{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T18:07:15Z","timestamp":1787940435791,"version":"build-2784847793"},"reference-count":95,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T00:00:00Z","timestamp":1764892800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Human staphylococcal food poisoning is caused by a range of heat-stable staphylococcal enterotoxins that are released into food by\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    . Additionally, antimicrobial resistance has steadily grown into a significant global issue that endangers food safety and human health. Therefore, this study aimed to determine the frequency of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    in foods sold on the streets of Mangaung Metropolitan Municipality, South Africa, and thoroughly examine the isolated strains\u2019 genetic traits, virulence, and antibiotic resistance profiles. Out of 168 samples, which included salad, pap (maize meal), chicken, and pork, all (100%) samples showed the occurrence of\n                    <jats:italic>Staphylococcus<\/jats:italic>\n                    species. Furthermore, 29.7% of isolates were subsequently identified by MALDI-TOF MS as\n                    <jats:italic>S. aureus<\/jats:italic>\n                    . Moreover, antimicrobial susceptibility testing of 50\n                    <jats:italic>S. aureus<\/jats:italic>\n                    isolates showed that 42% were resistant to penicillin, followed by cefoxitin at 46% and ciprofloxacin at 44%. The multidrug-resistant (MDR) profile revealed that 52% of the isolates were resistant to three or more classes of antibiotics. Additionally, four sequenced isolates were identified by in silico MLST as having sequence types (STs) 243, although strain SVF3 contained a unique ST designated as * ff2b. All four isolates were identified as belonging to Staphylococcal Protein A type (\n                    <jats:italic>spa-type<\/jats:italic>\n                    ) t21 by whole genome sequencing. All sequenced isolates exhibited a total of 9 antibiotic resistance genes and 63 virulence genes. The current study showed the importance of monitoring for high virulence potential and antimicrobial resistance of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    in retail food and increasing awareness of potential risk for such strains; furthermore, infection control measures, antimicrobial stewardship, and periodic One Health epidemiological surveillance studies are needed to monitor and contain the threat of increasing antibiotic resistance in Africa.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2025.1669035","type":"journal-article","created":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T06:28:55Z","timestamp":1764916135000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Antimicrobial resistance and genomic characterization of Staphylococcus aureus in ready-to-eat foods from Mangaung Metro Municipality"],"prefix":"10.3389","volume":"16","author":[{"given":"Pontso","family":"Letuka","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebolelo J.","family":"Nkhebenyane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsepo","family":"Ramatla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tywabi-Ngeva","family":"Zikhona","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kgaugelo E.","family":"Lekota","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ntelekwane G.","family":"Khasapane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,12,5]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1007\/978-3-031-62839-9_18","article-title":"New discoveries in toxins from gram-positive bacteria staphylococcus aureus","volume-title":"Microbial toxins in food systems: Causes, mechanisms, complications, and metabolism","author":"Abdallah","year":"2024"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"1276","DOI":"10.3390\/pathogens10101276","article-title":"Molecular characterization of Staphylococcus aureus isolated from human and food samples in northern Algeria","volume":"10","author":"Achek","year":"2021","journal-title":"Pathogens"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"426","DOI":"10.1186\/s12866-024-03575-z","article-title":"Whole genome sequence analysis of multi-drug resistant and biofilm-forming Staphylococcus haemolyticus isolated from bovine milk","volume":"24","author":"Ajose","year":"2024","journal-title":"BMC Microbiol."