{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T06:11:37Z","timestamp":1759817497086,"version":"build-2065373602"},"reference-count":85,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Dietary prebiotic oligosaccharides, complex carbohydrates that support beneficial bacteria, are ubiquitous on marketplace shelves and in people\u2019s diets. Though widely accessible and consumed, little is known about how different prebiotics alter the epithelium and microbes during enteric infections.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Here we show two structurally different prebiotic oligosaccharides, human milk oligosaccharides (HMO) and mannanoligosaccharides (MOS), alter the metabolism of colonic epithelial cells and <jats:italic>Salmonella enterica<\/jats:italic> sv. Typhimurium in ways specific to each prebiotic during infection in a focused \u2018<jats:italic>in vitro<\/jats:italic>\u2019 model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Initially, HMO and MOS addition decreased <jats:italic>S<\/jats:italic>. Typhimurium association with epithelial cells. However, gene expression analysis revealed significantly induced expression of Specific Pathogenicity Island (SPI) 1 (adj. <jats:italic>p<\/jats:italic> &amp;lt; 2.0\u22126) and 2 (adj. <jats:italic>p<\/jats:italic> &amp;lt; 3.0\u22125) with HMO treatment, opposed to increased fimbriae expression (adj.<jats:italic>p<\/jats:italic> &amp;lt; 3.0\u22123) with MOS treatment. Both host and pathogen metabolism were likewise altered with prebiotic addition. MOS treatment induced the expression of genes for amino acid metabolism in both the host cells and in <jats:italic>S<\/jats:italic>. Typhimurium, a metabolic shift that was not observed in the HMO treated cells. MOS treatment also altered pathogen-related respiration metabolism in <jats:italic>S<\/jats:italic>. Typhimurium toward activity typically seen during gut inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>The regulation of virulence expression in <jats:italic>Salmonella<\/jats:italic> from prebiotic treatment was unexpected and suggests prebiotics act in context-dependent ways to potentiate or attenuate enteric activity.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmicb.2025.1672770","type":"journal-article","created":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T05:35:19Z","timestamp":1759815319000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Cellular respiration and amino acid metabolism is altered by dietary oligosaccharides in Salmonella with epithelial cell association"],"prefix":"10.3389","volume":"16","author":[{"given":"Claire A.","family":"Shaw","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Poyin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Narine","family":"Arabyan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bart C.","family":"Weimer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,10,7]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"4453","DOI":"10.4049\/jimmunol.174.8.4453","article-title":"TLR signaling in the gut in health and disease","volume":"174","author":"Abreu","year":"2005","journal-title":"J. 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