Keynes, United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark G.","family":"Fox-Powell","sequence":"additional","affiliation":[{"name":"AstrobiologyOU, School of Environment, Earth and Ecosystem Sciences, Open University","place":["Milton Keynes, United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victoria K.","family":"Pearson","sequence":"additional","affiliation":[{"name":"AstrobiologyOU, School of Physical Sciences, Open University","place":["Milton Keynes, United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen","family":"Olsson-Francis","sequence":"additional","affiliation":[{"name":"AstrobiologyOU, School of Environment, Earth and Ecosystem Sciences, Open University","place":["Milton Keynes, United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,2,12]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1007\/978-94-017-9918-8_18","article-title":"Effects of high hydrostatic pressure on microbial cell membranes: structural and functional perspectives","volume-title":"High pressure bioscience: basic concepts, applications and frontiers","author":"Abe","year":"2015"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1007\/BF00689356","article-title":"Inhibition of methanogenesis by sulphate reducing bacteria competing for transferred hydrogen","volume":"117","author":"Abram","year":"1978","journal-title":"Arch. 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