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"893","DOI":"10.1007\/s13668-023-00510-y","article-title":"A scoping review of studies evaluating the healthiness of street food vendors","volume":"12","author":"Akhter","year":"2023","journal-title":"Curr. Nutr. Rep."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/j.clinms.2020.03.001","article-title":"Determination of methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis by MALDI-TOF MS in clinical isolates from Latvia","volume":"16","author":"Alksne","year":"2020","journal-title":"Clin. Mass Spectrom."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1186\/s12866-019-1509-4","article-title":"Bacteriological profile, antimicrobial susceptibility patterns of the isolates among street vended foods and hygienic practice of vendors in Gondar town, Northwest Ethiopia: a cross sectional study","volume":"19","author":"Amare","year":"2019","journal-title":"BMC Microbiol."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"1487","DOI":"10.3390\/ijerph120201487","article-title":"Multidrug efflux pumps from Enterobacteriaceae, vibrio cholerae and Staphylococcus aureus bacterial food pathogens","volume":"12","author":"Andersen","year":"2015","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"94","DOI":"10.7506\/spkx1002-6630-20220822-256","article-title":"Toxin-encoding genes and drug susceptibility of Staphylococcus aureus from vegetables consumed raw","volume":"44","author":"Bai","year":"2023","journal-title":"Food Sci."},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuae002","article-title":"The role of human extracellular matrix proteins in defining Staphylococcus aureus biofilm infections","volume":"48","author":"Bhattacharya","year":"2024","journal-title":"FEMS Microbiol. Rev."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"101147","DOI":"10.1016\/j.drup.2024.101147","article-title":"Mechanism of staphylococcal resistance to clinically relevant antibiotics","volume":"77","author":"Brdov\u00e1","year":"2024","journal-title":"Drug Resist. Updat."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"e0134592","DOI":"10.1371\/journal.pone.0134592","article-title":"Extensive genomic diversity among bovine-adapted Staphylococcus aureus: evidence for a genomic rearrangement within CC97","volume":"10","author":"Budd","year":"2015","journal-title":"PLoS One"},{"key":"ref12","doi-asserted-by":"publisher","first-page":"590","DOI":"10.3168\/jds.2017-13226","article-title":"Evaluation of MALDI-TOF mass spectrometry and a custom reference spectra expanded database for the identification of bovine-associated coagulase-negative staphylococci","volume":"101","author":"Cameron","year":"2018","journal-title":"J. Dairy Sci."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"3895","DOI":"10.1128\/aac.02412-14","article-title":"In silico detection and typing of plasmids using PlasmidFinder and plasmid multilocus sequence typing","volume":"58","author":"Carattoli","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref14","first-page":"213","article-title":"Staphylococcus aureus, a food pathogen: virulence factors and antibiotic resistance","volume-title":"Foodborne diseases","author":"Castro","year":"2018"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"1038","DOI":"10.1016\/j.trac.2010.06.004","article-title":"Fast screening methods to detect antibiotic residues in food samples","volume":"29","author":"Ch\u00e1fer-Peric\u00e1s","year":"2010","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref16","doi-asserted-by":"publisher","first-page":"1925","DOI":"10.1093\/bioinformatics\/btz848","article-title":"GTDB-Tk: a toolkit to classify genomes with the genome taxonomy database","volume":"36","author":"Chaumeil","year":"2020","journal-title":"Bioinformatics"},{"key":"ref17","doi-asserted-by":"publisher","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"Fastp: an ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"ref18","volume-title":"Performance standards for antimicrobial testing, CLSI supplement M100","year":"2024"},{"key":"ref19","doi-asserted-by":"publisher","first-page":"59","DOI":"10.2174\/1874285801307010059","article-title":"Multidrug efflux pumps in Staphylococcus aureus: an update","volume":"7","author":"Costa","year":"2013","journal-title":"Open Microbiol. 