{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T03:29:39Z","timestamp":1790220579647,"version":"4.1.0"},"reference-count":48,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T00:00:00Z","timestamp":1757894400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p><jats:italic>Yersinia enterocolitica<\/jats:italic> is a significant foodborne Gram-negative pathogen causing gastroenteritis and systemic infections. Its virulence is attributed to its ability to withstand oxidative stress and evade host immunity. This study investigates the role of the sodA gene, which encodes manganese-dependent superoxide dismutase (Mn-SOD), in the pathogenicity of <jats:italic>Y. enterocolitica<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using sodA knockout (\u0394<jats:italic>sodA<\/jats:italic>) and complemented (\u0394<jats:italic>sodA<jats:sup>C<\/jats:sup><\/jats:italic>) strains, we assessed bacterial adhesion and invasion in Caco-2 epithelial cells, intracellular survival in RAW264.7 macrophages, and colonization in BALB\/c mice. Inflammatory responses were evaluated through histopathology, immunohistochemistry (NF-\u03baB p65), and qRT-PCR analysis of cytokine expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The \u0394<jats:italic>sodA<\/jats:italic> mutant exhibited significantly reduced adhesion to and invasion of epithelial cells, impaired survival within macrophages, and decreased colonization in murine ileum and colon tissues. Furthermore, \u0394<jats:italic>sodA<\/jats:italic> infection resulted in attenuated inflammatory responses, evidenced by lower expression of IL-1\u03b2, TNF-\u03b1, and NF-\u03baB p65. Functional restoration was observed in the complemented strain, confirming the specific role of sodA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>These results demonstrate that sodA is essential for the full virulence of Y. enterocolitica, influencing oxidative stress resistance, host cell invasion, and modulation of immune responses. This study highlights sodA as a potential target for developing therapeutic strategies against <jats:italic>Y. enterocolitica<\/jats:italic> infections.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2025.1643172","type":"journal-article","created":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T05:28:51Z","timestamp":1757914131000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["sodA modulates in vitro and in vivo virulence of Yersinia enterocolitica"],"prefix":"10.3389","volume":"16","author":[{"given":"Yingying","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodong","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinru","family":"Meng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruixue","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,9,15]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"5575","DOI":"10.1099\/ijsem.0.001485","article-title":"Genome-based phylogeny and taxonomy of the \u201cEnterobacteriales\u201d: proposal for Enterobacterales Ord. 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