{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T11:58:00Z","timestamp":1787313480103,"version":"build-2736575974"},"reference-count":120,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T00:00:00Z","timestamp":1756166400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Non-\n                    <jats:italic>Saccharomyces<\/jats:italic>\n                    wine yeasts have a promising role in biotechnological approaches to enhance wine complexity, particularly by influencing the aromatic profile.\n                    <jats:italic>Metschnikowia pulcherrima<\/jats:italic>\n                    is a non-\n                    <jats:italic>Saccharomyces<\/jats:italic>\n                    yeast that is notable for its antimicrobial activity and diverse enzymatic activities. These properties make this yeast a valid candidate for application as a starter culture in winemaking. This study evaluated the application of\n                    <jats:italic>M. pulcherrima 62<\/jats:italic>\n                    in sequential inoculation with\n                    <jats:italic>S. cerevisiae<\/jats:italic>\n                    for the production of Kallmet wine, delivered from the indigenous Kallmet grape variety traditionally cultivated in various regions of Albania. The use of different inoculation strategies resulted in significant differences in wine composition, affecting key oenological parameters, the aromatic profile and sensory attributes.\n                    <jats:italic>M. pulcherrima<\/jats:italic>\n                    62 did not negatively interfere with the fermentation kinetics of\n                    <jats:italic>S. cerevisiae<\/jats:italic>\n                    during fermentation and contributed to wines with lower amounts of ethanol and richer in glycerol, total polyphenols and anthocyanins. Moreover, increased levels of isobutanol, phenylethyl alcohol, isoamyl alcohol and monoterpenes (linalool, geraniol, and nerol) were observed in wines produced with\n                    <jats:italic>M. pulcherrima<\/jats:italic>\n                    62 and\n                    <jats:italic>S. cerevisiae<\/jats:italic>\n                    in sequential inoculation, compared to wines fermented solely with\n                    <jats:italic>S. cerevisiae<\/jats:italic>\n                    . Finally, sensory analysis revealed a distinct differentiation in the wines, attributable to the modulation of aromatic compounds by\n                    <jats:italic>M. pulcherrima<\/jats:italic>\n                    62.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2025.1654308","type":"journal-article","created":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T05:28:09Z","timestamp":1756186089000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Preliminary insights regarding the quality of Kallmet wine, obtained by sequential inoculation with Metschnikowia pulcherrima and Saccharomyces cerevisiae"],"prefix":"10.3389","volume":"16","author":[{"given":"Mamica","family":"Ruci","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata","family":"Kongoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Coppola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariantonietta","family":"Succi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Testa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Onejda","family":"Ky\u00e7yk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julian","family":"Karaulli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatbardha","family":"Lam\u00e7e","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Iorizzo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,8,26]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"302","DOI":"10.3390\/fermentation9030302","article-title":"Metschnikowia pulcherrima in cold clarification: biocontrol activity and aroma enhancement in Verdicchio wine","volume":"9","author":"Agarbati","year":"2023","journal-title":"Fermentation"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"126","DOI":"10.3390\/fermentation7030126","article-title":"Chemical and sensory profiles of merlot wines produced by sequential inoculation of Metschnikowia pulcherrima or Meyerzyma guilliermondii","volume":"7","author":"Aplin","year":"2021","journal-title":"Fermentation"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"1659","DOI":"10.3390\/microorganisms12081659","article-title":"Two-stage screening of Metschnikowia spp. bioprotective properties: from grape juice to fermented must by Saccharomyces cerevisiae","volume":"12","author":"Aragno","year":"2024","journal-title":"Microorganisms"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"130081","DOI":"10.1016\/j.foodchem.2021.130081","article-title":"Insights on the role of acetaldehyde and other aldehydes in the odour and tactile nasal perception of red wine","volume":"361","author":"Arias-P\u00e9rez","year":"2021","journal-title":"Food Chem."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"2700","DOI":"10.1021\/jf026115z","article-title":"Prediction of aged red wine aroma properties from aroma chemical composition. 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Int."},{"key":"ref83","doi-asserted-by":"publisher","first-page":"135","DOI":"10.20870\/oeno-one.2018.52.2.2062","article-title":"Polyphenolic profile and stilbene content of Albanian \u201cKallmet\u201d monovarietal wine","volume":"52","author":"Pe\u00e7uli","year":"2018","journal-title":"OENO One"},{"key":"ref84","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1016\/S0308-8146(03)00282-6","article-title":"Comparative study of aromatic compounds in two young white wines subjected to pre-fermentative cryomaceration","volume":"84","author":"Peinado","year":"2004","journal-title":"Food Chem."},{"key":"ref85","doi-asserted-by":"publisher","first-page":"785","DOI":"10.3390\/fermentation9090785","article-title":"Sequential inoculation of Metschnikowia pulcherrima and Saccharomyces cerevisiae as a biotechnological tool to increase the terpenes content of pecorino white wines","volume":"9","author":"Perpetuini","year":"2023","journal-title":"Fermentation"},{"key":"ref86","doi-asserted-by":"publisher","first-page":"1405","DOI":"10.1007\/s00217-020-03499-8","article-title":"Contribution of Pichia manshurica strains to aroma profile of organic wines","volume":"246","author":"Perpetuini","year":"2020","journal-title":"Eur. 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Technol."},{"key":"ref100","doi-asserted-by":"publisher","first-page":"88","DOI":"10.3390\/beverages10030088","article-title":"Preliminary characterisation of Metschnikowia pulcherrima to be used as a starter culture in red winemaking","volume":"10","author":"Testa","year":"2024","journal-title":"Beverages"},{"key":"ref101","doi-asserted-by":"publisher","first-page":"326","DOI":"10.3390\/pr9020326","article-title":"Influence of <i>Hanseniaspora uvarum<\/i> AS27 on chemical and sensorial characteristics of Aglianico wine","volume":"9","author":"Testa","year":"2021","journal-title":"Processes"},{"key":"ref102","doi-asserted-by":"publisher","first-page":"159","DOI":"10.3390\/fermentation11030159","article-title":"Biotechnological strategies for ethanol reduction in wine","volume":"11","author":"Testa","year":"2025","journal-title":"Fermentation"},{"key":"ref103","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1007\/s00217-019-03424-8","article-title":"Use of strain Hanseniaspora guilliermondii BF1 for winemaking process of white grapes Vitis vinifera cv Fiano","volume":"246","author":"Testa","year":"2020","journal-title":"Eur. 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Forum"},{"key":"ref115","doi-asserted-by":"publisher","first-page":"127795","DOI":"10.1016\/j.foodchem.2020.127795","article-title":"Targeted metabolomics of anthocyanin derivatives during prolonged wine aging: evolution, color contribution and aging prediction","volume":"339","author":"Zhang","year":"2021","journal-title":"Food Chem."},{"key":"ref116","doi-asserted-by":"publisher","first-page":"606","DOI":"10.3389\/fmicb.2018.00606","article-title":"Use of Torulaspora delbrueckii co-fermentation with two Saccharomyces cerevisiae strains with different aromatic characteristic to improve the diversity of red wine aroma profile","volume":"9","author":"Zhang","year":"","journal-title":"Front. 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