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Microbiol."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"e0287996","DOI":"10.1371\/journal.pone.0287996","article-title":"Food safety knowledge, attitude, and practice of street food vendors and associated factors in low-and middle-income countries: a systematic review and Meta-analysis","volume":"18","author":"Desye","year":"2023","journal-title":"PLoS One"},{"key":"ref24","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.micpath.2017.12.030","article-title":"Genetics of bi-component leukocidin and drug resistance in nasal and clinical Staphylococcus aureus in Lagos, Nigeria","volume":"115","author":"Enwuru","year":"2018","journal-title":"Microb. Pathog."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"e1319","DOI":"10.7717\/peerj.1319","article-title":"Anvi\u2019o: an advanced analysis and visualization platform for \u2018omics data","volume":"3","author":"Eren","year":"2015","journal-title":"PeerJ"},{"key":"ref26","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1016\/S0168-1605(02)00172-1","article-title":"Microbiological quality of 18 C ready-to-eat food products sold in Taiwan","volume":"80","author":"Fang","year":"2003","journal-title":"Int. J. Food Microbiol."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"5304","DOI":"10.1093\/bioinformatics\/btaa1051","article-title":"Deepnog: fast and accurate protein orthologous group assignment","volume":"36","author":"Feldbauer","year":"2020","journal-title":"Bioinformatics"},{"key":"ref28","doi-asserted-by":"publisher","first-page":"000832","DOI":"10.1099\/mgen.0.000832","article-title":"Curation of the AMRFinderPlus databases: applications, functionality and impact","volume":"8","author":"Feldgarden","year":"2022","journal-title":"Microb. Genomics"},{"key":"ref29","doi-asserted-by":"publisher","first-page":"e00162","DOI":"10.1128\/jb.00162-22","article-title":"Revisiting the role of VraTSR in Staphylococcus aureus response to cell wall-targeting antibiotics","volume":"204","author":"Fernandes","year":"2022","journal-title":"J. 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Rev."},{"key":"ref32","doi-asserted-by":"publisher","first-page":"427","DOI":"10.3390\/antibiotics11040427","article-title":"Antimicrobial susceptibility testing: a comprehensive review of currently used methods","volume":"11","author":"Gajic","year":"2022","journal-title":"Antibiotics"},{"key":"ref33","doi-asserted-by":"publisher","first-page":"e77195","DOI":"10.7554\/eLife.77195","article-title":"Niche-specific genome degradation and convergent evolution shaping Staphylococcus aureus adaptation during severe infections","volume":"11","author":"Giulieri","year":"2022","journal-title":"eLife"},{"key":"ref34","doi-asserted-by":"publisher","first-page":"1072","DOI":"10.1093\/bioinformatics\/btt086","article-title":"QUAST: quality assessment tool for genome assemblies","volume":"29","author":"Gurevich","year":"2013","journal-title":"Bioinformatics"},{"key":"ref35","doi-asserted-by":"publisher","first-page":"e01876-24","DOI":"10.1128\/aem.01876-24","article-title":"CARPDM: cost-effective antibiotic resistome profiling of metagenomic samples using targeted enrichment","volume":"91","author":"Hackenberger","year":"2025","journal-title":"Appl. Environ. Microbiol."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1128\/mbio.01434-16","article-title":"Amino acid catabolism in Staphylococcus aureus and the function of carbon catabolite repression","volume":"8","author":"Halsey","year":"2017","journal-title":"MBio"},{"key":"ref37","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1108\/17590831111139839","article-title":"Consumption of functional food model for Malay Muslims in Malaysia","volume":"2","author":"Hasnah Hassan","year":"2011","journal-title":"J. Islam. Mark."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1186\/1471-2105-11-119","article-title":"Prodigal: prokaryotic gene recognition and translation initiation site identification","volume":"11","author":"Hyatt","year":"2010","journal-title":"BMC Bioinformatics."