{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T20:46:23Z","timestamp":1790196383665,"version":"4.1.0"},"reference-count":60,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T00:00:00Z","timestamp":1764288000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      The growing prevalence of multidrug-resistant\n                      <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                      and nontuberculous mycobacteria (NTM) highlights the urgent need for alternative therapeutic approaches. Mycobacteriophages, viruses that selectively infect mycobacteria, have emerged as promising tools. Here, we report the isolation and characterization of a new subcluster K4 phage, Yasnaya_Polyana, with a focus on its regulatory motifs and engineered lytic variant.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The phage was isolated by enrichment on\n                      <jats:italic>Mycobacterium smegmatis<\/jats:italic>\n                      mc(2)155, followed by genome sequencing and functional annotation. Start-Associated Sequences (SAS) and Extended SAS (ESAS) were analyzed\n                      <jats:italic>in silico<\/jats:italic>\n                      across 188 cluster K phages. A lytic derivative, YP\u039447, was engineered by deleting the repressor gene and characterized in terms of morphology, stability, infection dynamics, and host range.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Yasnaya_Polyana exhibited siphovirus morphology and high genetic similarity with other subcluster K4 phages. Regulatory motif analysis revealed a reduced abundance of SAS and ESAS elements in subcluster K4 phages, including Yasnaya_Polyana, along with specific ESAS sequence deviations. The engineered YP\u039447 mutant retained morphology and infection parameters comparable to the wild-type phage but exhibited a decline in lysogeny frequency (from 18% to &amp;lt;0.01%), confirming a lytic phenotype. Host range analysis revealed limited activity of YP\u039447 against NTM, while the phage demonstrated a high efficiency of plating (EOP = 10\n                      <jats:sup>\u22121<\/jats:sup>\n                      ) on\n                      <jats:italic>M. tuberculosis<\/jats:italic>\n                      H37Rv and effectively lysed clinical isolates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These findings suggest that Yasnaya Polyana, and apparently other subcluster K4 phages, harbor distinct regulatory features that may reflect divergent transcriptional control strategies. Moreover, YP\u039447 shows potential as a candidate for phage therapy targeting mycobacterial infections.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2025.1713073","type":"journal-article","created":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T09:58:37Z","timestamp":1764323917000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Mycobacteriophage Yasnaya_Polyana and its engineered lytic derivative: specificity of regulatory motifs and lytic potential"],"prefix":"10.3389","volume":"16","author":[{"given":"Egor","family":"Shitikov","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maja","family":"Malakhova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofya","family":"Kuznetsova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitry","family":"Bespiatykh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roman","family":"Gorodnichev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergei","family":"Kiselev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Kornienko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ksenia","family":"Klimina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandra","family":"Strokach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arina","family":"German","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasiia","family":"Lebedeva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikhail","family":"Fursov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Vnukova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitry","family":"Bagrov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia","family":"Kazyulina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margarita","family":"Shleeva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Zaychikova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,11,28]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1214","DOI":"10.3390\/microorganisms11051214","article-title":"Experimental studies of the liposomal form of lytic mycobacteriophage D29 for the Treatment of tuberculosis infection","volume":"11","author":"Avdeev","year":"2023","journal-title":"Microorganisms"},{"key":"B2","doi-asserted-by":"publisher","first-page":"10961","DOI":"10.1073\/pnas.94.20.10961","article-title":"Conditionally replicating mycobacteriophages: a system for transposon delivery to Mycobacterium tuberculosis","volume":"94","author":"Bardarov","year":"1997","journal-title":"Proc. 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