{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T23:40:28Z","timestamp":1785368428541,"version":"3.55.0"},"reference-count":254,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T00:00:00Z","timestamp":1771286400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Ancient cryospheric environments may preserve overlooked reservoirs of antimicrobial resistance (AMR) and bioactive potential. This study reports the first whole-genome sequencing and functional characterization of Psychrobacter sp. SC65A.3 isolated from 5,000-year-old ice from Sc\u0103ri\u0219oara Ice Cave, revealing a multidrug-resistance phenotype alongside antimicrobial activity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Whole-genome sequencing combined with phenotypic characterization for extremotolerance, antibiotic susceptibility and biochemical profile were used to identify and functionally characterize the ancient Psychrobacter sp. SC65A.3.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>SC65A.3 is a polyextremophile, growing up to 15 \u00b0C and tolerating 1.9 M NaCl and 0.9 M MgCl\u2082. Phylogenetic analysis classified it within P. cryohalolentis. Functional assays showed broad hydrolytic activity and resistance to 10 antibiotics across 8 classes, including third-generation cephalosporins, fluoroquinolones, aminoglycosides, and rifampicin. Whole-genome analysis identified &amp;gt;100 AMR-associated genes, including clinically relevant determinants (e.g., ampC, gyrA, gyrB, parC, parE, dfrA, rpoB, tetA, tetC, and mcr-1), as well as multiple heavy-metal resistance and multidrug efflux genes. SC65A.3 inhibited 14 ESKAPE-group pathogens (including MRSA, Enterococcus faecium, Enterobacter sp., Pseudomonas aeruginosa, Klebsiella pneumoniae, and Acinetobacter baumannii), consistent with genes linked to antimicrobial compounds such as glycopeptides and bacitracin. In addition, 45 stress-response genes related to cold\/heat adaptation were detected, including distinctive htpX, htpG, and pka genes among cold-adapted Psychrobacter.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>SC65A.3 represents an ancient, ice-adapted Psychrobacter with a dual profile of multidrug resistance and antimicrobial activity, highlighting ice caves as underexplored reservoirs of ancient resistomes and bioactive traits. To our knowledge, this is the first genome analysis of a Psychrobacter isolate from an ice cave and the first characterization of an ancient resistome from this environment, supporting future ecological, biotechnological, and medical exploration.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2025.1713017","type":"journal-article","created":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T06:33:32Z","timestamp":1771310012000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["First genome sequence and functional profiling of Psychrobacter SC65A.3 preserved in 5,000-year-old cave ice: insights into ancient resistome, antimicrobial potential, and enzymatic activities"],"prefix":"10.3389","volume":"16","author":[{"given":"Victoria Ioana","family":"Paun","sequence":"first","affiliation":[{"name":"Department of Microbiology, Institute of Biology Bucharest of the Romanian Academy","place":["Bucharest, Romania"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corina","family":"Itcus","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Institute of Biology Bucharest of the Romanian Academy","place":["Bucharest, Romania"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paris","family":"Lavin","sequence":"additional","affiliation":[{"name":"Departamento de Biotecnolog\u0131a, Facultad de Ciencias del Mar y Recursos Biologicos, Universidad de Antofagasta","place":["Antofagasta, Chile"]},{"name":"Centro de Investigaci\u00f3n en Inmunolog\u00eda y Biotecnolog\u00eda Biom\u00e9dica de Antofagasta (CIIBBA), Universidad de Antofagasta","place":["Antofagasta, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Carmen","family":"Chifiriuc","sequence":"additional","affiliation":[{"name":"Faculty of Biology and the Research Institute of the University of Bucharest, ICUB, University of Bucharest","place":["Bucharest, Romania"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Purcarea","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Institute of Biology Bucharest of the Romanian Academy","place":["Bucharest, Romania"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,2,17]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1016\/j.ejar.2016.04.003","article-title":"Characterization and bioremediation potential of marine Psychrobacter species","volume":"42","author":"Abd-Elnaby","year":"2016","journal-title":"Egypt. J. Aquat. Res."