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"e42107","DOI":"10.1016\/j.heliyon.2025.e42107","article-title":"Prevalence, antibiotic resistance patterns, and virulence factors of Staphylococcus aureus isolates associated with bovine mastitis in northern Bangladesh","volume":"11","author":"Islam","year":"2025","journal-title":"Heliyon."},{"key":"ref40","volume-title":"Microbiology of the food chain-horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species)-part 1: Method using Baird-Parker agar medium.","year":"2021"},{"key":"ref41","doi-asserted-by":"publisher","first-page":"154","DOI":"10.3390\/ani14010154","article-title":"Detection of staphylococcus isolates and their antimicrobial resistance profiles and virulence genes from subclinical mastitis cattle milk using MALDI-TOF MS, PCR and sequencing in free state province, South Africa","volume":"14","author":"Khasapane","year":"2024","journal-title":"Animals"},{"key":"ref42","doi-asserted-by":"publisher","first-page":"1376620","DOI":"10.3389\/fmicb.2024.1376620","article-title":"Comprehensive whole genome analysis of Staphylococcus aureus isolates from dairy cows with subclinical mastitis","volume":"15","author":"Khasapane","year":"2024","journal-title":"Front. Microbiol."},{"key":"ref43","doi-asserted-by":"publisher","first-page":"540","DOI":"10.1038\/s41587-019-0072-8","article-title":"Assembly of long, error-prone reads using repeat graphs","volume":"37","author":"Kolmogorov","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"24","DOI":"10.51585\/gjvr.2023.1.0049","article-title":"Antimicrobial resistance of Salmonella strains isolated from food products of animal origin in Ukraine between 2018-2021","volume":"3","author":"Kozytska","year":"2023","journal-title":"Ger. J. Vet. Res."},{"key":"ref45","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1128\/aem.46.1.165-170.1983","article-title":"Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of fecal contamination of foods","volume":"46","author":"Krumperman","year":"1983","journal-title":"Appl. Environ. Microbiol."},{"key":"ref46","doi-asserted-by":"publisher","first-page":"1639","DOI":"10.1101\/gr.092759.109","article-title":"Circos: an information aesthetic for comparative genomics","volume":"19","author":"Krzywinski","year":"2009","journal-title":"Genome Res."},{"key":"ref47","doi-asserted-by":"publisher","first-page":"986","DOI":"10.1186\/s12864-024-10906-x","article-title":"Genomic insight on Klebsiella variicola isolated from wastewater treatment plant has uncovered a novel bacteriophage","volume":"25","author":"Lekota","year":"2024","journal-title":"BMC Genomics"},{"key":"ref48","doi-asserted-by":"publisher","first-page":"392","DOI":"10.3389\/fmicb.2019.00392","article-title":"Tetracycline gene transfer in Staphylococcus xylosus in situ during sausage fermentation","volume":"10","author":"Leroy","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref49","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1108\/BFJ-07-2020-0595","article-title":"Street food handlers' food safety knowledge, attitudes and self-reported practices and consumers' perceptions about street food vending in Maseru, Lesotho","volume":"123","author":"Letuka","year":"2021","journal-title":"Br. Food J."},{"key":"ref50","doi-asserted-by":"publisher","first-page":"524","DOI":"10.3390\/biom9100524","article-title":"The prevalence, antibiotic resistance and biofilm formation of Staphylococcus aureus in bulk ready-to-eat foods","volume":"9","author":"Lin","year":"2019","journal-title":"Biomolecules"},{"key":"ref51","doi-asserted-by":"publisher","first-page":"608","DOI":"10.4315\/0362-028X-72.3.608","article-title":"Staphylococcus aureus isolated from pork and chicken carcasses in Taiwan: prevalence and antimicrobial susceptibility","volume":"72","author":"Lin","year":"2009","journal-title":"J. Food Prot."