{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T16:57:06Z","timestamp":1787936226357,"version":"build-2784847793"},"reference-count":62,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T00:00:00Z","timestamp":1766102400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Phagocytosis is a pivotal component of the human innate immune defense against malaria. This essential defense mechanism is often modulated by various host-derived soluble factors. We investigated the phagocytosis of\n                      <jats:italic>Plasmodium falciparum<\/jats:italic>\n                      - and\n                      <jats:italic>P. knowlesi<\/jats:italic>\n                      -infected erythrocytes (IRBC) by human monocytic THP-1 cells in the presence of periostin (OSF-2), a human secretory protein involved in inflammation and tissue repair. This focus was prompted by the fact that OSF-2 is a potent stimulator of\n                      <jats:italic>Plasmodium<\/jats:italic>\n                      IRBC rosette formation, a parasite-derived cytoadherence phenomenon known to impede phagocytosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Culturable parasite isolates were recruited, and tested with the THP-1 cells and recombinant human OSF-2 protein. The role of OSF-2 in IRBC phagocytosis by the phagocytes was evaluated in the presence and absence of uninfected erythrocytes (URBC), and the receptor involved was investigated with antibody blocking assay.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>OSF-2 exerted a dual role. When rosetting was prevented via IRBC purification, OSF-2 increased IRBC phagocytosis. This stimulatory effect was also seen when THP-1 cells were primed with OSF-2 before IRBC exposure. This OSF-2-mediated phagocytosis was CD36-dependent and rapidly reversible upon OSF-2 removal. However, when rosetting was induced by the addition of URBC, the presence of OSF-2 reduced the rate of IRBC phagocytosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These findings highlight the complex parasite-host interactions influencing the infection pathogenesis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2025.1728562","type":"journal-article","created":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T06:25:38Z","timestamp":1766125538000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of periostin (OSF-2) on phagocytosis of Plasmodium-infected erythrocytes"],"prefix":"10.3389","volume":"16","author":[{"given":"Joo-Yie","family":"Chin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammed-Nur-Iman","family":"Mohammed-Syafiei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Jun","family":"Lim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon Xue-Zhen","family":"Chong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad-Nasreen","family":"Suhaimi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Ying","family":"Phong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yee Ling","family":"Ng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yee-Ling","family":"Lau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I-Ching","family":"Sam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"R\u00e9nia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenn-Chyau","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,12,19]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"S340","DOI":"10.1086\/374747","article-title":"Phagocytosis and the inflammatory response","volume":"187","author":"Aderem","year":"2003","journal-title":"J. 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