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"1791","DOI":"10.3390\/antibiotics11121791","article-title":"Combined anti-bacterial actions of Lincomycin and freshly prepared silver nanoparticles: overcoming the resistance to antibiotics and enhancement of the bioactivity","volume":"11","author":"Abdul-Jabbar","year":"2022","journal-title":"Antibiotics"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"fiaa260","DOI":"10.1093\/femsec\/fiaa260","article-title":"Are permafrost microorganisms as old as permafrost?","volume":"97","author":"Abramov","year":"2021","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"896","DOI":"10.1080\/1040841X.2024.2313024","article-title":"Antibiotic resistance: a global crisis, problems and solutions","volume":"50","author":"Aggarwal","year":"2024","journal-title":"Crit. Rev. Microbiol."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1007\/s10123-020-00153-x","article-title":"Bacterial community characterization of Batura glacier in the Karakoram range of Pakistan","volume":"24","author":"Ali","year":"2021","journal-title":"Int. Microbiol."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1038\/nrmicro2312","article-title":"Call of the wild: antibiotic resistance genes in natural environments","volume":"8","author":"Allen","year":"2010","journal-title":"Nat. Rev. Microbiol."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"e143","DOI":"10.1371\/journal.pcbi.0020143","article-title":"The evolution of two-component Systems in Bacteria Reveals Different Strategies for niche adaptation","volume":"2","author":"Alm","year":"2006","journal-title":"PLoS Comput. Biol."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. Mol. Biol."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"2748","DOI":"10.1093\/jac\/dkab212","article-title":"dfrA trimethoprim resistance genes found in Gram-negative bacteria: compilation and unambiguous numbering","volume":"76","author":"Ambrose","year":"2021","journal-title":"J. Antimicrob. Chemother."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"295","DOI":"10.5038\/1827-806X.48.3.2272","article-title":"Screening the cultivable cave microbial mats for the production of antimicrobial compounds and antibiotic resistance","volume":"48","author":"Ambrozic Avgustin","year":"2019","journal-title":"IJS"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"121035","DOI":"10.1016\/j.envpol.2023.121035","article-title":"Evaluating the impact of heavy metals on antimicrobial resistance in the primary food production environment: A scoping review","volume":"320","author":"Anedda","year":"2023","journal-title":"Environ. Pollut."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1016\/j.tree.2011.09.012","article-title":"Glaciers and ice sheets as a biome","volume":"27","author":"Anesio","year":"2012","journal-title":"Trends Ecol. Evol."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1038\/s41522-017-0019-0","article-title":"The microbiome of glaciers and ice sheets","volume":"3","author":"Anesio","year":"2017","journal-title":"NPJ Biofilms Microb."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"566","DOI":"10.1038\/nbt.4163","article-title":"KBase: the United States Department of Energy Systems Biology Knowledgebase","volume":"36","author":"Arkin","year":"2018","journal-title":"Nat. Biotechnol."},{"key":"ref15","doi-asserted-by":"publisher","first-page":"1645","DOI":"10.2147\/IDR.S173867","article-title":"Antibiotic resistance: a rundown of a global crisis","volume":"11","author":"Aslam","year":"2018","journal-title":"IDR Volume"},{"key":"ref16","doi-asserted-by":"publisher","first-page":"2304","DOI":"10.1128\/AEM.02101-09","article-title":"The genome sequence of Psychrobacter arcticus 273-4, a psychroactive Siberian permafrost bacterium, reveals mechanisms for adaptation to low-temperature growth","volume":"76","author":"Ayala-del-Rio","year":"2010","journal-title":"Appl. Environ. Microbiol."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"102","DOI":"10.3389\/fmicb.2011.00106","article-title":"Diversity and vertical distribution of microbial eukaryotes in the snow, sea ice and seawater near the north pole at the end of the polar night","volume":"2","author":"Bachy","year":"2011","journal-title":"Front. Microbiol."