},{"key":"ref52","doi-asserted-by":"publisher","first-page":"D912","DOI":"10.4315\/0362-028X.JFP-18-574","article-title":"VFDB 2022: a general classification scheme for bacterial virulence factors","volume":"50","author":"Liu","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref53","doi-asserted-by":"publisher","first-page":"105649","DOI":"10.1016\/j.meegid.2024.105649","article-title":"A genome-based investigation of the Priestia species isolated from anthrax endemic regions in Kruger national park","volume":"123","author":"Magome","year":"2024","journal-title":"Infect. Genet. Evol."},{"key":"ref54","doi-asserted-by":"publisher","first-page":"109165","DOI":"10.1016\/j.ijfoodmicro.2021.109165","article-title":"Multidrug-, methicillin-, and vancomycin-resistant Staphylococcus aureus isolated from ready-to-eat meat sandwiches: an ongoing food and public health concern","volume":"346","author":"Mahros","year":"2021","journal-title":"Int. J. Food Microbiol."},{"key":"ref55","doi-asserted-by":"publisher","first-page":"436","DOI":"10.12691\/jfnr-5-6-11","article-title":"Assessment of aerobic plate counts, Staphylococcus aureus, Escherichia coli and Salmonella in meat sold by street vendors in the eastern Cape Province, South Africa","volume":"5","author":"Mazizi","year":"2017","journal-title":"J. Food Nutr. Res."},{"key":"ref56","doi-asserted-by":"publisher","first-page":"2182","DOI":"10.1038\/s41467-019-10210-3","article-title":"TYGS is an automated high-throughput platform for state-of-the-art genome-based taxonomy","volume":"10","author":"Meier-Kolthoff","year":"2019","journal-title":"Nat. Commun."},{"key":"ref57","doi-asserted-by":"publisher","first-page":"835","DOI":"10.3390\/toxins13120835","article-title":"Prevalence, enterotoxigenic potential and antimicrobial resistance of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) isolated from Algerian ready to eat foods","volume":"13","author":"Mekhloufi","year":"2021","journal-title":"Toxins"},{"key":"ref58","doi-asserted-by":"publisher","first-page":"e01083","DOI":"10.1128\/msystems.01083-21","article-title":"Species-scale genomic analysis of Staphylococcus aureus genes influencing phage host range and their relationships to virulence and antibiotic resistance genes","volume":"7","author":"Moller","year":"2022","journal-title":"MSystems."},{"key":"ref59","doi-asserted-by":"publisher","first-page":"2040","DOI":"10.1186\/s12889-024-19589-1","article-title":"Unlocking food safety: a comprehensive review of South Africa\u2019s food control and safety landscape from an environmental health perspective","volume":"24","author":"Mphaga","year":"2024","journal-title":"BMC Public Health"},{"key":"ref60","doi-asserted-by":"publisher","first-page":"18994","DOI":"10.1038\/s41598-021-98432-8","article-title":"Comparative analysis of prophages carried by human and animal-associated Staphylococcus aureus strains spreading across the European regions","volume":"11","author":"Naorem","year":"2021","journal-title":"Sci. Rep."},{"key":"ref61","doi-asserted-by":"publisher","first-page":"1422902","DOI":"10.3389\/fmicb.2024.1422902","article-title":"Investigating genomic diversity of Staphylococcus aureus associated with pediatric atopic dermatitis in South Africa","volume":"15","author":"Ndhlovu","year":"2024","journal-title":"Front. Microbiol."},{"key":"ref62","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/j.foodcont.2014.04.026","article-title":"Investigation of the food value chain of ready-to-eat chicken and the associated risk for staphylococcal food poisoning in Tshwane metropole, South Africa","volume":"45","author":"Oguttu","year":"2014","journal-title":"Food Control"},{"key":"ref63","doi-asserted-by":"publisher","first-page":"3691","DOI":"10.1093\/bioinformatics\/btv421","article-title":"Roary: rapid large-scale prokaryote pan genome analysis","volume":"31","author":"Page","year":"2015","journal-title":"Bioinformatics"},{"key":"ref64","doi-asserted-by":"publisher","first-page":"3970","DOI":"10.1038\/s41467-020-17735-y","article-title":"Efflux pump activity potentiates the evolution of antibiotic resistance across S. aureus isolates","volume":"11","author":"Papkou","year":"2020","journal-title":"Nat. Commun."},{"key":"ref65","doi-asserted-by":"publisher","first-page":"1043","DOI":"10.1101\/gr.186072.114","article-title":"CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes","volume":"25","author":"Parks","year":"2015","journal-title":"Genome Res."