{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T19:06:31Z","timestamp":1785870391971,"version":"3.56.0"},"reference-count":33,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T00:00:00Z","timestamp":1769040000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      JR20, a novel sesamin-derived arylnaphthalene lignan, has demonstrated potent antifungal activity. This study further investigates its antibacterial potential against MRSA (methicillin-resistant\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                      ).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The highlights of this research include the use of SYTO9 and PI fluorescence double staining, along with three-dimensional confocal microscopy to reveal the thickness and viability of biofilms under JR0\u2032s influence. Additionally, scanning and transmission electron microscopy were employed to observe the morphological changes of MRSA under JR0\u2032s impact. By combining the observed reduction in ATP content within MRSA, a preliminary mechanism was hypothesized.\n                      <jats:italic>In vivo<\/jats:italic>\n                      anti-infection experiments were further conducted to evaluate the compound's biological activity in liver and spleen tissues of mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      JR20 exhibited potent anti-MRSA activity (IC\n                      <jats:sub>50<\/jats:sub>\n                      = 20.88 \u03bcg\/mL). Mechanistic investigations revealed multi-level effects: confocal microscopy demonstrated altered biofilm thickness and viability; SEM\/TEM confirmed distinct morphological changes in bacterial cells; And ATP content reduction indicated metabolic disruption.\n                      <jats:italic>In vivo<\/jats:italic>\n                      experiments validated these antibacterial effects and further revealed anti-inflammatory properties, underscoring JR0\u2032s therapeutic potential against MRSA infections.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study confirms JR0\u2032s potent anti-MRSA activity, clarifies its effects on biofilms and MRSA morphology, and proposes a preliminary mechanism by reduced ATP. JR20 demonstrates significant potential for combating drug-resistant bacteria and advancing antibiofilm drug discovery.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2025.1743534","type":"journal-article","created":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T06:53:26Z","timestamp":1769064806000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["JR20, a novel natural product-derived compound, exhibits potent anti-biofilm activity against methicillin-resistant Staphylococcus aureus"],"prefix":"10.3389","volume":"16","author":[{"given":"Meirong","family":"Zhao","sequence":"first","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Guangxi Minzu University","place":["Nanning, China"]},{"name":"College of Food and Pharmaceutical Engineering, Guangxi Vocational University of Agriculture","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaowei","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Shenzhen Technology University","place":["Shenzhen, China"]},{"name":"School of Chinese Medicine, Hong Kong Baptist University","place":["Kowloon Tong, Hong Kong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yogini","family":"Jaiswal","sequence":"additional","affiliation":[{"name":"Center for Excellence in Post-Harvest Technologies, North Carolina Agricultural and Technical State University, The North Carolina Research Campus","place":["Kannapolis, NC, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinrong","family":"Xie","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Guangxi Minzu University","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongyu","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Shenzhen University Medical School, Shenzhen University","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhendan","family":"He","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Shenzhen Technology University","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonard","family":"Williams","sequence":"additional","affiliation":[{"name":"Center for Excellence in Post-Harvest Technologies, North Carolina Agricultural and Technical State University, The North Carolina Research Campus","place":["Kannapolis, NC, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifu","family":"Guan","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Guangxi Minzu University","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hedong","family":"Bian","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Guangxi Minzu University","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xun","family":"Song","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Shenzhen Technology University","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,1,22]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"484","DOI":"10.1038\/s41586-025-09278-3","article-title":"Neutrophils drive vascular occlusion, tumour necrosis and metastasis","volume":"645","author":"Adrover","year":"2025","journal-title":"Nature"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1493","DOI":"10.3389\/fmicb.2018.01493","article-title":"Synergistic anti-MRSA activity of cationic nanostructured lipid carriers in combination with oxacillin for cutaneous application","volume":"9","author":"Alalaiwe","year":"2018","journal-title":"Front. 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