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"1631","DOI":"10.1099\/ijs.0.016782-0","article-title":"Psychrobacter aestuarii sp. nov., isolated from a tidal flat sediment","volume":"60","author":"Baik","year":"2010","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/femsec\/fiy102","article-title":"Adaptations to marine versus terrestrial low temperature environments as revealed by comparative genomic analyses of the genus Psychrobacter","volume":"94","author":"Bakermans","year":"2018","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"1285","DOI":"10.1099\/ijs.0.64043-0","article-title":"Psychrobacter cryohalolentis sp. nov. and Psychrobacter arcticus sp. nov., isolated from Siberian permafrost","volume":"56","author":"Bakermans","year":"2006","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1046\/j.1462-2920.2003.00419.x","article-title":"Reproduction and metabolism at \u2212 10\u00b0C of bacteria isolated from Siberian permafrost","volume":"5","author":"Bakermans","year":"2003","journal-title":"Environ. Microbiol."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1093\/ajcp\/45.4_ts.493","article-title":"Antibiotic susceptibility testing by a standardized single disk method","volume":"45","author":"Bauer","year":"1966","journal-title":"Am. J. Clin. Pathol."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"67","DOI":"10.3390\/geosciences10020067","article-title":"Bacterial communities of Novaya Zemlya archipelago ice and permafrost","volume":"10","author":"Belov","year":"2020","journal-title":"Geosciences"},{"key":"ref24","doi-asserted-by":"publisher","first-page":"108089","DOI":"10.1016\/j.envint.2023.108089","article-title":"Towards monitoring of antimicrobial resistance in the environment: for what reasons, how to implement it, and what are the data needs?","volume":"178","author":"Bengtsson-Palme","year":"2023","journal-title":"Environ. Int."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"28852","DOI":"10.1074\/jbc.M413750200","article-title":"BcrC from Bacillus subtilis acts as an Undecaprenyl pyrophosphate phosphatase in bacitracin resistance","volume":"280","author":"Bernard","year":"2005","journal-title":"J. Biol. Chem."},{"key":"ref26","doi-asserted-by":"publisher","first-page":"e34953","DOI":"10.1371\/journal.pone.0034953","article-title":"Antibiotic resistance is prevalent in an isolated cave microbiome","volume":"7","author":"Bhullar","year":"2012","journal-title":"PLoS One"},{"key":"ref27","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1111\/j.1365-2672.2007.03716.x","article-title":"Cyanobacteria from benthic mats of Antarctic lakes as a source of new bioactivities","volume":"105","author":"Biondi","year":"2008","journal-title":"J. Appl. Microbiol."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1038\/nrmicro3380","article-title":"Molecular mechanisms of antibiotic resistance","volume":"13","author":"Blair","year":"2015","journal-title":"Nat. Rev. Microbiol."},{"key":"ref29","doi-asserted-by":"publisher","first-page":"962452","DOI":"10.3389\/fmicb.2023.962452","article-title":"Diversity, distribution and organic substrates preferences of microbial communities of a low anthropic activity cave in North-Western Romania","volume":"14","author":"Bogdan","year":"2023","journal-title":"Front. Microbiol."},{"key":"ref30","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1186\/s12864-017-3543-7","article-title":"The nucleotide composition of microbial genomes indicates differential patterns of selection on core and accessory genomes","volume":"18","author":"Bohlin","year":"2017","journal-title":"BMC Genomics"},{"key":"ref31","doi-asserted-by":"publisher","first-page":"1942","DOI":"10.3201\/eid2410.171821","article-title":"Psychrobacter sanguinis<\/i> wound infection associated with marine environment exposure, Washington, USA","volume":"24","author":"Bonwitt","year":"2018","journal-title":"Emerg. Infect. Dis."},{"key":"ref32","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1099\/00207713-17-4-343","article-title":"A revised description of Moraxella polymorpha Flamm 1957, with a proposal of a new name, Moraxella phenylpyrouvica for this species","volume":"17","author":"Bovre","year":"1967","journal-title":"Int. J. Syst. Bacteriol."},{"key":"ref33","doi-asserted-by":"crossref","DOI":"10.1007\/0-387-30746-X_35","article-title":"The genus Psychrobacter","volume-title":"The Prokaryotes","author":"Bowman","year":"2006"},{"key":"ref34","doi-asserted-by":"publisher","first-page":"841","DOI":"10.1099\/00207713-46-4-841","article-title":"Novel Psychrobacter species from Antarctic ornithogenic soils","volume":"46","author":"Bowman","year":"1996","journal-title":"Int. J. Syst. Bacteriol."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1016\/S0723-2020(97)80067-7","article-title":"Psychrobacter glacincola sp. nov., a halotolerant, psychrophilic bacterium isolated from Antarctic Sea ice","volume":"20","author":"Bowman","year":"1997","journal-title":"Syst. Appl. Microbiol."