},{"key":"ref66","doi-asserted-by":"publisher","first-page":"2223","DOI":"10.3390\/ijerph15102223","article-title":"Antimicrobial resistance profiles of Staphylococcus aureus isolated from meat carcasses and bovine milk in abattoirs and dairy farms of the eastern cape, South Africa","volume":"15","author":"Pekana","year":"2018","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"ref9001","doi-asserted-by":"publisher","first-page":"1702","DOI":"10.3390\/antibiotics11121702","article-title":"PurN is involved in antibiotic tolerance and virulence in Staphylococcus aureus","volume":"11","author":"Peng","year":"2022","journal-title":"Antibiotics."},{"key":"ref67","doi-asserted-by":"publisher","first-page":"e00751","DOI":"10.1128\/msphere.00751-23","article-title":"Evolutionary dynamics of the accessory genomes of Staphylococcus aureus","volume":"9","author":"Piper","year":"2024","journal-title":"Msphere."},{"key":"ref68","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/978-3-030-83251-3_2","article-title":"Bacterial adhesion, virulence, and biofilm formation","volume-title":"Musculoskeletal infection","author":"Pugazhendhi","year":"2022"},{"key":"ref69","doi-asserted-by":"publisher","first-page":"995","DOI":"10.3389\/fimmu.2019.00995","article-title":"PSM peptides from community-associated methicillin-resistant Staphylococcus aureus impair the adaptive immune response via modulation of dendritic cell subsets in vivo","volume":"10","author":"Richardson","year":"2019","journal-title":"Front. Immunol."},{"key":"ref70","doi-asserted-by":"publisher","first-page":"1344","DOI":"10.3389\/fmicb.2017.01344","article-title":"Detection and characterization of Staphylococcus aureus and methicillin-resistant S. aureus in foods confiscated in EU borders","volume":"8","author":"Rodr\u00edguez-L\u00e1zaro","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref71","doi-asserted-by":"publisher","first-page":"882","DOI":"10.3389\/fmicb.2015.00882","article-title":"Staphylococcus aureus strains associated with food poisoning outbreaks in France: comparison of different molecular typing methods, including MLVA","volume":"6","author":"Roussel","year":"2015","journal-title":"Front. Microbiol."},{"key":"ref72","doi-asserted-by":"publisher","first-page":"136","DOI":"10.21608\/bvmj.2023.224548.1688","article-title":"Staphylococcus aureus incidence in Egyptian meat outlets and butcheries, and their biofilm, antibiotic-resistance, and virulence capabilities","volume":"45","author":"Saad","year":"2023","journal-title":"Benha Vet. Med. J."},{"key":"ref73","doi-asserted-by":"publisher","first-page":"3774","DOI":"10.3390\/foods12203774","article-title":"The socioeconomic factors of street food vending in developing countries and its implications for public health: a systematic review","volume":"12","author":"Salamandane","year":"2023","journal-title":"Foods"},{"key":"ref9002","doi-asserted-by":"publisher","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","article-title":"Prokka: rapid prokaryotic genome annotation","volume":"30","author":"Seemann","year":"2014","journal-title":"Bioinform."},{"key":"ref74","doi-asserted-by":"publisher","first-page":"13","DOI":"10.21608\/bvmj.2017.31106","article-title":"Incidence of coliform and Staphylococcus aureus in ready to eat fast foods","volume":"32","author":"Shaltout","year":"2017","journal-title":"Benha Vet. Med. 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J."},{"key":"ref77","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1016\/j.fm.2018.06.006","article-title":"Modeling the effect of pH, water activity, and ethanol concentration on biofilm formation of Staphylococcus aureus","volume":"76","author":"Tango","year":"2018","journal-title":"Food Microbiol."},{"key":"ref78","doi-asserted-by":"publisher","first-page":"e15824","DOI":"10.7717\/peerj.15824","article-title":"Prevalence and virulence genes of Staphylococcus aureus from food contact surfaces in Thai restaurants","volume":"11","author":"Tasanapak","year":"2023","journal-title":"PeerJ."