{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T18:42:21Z","timestamp":1791225741051,"version":"4.1.0"},"reference-count":55,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T00:00:00Z","timestamp":1765411200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    <jats:italic>Enterococcus faecalis<\/jats:italic>\n                    is an opportunistic pathogen associated with nosocomial infections, food spoilage, and reduced efficacy of orally administered medications in patients with Parkinson\u2019s disease. Its genetic adaptability, particularly in acquiring virulence and antibiotic resistance genes, poses a significant challenge in treatment. \u0422hus, the development of new and effective approaches, such as phage therapy, is crucial in the fight against\n                    <jats:italic>E. faecalis<\/jats:italic>\n                    . The main goal of this study was to establish the biological characteristics of three bacteriophages (designated as vB_SEF_8, vB_SEF_13 and vB_SEF_15) isolated from wastewater in Bulgaria and their potential to eliminate\n                    <jats:italic>E. faecalis<\/jats:italic>\n                    . The host ranges of the phages were determined primarily using\n                    <jats:italic>E. faecalis<\/jats:italic>\n                    strains (\n                    <jats:italic>n<\/jats:italic>\n                    \u202f=\u202f29), although other species within the genus\n                    <jats:italic>Enterococcus<\/jats:italic>\n                    were also included. All three phages targeted only\n                    <jats:italic>E. faecalis<\/jats:italic>\n                    strains, including antibiotic-resistant or multidrug-resistant strains. The phages showed broad pH (4.0\u201310.5) and temperature (up to 80\u202f\u00b0C) stability, formed clear plaques, with maximal titers reached at various MOIs. After 9\u202fmonths at 4\u202f\u00b0C, only a slight titer reduction was observed (up to 2 log\n                    <jats:sub>10<\/jats:sub>\n                    PFU\/mL). RFLP analysis revealed genetic diversity among the three phage isolates. The phage with the broadest host range (vB_SEF_8) was characterized in more details. TEM observation revealed elongated head and long noncontractile tail. vB_SEF_8 possessed linear dsDNA and lacked genes associated with lysogeny, antibiotic resistance, or virulence. Phylogenetic analysis and the calculated pairwise intergenomic distance showed that vB_SEF_8 is a novel species within the\n                    <jats:italic>Saphexavirus<\/jats:italic>\n                    genus, class\n                    <jats:italic>Caudoviricetes<\/jats:italic>\n                    . The phage also successfully inhibited\n                    <jats:italic>E. faecalis<\/jats:italic>\n                    in a milk-based matrix. The collected data demonstrate that vB_SEF_8 holds significant potential as an antibacterial and therapeutic agent against\n                    <jats:italic>E. faecalis<\/jats:italic>\n                    in settings where the presence of this bacterium is undesirable.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2025.1674800","type":"journal-article","created":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T11:28:04Z","timestamp":1765452484000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Characterization of wastewater-derived bacteriophages infecting Enterococcus faecalis in Bulgaria: insights into the novel phage vB_SEF_8"],"prefix":"10.3389","volume":"16","author":[{"given":"Yoana","family":"Kizheva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsveta","family":"Dimova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Pandova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoana","family":"Gladicheva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralitsa","family":"Petrova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsvetelina","family":"Paunova-Krasteva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoltan","family":"Urshev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergei","family":"Ivanov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petya","family":"Hristova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,12,11]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1186\/s12866-025-03785-z","article-title":"Therapeutic potential of a newly isolated bacteriophage against multi-drug resistant Enterococcus faecalis infections: in vitro and in vivo characterization","volume":"25","author":"Ali","year":"2025","journal-title":"BMC Microbiol."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"e00512-19","DOI":"10.1128\/IAI.00512-19","article-title":"Identification of novel bacteriophages with therapeutic potential that target Enterococcus faecalis","volume":"87","author":"Al-Zubidi","year":"2019","journal-title":"Infect. 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