{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T12:25:34Z","timestamp":1778502334953,"version":"3.51.4"},"reference-count":43,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T00:00:00Z","timestamp":1768953600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Diabetes mellitus poses a global health burden with limited therapies derived from breast milk targeting its systemic complications. Probiotics like\n                      <jats:italic>Lactobacillus helveticus<\/jats:italic>\n                      show metabolic benefits, but strain-specific efficacy and mechanisms remain unclear.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      This study evaluated\n                      <jats:italic>L. helveticus<\/jats:italic>\n                      WIS02\u2019s therapeutic potential in streptozotocin (STZ)-induced diabetic mice, focusing on glycemic control, tissue protection, and gut microbiota modulation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We found pre-administration of WIS02 for 7\u202fdays reduced the final fasting blood glucose by 66.0% and improved oral glucose tolerance (AUC decreased by 51.6%). Additionally, WIS02 significantly mitigated dyslipidemia (TC decreased by 23.6%, TG decreased by 39.5%) and alleviated tissue damage to the liver, pancreatic, and colon tissues. Microbiota analysis revealed that WIS02 treatment significantly altered the gut microbiota structure, increasing the proportion of beneficial bacteria, particularly\n                      <jats:italic>Akkermansia muciniphila<\/jats:italic>\n                      and\n                      <jats:italic>Parabacteroides distasonis.<\/jats:italic>\n                      Correlation analysis between microbiota abundance and physiological indicators showed that\n                      <jats:italic>Parabacteroides distasonis<\/jats:italic>\n                      ,\n                      <jats:italic>Oscillibacter valericigenes<\/jats:italic>\n                      , and\n                      <jats:italic>Akkermansia muciniphila<\/jats:italic>\n                      were significantly negatively correlated with blood glucose and lipid levels, while positively correlated with insulin and hepatic glycogen levels.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      <jats:italic>L. helveticus<\/jats:italic>\n                      WIS02 exhibits multi-targeted efficacy against diabetes by regulating glycolipid metabolism, protecting tissues, and remodeling gut microbiota. These findings highlight its potential as a novel probiotic for metabolic disorder management, warranting clinical translation.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2025.1735605","type":"journal-article","created":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T06:41:23Z","timestamp":1768977683000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Novel probiotic Lactobacillus helveticus WIS02 alleviates diabetes through multi-pronged regulation of glycolipid metabolism, pancreatic protection and gut microbiota remodeling"],"prefix":"10.3389","volume":"16","author":[{"given":"Shuang","family":"Guo","sequence":"first","affiliation":[{"name":"School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine","place":["Tianjin, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuying","family":"Li","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine","place":["Tianjin, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanan","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine","place":["Tianjin, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuexiao","family":"Jiang","sequence":"additional","affiliation":[{"name":"PKUMed-Wisbiom Joint Laboratory for Human Microbiome Research","place":["Beijing, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yufeng","family":"Wang","sequence":"additional","affiliation":[{"name":"PKUMed-Wisbiom Joint Laboratory for Human Microbiome Research","place":["Beijing, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Cao","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine","place":["Tianjin, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunfeng","family":"Duan","sequence":"additional","affiliation":[{"name":"PKUMed-Wisbiom Joint Laboratory for Human Microbiome Research","place":["Beijing, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chongming","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine","place":["Tianjin, China"]},{"name":"State Key Laboratory of Chinese Medicine Modernization","place":["Tianjin, China"]},{"name":"Tianjin Key Laboratory of Therapeutic Substance of Traditional Chinese Medicine","place":["Tianjin, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,1,21]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"167483","DOI":"10.1016\/j.bbadis.2024.167483","article-title":"Probiotic therapy modulates the brain-gut-liver microbiota axis in a mouse model of traumatic brain injury","volume":"1870","author":"Amaral","year":"2024","journal-title":"Biochim. 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