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"1093","DOI":"10.1099\/ijs.0.02457-0","article-title":"Characterization of several Psychrobacter strains isolated from Antarctic environments and description of Psychrobacter luti sp. nov. and Psychrobacter fozii sp. nov","volume":"53","author":"Bozal","year":"2003","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"10096","DOI":"10.1038\/s41598-018-28401-1","article-title":"Fungi in perennial ice from Sc\u0103ri\u0219oara Ice Cave (Romania)","volume":"8","author":"Brad","year":"2018","journal-title":"Sci. Rep."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"573","DOI":"10.1128\/MMBR.64.3.573-606.2000","article-title":"Search and discovery strategies for biotechnology: the paradigm shift","volume":"64","author":"Bull","year":"2000","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"e01076","DOI":"10.1128\/AAC.01076-18","article-title":"Past and present perspectives on \u03b2-lactamases","volume":"62","author":"Bush","year":"2018","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"969","DOI":"10.1128\/AAC.01009-09","article-title":"Updated functional classification of \u03b2-lactamases","volume":"54","author":"Bush","year":"2010","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"1118","DOI":"10.3201\/eid1907.121599","article-title":"Psychrobacter arenosus bacteremia after blood transfusion, France","volume":"19","author":"Caspar","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"ref42","doi-asserted-by":"publisher","first-page":"1925","DOI":"10.1093\/bioinformatics\/btz848","article-title":"GTDB-Tk: a toolkit to classify genomes with the genome taxonomy database","volume":"36","author":"Chaumeil","year":"2020","journal-title":"Bioinformatics"},{"key":"ref43","doi-asserted-by":"publisher","first-page":"e00725","DOI":"10.1128\/genomeA.00725-13","article-title":"Complete genome sequence of Antarctic bacterium <i>Psychrobacter<\/i> sp. strain G","volume":"1","author":"Che","year":"2013","journal-title":"Genome Announc."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"1265","DOI":"10.5194\/tc-16-1265-2022","article-title":"Temporal variation of bacterial community and nutrients in Tibetan glacier snowpack","volume":"16","author":"Chen","year":"2022","journal-title":"Cryosphere"},{"key":"ref45","doi-asserted-by":"publisher","first-page":"14901","DOI":"10.1073\/pnas.221582098","article-title":"Glycopeptide antibiotic biosynthesis: enzymatic assembly of the dedicated amino acid monomer (S)-3,5-dihydroxyphenylglycine","volume":"98","author":"Chen","year":"2001","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref46","doi-asserted-by":"publisher","first-page":"e03845","DOI":"10.1128\/spectrum.03845-23","article-title":"Detection of resistance to macrolides and fluoroquinolones in Mycoplasma genitalium by targeted next-generation sequencing","volume":"12","author":"Chiribau","year":"2024","journal-title":"Microbiol. Spectr."},{"key":"ref47","doi-asserted-by":"publisher","first-page":"5242","DOI":"10.1128\/jb.175.16.5242-5252.1993","article-title":"Characterization of twenty-six new heat shock genes of Escherichia coli","volume":"175","author":"Chuang","year":"1993","journal-title":"J. Bacteriol."},{"key":"ref48","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1099\/00221287-42-3-345","article-title":"The role of penicillin Acylase in the resistance of Gram-negative Bacteria to Penicillins","volume":"42","author":"Cole","year":"1966","journal-title":"J. Gen. Microbiol."},{"key":"ref49","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1038\/nature10388","article-title":"Antibiotic resistance is ancient","volume":"477","author":"D\u2019Costa","year":"2011","journal-title":"Nature"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1002\/9781444317145.ch7","article-title":"Sea ice Bacteria and viruses","volume-title":"Sea Ice","author":"Deming","year":"2009"},{"key":"ref51","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1078\/0723-2020-00006","article-title":"Psychrobacter proteolyticus sp. nov., a Psychrotrophic, halotolerant bacterium isolated from the Antarctic krill Euphausia superba Dana, excreting a cold-adapted metalloprotease","volume":"24","author":"Denner","year":"2001","journal-title":"Syst. Appl. Microbiol."