},{"key":"ref79","doi-asserted-by":"publisher","first-page":"6614","DOI":"10.1093\/nar\/gkw569","article-title":"NCBI prokaryotic genome annotation pipeline","volume":"44","author":"Tatusova","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref80","doi-asserted-by":"publisher","first-page":"141","DOI":"10.14202\/IJOH.2023.141-147","article-title":"Staphylococcus aureus as a foodborne pathogen in eggs and egg products in Indonesia: a review","volume":"10","author":"Thaha","year":"2024","journal-title":"Int. J. One Health."},{"key":"ref81","doi-asserted-by":"publisher","first-page":"1211","DOI":"10.3390\/microorganisms10061211","article-title":"Antimicrobial resistance, enterotoxin and mec gene profiles of Staphylococcus aureus associated with beef-based protein sources from KwaZulu-Natal province, South Africa","volume":"10","author":"Thwala","year":"2022","journal-title":"Microorganisms"},{"key":"ref82","doi-asserted-by":"publisher","first-page":"6761","DOI":"10.1093\/nar\/gkv657","article-title":"Microbial species delineation using whole genome sequences","volume":"43","author":"Varghese","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"ref83","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1016\/j.diagmicrobio.2004.03.009","article-title":"Prevalence of genes encoding for members of the staphylococcal leukotoxin family among clinical isolates of Staphylococcus aureus","volume":"49","author":"von Eiff","year":"2004","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref84","doi-asserted-by":"publisher","first-page":"2256","DOI":"10.3389\/fmicb.2017.02256","article-title":"Enterotoxigenicity and antimicrobial resistance of Staphylococcus aureus isolated from retail food in China","volume":"8","author":"Wang","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref85","doi-asserted-by":"publisher","first-page":"2577","DOI":"10.3389\/fmicb.2019.02577","article-title":"Diversity of serotype, genotype, and antibiotic susceptibility of Salmonella prevalent in pickled ready-to-eat meat","volume":"10","author":"Wang","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref86","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1186\/1471-2164-7-112","article-title":"Conserved genes in a path from commensalism to pathogenicity: comparative phylogenetic profiles of Staphylococcus epidermidis RP62A and ATCC12228","volume":"7","author":"Wei","year":"2006","journal-title":"BMC Genomics"},{"key":"ref87","volume-title":"Global antimicrobial resistance and use surveillance system (GLASS) report 2022","year":"2022"},{"key":"ref88","doi-asserted-by":"publisher","first-page":"2767","DOI":"10.3389\/fmicb.2018.02767","article-title":"Staphylococcus aureus isolated from retail meat and meat products in China: incidence, antibiotic resistance and genetic diversity","volume":"9","author":"Wu","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref89","doi-asserted-by":"publisher","first-page":"331","DOI":"10.4315\/0362-028X.JFP-13-301","article-title":"Prevalence, antimicrobial susceptibility, and enterotoxin gene detection of Staphylococcus aureus isolates in ready-to-eat foods in Shaanxi, People's Republic of China","volume":"77","author":"Xing","year":"2014","journal-title":"J. Food Prot."},{"key":"ref90","doi-asserted-by":"publisher","first-page":"816","DOI":"10.3389\/fmicb.2016.00816","article-title":"Prevalence of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus in retail ready-to-eat foods in China","volume":"7","author":"Yang","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref91","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1016\/j.imlet.2013.01.004","article-title":"Staphylococcus aureus virulence factors in evasion from innate immune defenses in human and animal diseases","volume":"150","author":"Zecconi","year":"2013","journal-title":"Immunol. Lett."},{"key":"ref92","doi-asserted-by":"publisher","first-page":"1876","DOI":"10.3389\/fmicb.2018.01876","article-title":"Association between agr type, virulence factors, biofilm formation and antibiotic resistance of Staphylococcus aureus isolates from pork production","volume":"9","author":"Zhang","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref93","doi-asserted-by":"publisher","first-page":"1582","DOI":"10.1038\/s42003-024-07216-z","article-title":"An amphipathic peptide combats multidrug-resistant Staphylococcus aureus and biofilms","volume":"7","author":"Zhang","year":"2024","journal-title":"Commun. 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