{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T04:41:37Z","timestamp":1760589697075,"version":"build-2065373602"},"reference-count":48,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:00:00Z","timestamp":1760572800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>The emergence of multi-drug resistant <jats:italic>Shewanella xiamenensis<\/jats:italic> poses a significant threat to aquaculture and public health, driving the need for alternative therapies like bacteriophages.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A novel bacteriophage, SXP01, was isolated using S. xiamenensis A001 as a host. Its kinetic parameters (MOI, latent period) and stability across temperature (4-60\u00b0C), pH (6-11), and chloroform were characterized. The lysis cassette was identified genomically, and a recombinant endolysin (LysSXP-1) was expressed to test lytic activity. In vivo efficacy was evaluated in a crucian carp infection model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>SXP01, a Myoviridae member, showed an optimal MOI of 0.1, a 20-minute latent period, and high environmental stability. Its lysis cassette encodes two endolysins, with LysSXP-1 demonstrating direct lytic activity. In the fish model, SXP01 treatment significantly increased survival rates from 13.3% to 56.7%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>The study identifies SXP01 as a highly stable and effective bacteriophage. The synergistic action of its endolysins and its demonstrated in vivo efficacy highlight its strong potential as a targeted biocontrol agent to manage <jats:italic>S. xiamenensis<\/jats:italic> infections in aquaculture sustainably.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2025.1652450","type":"journal-article","created":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T04:17:18Z","timestamp":1760588238000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["SXP01: a novel bacteriophage for combating Shewanella xiamenensis in aquaculture"],"prefix":"10.3389","volume":"16","author":[{"given":"Chen","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yangjun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuyuan","family":"Peng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guofan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiajie","family":"Xie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinran","family":"Long","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linger","family":"Qian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yibo","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengbiao","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,10,16]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"5370","DOI":"10.1021\/acs.jpcb.0c03575","article-title":"Structural dynamics and topology of the inactive form of S21 holin in a lipid bilayer using continuous-wave electron paramagnetic resonance spectroscopy","volume":"124","author":"Ahammad","year":"2020","journal-title":"J. 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