},{"key":"ref52","doi-asserted-by":"publisher","first-page":"W465","DOI":"10.1093\/nar\/gkn180","article-title":"Phylogeny.fr: robust phylogenetic analysis for the non-specialist","volume":"36","author":"Dereeper","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"ref53","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1099\/ijs.0.032631-0","article-title":"Psychrobacter isolates of human origin, other than Psychrobacter phenylpyruvicus, are predominantly Psychrobacter faecalis and Psychrobacter pulmonis, with emended description of P. faecalis","volume":"62","author":"Deschaght","year":"2012","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref54","doi-asserted-by":"publisher","first-page":"433","DOI":"10.1007\/s00792-013-0521-0","article-title":"Plasmid diversity in arctic strains of Psychrobacter spp","volume":"17","author":"Dziewit","year":"2013","journal-title":"Extremophiles"},{"key":"ref55","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1186\/s12917-022-03542-8","article-title":"Detection and description of a novel Psychrobacter glacincola infection in some Red Sea marine fishes in Hurghada, Egypt","volume":"19","author":"El-Sayed","year":"2023","journal-title":"BMC Vet. Res."},{"key":"ref56","doi-asserted-by":"publisher","first-page":"402","DOI":"10.3390\/ph18030402","article-title":"Combating antibiotic resistance: mechanisms, multidrug-resistant pathogens, and novel therapeutic approaches: An updated review","volume":"18","author":"Elshobary","year":"2025","journal-title":"Pharmaceuticals"},{"key":"ref57","doi-asserted-by":"publisher","first-page":"836","DOI":"10.1128\/CMR.14.4.836-871.2001","article-title":"Molecular detection of antimicrobial resistance","volume":"14","author":"Fluit","year":"2001","journal-title":"Clin. Microbiol. Rev."},{"key":"ref58","doi-asserted-by":"publisher","first-page":"e00486","DOI":"10.1128\/mSphere.00486-18","article-title":"Discovery of mcr-1 -mediated colistin resistance in a highly virulent Escherichia coli lineage","volume":"3","author":"Forde","year":"2018","journal-title":"mSphere"},{"key":"ref59","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1016\/j.enzmictec.2004.04.021","article-title":"A novel penicillin acylase from the environmental gene pool with improved synthetic properties","volume":"36","author":"Gabor","year":"2005","journal-title":"Enzym. Microb. Technol."},{"key":"ref60","doi-asserted-by":"publisher","first-page":"105773","DOI":"10.1016\/j.ibiod.2024.105773","article-title":"From cultural and natural heritage to reservoir of biomedicine: prospection of bioactive compounds produced by bacterial isolates from caves","volume":"190","author":"Gatinho","year":"2024","journal-title":"Int. Biodeterior. Biodegrad."},{"key":"ref61","doi-asserted-by":"publisher","first-page":"1212378","DOI":"10.3389\/fmicb.2023.1212378","article-title":"A comparative approach to confirm antibiotic-resistant microbes in the cryosphere","volume":"14","author":"Gattinger","year":"2023","journal-title":"Front. Microbiol."},{"key":"ref62","doi-asserted-by":"publisher","first-page":"001333","DOI":"10.1099\/mic.0.001333","article-title":"Role of bacterial efflux pumps in antibiotic resistance, virulence, and strategies to discover novel efflux pump inhibitors","volume":"169","author":"Gaurav","year":"2023","journal-title":"Microbiology"},{"key":"ref63","doi-asserted-by":"publisher","first-page":"1351","DOI":"10.1007\/s00300-010-0824-x","article-title":"Production of antibiotics and enzymes by soil microorganisms from the windmill islands region, Wilkes Land, East Antarctica","volume":"33","author":"Gesheva","year":"2010","journal-title":"Polar Biol."},{"key":"ref64","doi-asserted-by":"publisher","first-page":"1390","DOI":"10.3390\/catal11111390","article-title":"Cold-active lipase-based biocatalysts for silymarin valorization through biocatalytic acylation of silybin","volume":"11","author":"Gheorghita","year":"2021","journal-title":"Catalysts"},{"key":"ref65","doi-asserted-by":"publisher","first-page":"fuae017","DOI":"10.1093\/femsre\/fuae017","article-title":"Unravelling the mechanisms of antibiotic and heavy metal resistance co-selection in environmental bacteria","volume":"48","author":"Gillieatt","year":"2024","journal-title":"FEMS Microbiol. Rev."},{"key":"ref66","doi-asserted-by":"publisher","first-page":"400","DOI":"10.1128\/jcm.28.2.400-401.1990","article-title":"Ocular infection caused by Psychrobacter immobilis acquired in the hospital","volume":"28","author":"Gini","year":"1990","journal-title":"J. Clin. Microbiol."},{"key":"ref67","doi-asserted-by":"publisher","first-page":"4948","DOI":"10.1038\/s41467-020-18770-5","article-title":"Antibiotic export by MexB multidrug efflux transporter is allosterically controlled by a MexA-OprM chaperone-like complex","volume":"11","author":"Glavier","year":"2020","journal-title":"Nat. Commun."},{"key":"ref68","doi-asserted-by":"publisher","first-page":"625","DOI":"10.1038\/ja.2014.107","article-title":"Resistance to rifampicin: a review","volume":"67","author":"Goldstein","year":"2014","journal-title":"J. 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Methods"},{"key":"ref72","doi-asserted-by":"publisher","first-page":"100126","DOI":"10.1016\/j.crmicr.2022.100126","article-title":"Environmental factors affecting the diversity of psychrophilic microbial community in the high altitude snow-fed lake Hemkund, India","volume":"3","author":"Gupta","year":"2022","journal-title":"Curr. Res. Microb. Sci."},{"key":"ref73","doi-asserted-by":"publisher","first-page":"D851","DOI":"10.1093\/nar\/gkx1068","article-title":"RefSeq: an update on prokaryotic genome annotation and curation","volume":"46","author":"Haft","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref74","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1128\/jcm.19.2.279-280.1984","article-title":"Five-hour novobiocin test for differentiation of coagulase-negative staphylococci","volume":"19","author":"Harrington","year":"1984","journal-title":"J. Clin. 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J. Syst. Evol. Microbiol."},{"key":"ref241","doi-asserted-by":"publisher","first-page":"1885","DOI":"10.1099\/ijs.0.63682-0","article-title":"Psychrobacter celer sp. nov., isolated from sea water of the South Sea in Korea","volume":"55","author":"Yoon","year":"","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref242","doi-asserted-by":"publisher","first-page":"1007","DOI":"10.1099\/ijs.0.63464-0","article-title":"Psychrobacter aquimaris sp. nov. and Psychrobacter namhaensis sp. nov., isolated from sea water of the South Sea in Korea","volume":"55","author":"Yoon","year":"","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref243","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1099\/ijs.0.63140-0","article-title":"Psychrobacter alimentarius sp. nov., isolated from squid jeotgal, a traditional Korean fermented seafood","volume":"55","author":"Yoon","year":"","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref244","doi-asserted-by":"publisher","first-page":"1281","DOI":"10.3390\/md12031281","article-title":"Phylogenetic diversity and biological activity of Actinobacteria isolated from the Chukchi shelf marine sediments in the Arctic Ocean","volume":"12","author":"Yuan","year":"2014","journal-title":"Mar. Drugs"},{"key":"ref245","doi-asserted-by":"publisher","first-page":"1985","DOI":"10.1099\/ijs.0.02686-0","article-title":"Psychrobacter okhotskensis sp. nov., a lipase-producing facultative psychrophile isolated from the coast of the Okhotsk Sea","volume":"53","author":"Yumoto","year":"2003","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref246","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1099\/ijs.0.010959-0","article-title":"Psychrobacter piscatorii sp. nov., a psychrotolerant bacterium exhibiting high catalase activity isolated from an oxidative environment","volume":"60","author":"Yumoto","year":"2010","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref247","doi-asserted-by":"publisher","first-page":"5091","DOI":"10.1099\/ijsem.0.001476","article-title":"Psychrobacter pocilloporae sp. nov., isolated from a coral, Pocillopora eydouxi","volume":"66","author":"Zachariah","year":"2016","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref248","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1007\/s00248-021-01889-3","article-title":"Cave microbes as a potential source of drugs development in the modern era","volume":"84","author":"Zada","year":"2022","journal-title":"Microb. Ecol."},{"key":"ref249","doi-asserted-by":"publisher","first-page":"20004","DOI":"10.1073\/pnas.0907925106","article-title":"Overexpression of the chromosomally encoded aminoglycoside acetyltransferase eis confers kanamycin resistance in Mycobacterium tuberculosis","volume":"106","author":"Zaunbrecher","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref250","doi-asserted-by":"publisher","first-page":"1283","DOI":"10.1007\/s10482-015-0580-6","article-title":"Psychrobacter fjordensis sp. nov., a psychrotolerant bacterium isolated from an Arctic fjord in Svalbard","volume":"108","author":"Zeng","year":"2015","journal-title":"Antonie Van Leeuwenhoek"},{"key":"ref251","doi-asserted-by":"publisher","first-page":"1792","DOI":"10.1099\/ijsem.0.000939","article-title":"Psychrobacter glaciei sp. nov., isolated from the ice core of an Arctic glacier","volume":"66","author":"Zeng","year":"2016","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref252","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1078\/1438-4221-00195","article-title":"Molecular analysis of efflux pump-based antibiotic resistance","volume":"292","author":"Zgurskaya","year":"2002","journal-title":"Int. J. Med. 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