{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:19:41Z","timestamp":1783383581049,"version":"3.54.6"},"reference-count":60,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T00:00:00Z","timestamp":1767744000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    This study examined the influence of tannic acid (TA) on the fermentation rate of inulin (INU), fructooligosaccharides (FOS), galacto-oligosaccharides, xylo-oligosaccharides, isomalto-oligosaccharides, and\n                    <jats:italic>\u03b2<\/jats:italic>\n                    -glucan. It was found that 1.5\u202f\u03bcmol\/mL TA decreased production of gas and short-chain fatty acids from fermentation of these fibers, confirming that TA decreased the fermentation rate. Moreover, 1.5\u202f\u03bcmol\/mL TA inhibited the activity of\n                    <jats:italic>\u03b2<\/jats:italic>\n                    -fructosidase, xylanase,\n                    <jats:italic>\u03b2<\/jats:italic>\n                    -galactosidase,\n                    <jats:italic>\u03b1<\/jats:italic>\n                    -glucosidase, and\n                    <jats:italic>\u03b2<\/jats:italic>\n                    -glucanase secreted by gut bacteria and decreased the fermentation rate of glucose, fructose, xylose, and galactose. In summary, TA inhibited the activity of enzymes that participated in metabolism of dietary fibers and thus decreased the fermentation rate. On the other hand, INU and FOS were selected as representatives of dietary fibers, and the effect of interplay between TA and INU or FOS on the composition of gut microbiota was analyzed. TA and FOS or INU selectively promoted the growth of different specific genera, which might also result in the decrease in the fermentation rate. Moreover, co-fermentation of TA and FOS or INU increased the abundance of beneficial bacteria, including\n                    <jats:italic>Faecalibacterium<\/jats:italic>\n                    and\n                    <jats:italic>Coprococcus<\/jats:italic>\n                    , at the late fermentation stage, which suggest that decreasing the fermentation rate of fibers was beneficial for the distal colon health.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2025.1751028","type":"journal-article","created":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T07:00:30Z","timestamp":1767769230000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Tannic acid delayed dietary fiber fermentation: dual mechanisms of enzyme inhibition and microbial community dynamics"],"prefix":"10.3389","volume":"16","author":[{"given":"Liping","family":"Ren","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoqiang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunjing","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengmei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuting","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,1,7]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"2829","DOI":"10.1080\/10408398.2024.2352523","article-title":"Sustainable diets with plant-based proteins require considerations for prevention of proteolytic fermentation","volume":"65","author":"Boven","year":"2025","journal-title":"Crit. 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Colon Rectum"},{"key":"ref10","doi-asserted-by":"publisher","first-page":"1404","DOI":"10.1053\/j.gastro.2023.08.052","article-title":"Traditional medicine Pien Tze Huang suppresses colorectal tumorigenesis through restoring gut microbiota and metabolites","volume":"165","author":"Gou","year":"2023","journal-title":"Gastroenterology"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"110758","DOI":"10.1016\/j.foodhyd.2024.110758","article-title":"Exploring the role of polysaccharides from macroalgae in shaping gut microbiome and promoting anti-obesity outcomes","volume":"160","author":"Goushki","year":"2025","journal-title":"Food Hydrocoll."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1016\/j.tifs.2019.09.005","article-title":"Dietary polyphenols to combat the metabolic diseases via altering gut microbiota","volume":"93","author":"Gowd","year":"2019","journal-title":"Trends Food Sci. 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Food Chem."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"573","DOI":"10.1038\/nrgastro.2017.88","article-title":"Gut microbiota and IBD: causation or correlation?","volume":"14","author":"Ni","year":"2017","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"Article 1897212","DOI":"10.1080\/19490976.2021.1897212","article-title":"The role of short-chain fatty acids in the interplay between gut microbiota and diet in cardio-metabolic health","volume":"13","author":"Nogal","year":"2021","journal-title":"Gut Microbes"},{"key":"ref40","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1017\/s0007114500000325","article-title":"In vitro fermentability of dextran, oligodextran and maltodextrin by human gut bacteria","volume":"83","author":"Olano-Martin","year":"2000","journal-title":"Br. J. Nutr."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1078\/0723-2020-00101","article-title":"Purification and characterisation of an extracellular fructan \u03b2-fructosidase from a Lactobacillus pentosus strain isolated from fermented fish","volume":"25","author":"Paludan-M\u00fcller","year":"2002","journal-title":"Syst. Appl. Microbiol."},{"key":"ref42","doi-asserted-by":"publisher","first-page":"216","DOI":"10.1016\/j.foodchem.2017.04.115","article-title":"Binding selectivity of dietary polyphenols to different plant cell wall components: quantification and mechanism","volume":"233","author":"Phan","year":"2017","journal-title":"Food Chem."},{"key":"ref43","doi-asserted-by":"publisher","first-page":"02032","DOI":"10.3389\/fmicb.2020.02032","article-title":"Berry polyphenols and fibers modulate distinct microbial metabolic functions and gut microbiota enterotype-like clustering in obese mice","volume":"11","author":"Rodr\u00edguez-Daza","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"1464","DOI":"10.1128\/jcm.02427-10","article-title":"Cultivable anaerobic microbiota of severe early childhood caries","volume":"49","author":"Tanner","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"ref45","doi-asserted-by":"publisher","first-page":"116271","DOI":"10.1016\/j.foodres.2025.116271","article-title":"Specific dietary fibers steer toward distal colonic saccharolytic fermentation using the microbiota of individuals with overweight\/obesity","volume":"209","author":"van Deuren","year":"2025","journal-title":"Food Res. Int."},{"key":"ref46","doi-asserted-by":"publisher","first-page":"3692","DOI":"10.1128\/aem.71.7.3692-3700.2005","article-title":"pH and peptide supply can radically alter bacterial populations and short-chain fatty acid ratios within microbial communities from the human colon","volume":"71","author":"Walker","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"key":"ref47","doi-asserted-by":"publisher","first-page":"12323","DOI":"10.1021\/acs.jafc.1c04039.s001","article-title":"Starch microspheres entrapped with chitosan delay in vitro fecal fermentation and regulate human gut microbiota composition","volume":"69","author":"Wang","year":"2021","journal-title":"J. Agric. Food Chem."},{"key":"ref48","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.tifs.2019.03.005","article-title":"In vitro colonic fermentation of dietary fibers: fermentation rate, short-chain fatty acid production and changes in microbiota","volume":"88","author":"Wang","year":"2019","journal-title":"Trends Food Sci. Technol."},{"key":"ref49","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1038\/s41571-023-00766-x","article-title":"Gut microbiota in colorectal cancer development and therapy","volume":"20","author":"Wong","year":"2023","journal-title":"Nat. Rev. Clin. Oncol."},{"key":"ref50","doi-asserted-by":"publisher","first-page":"141010","DOI":"10.2139\/ssrn.4846746","article-title":"RG-I pectic polysaccharides and hesperidin synergistically modulate gut microbiota: an in vitro study targeting the proportional relationship","volume":"462","author":"Wu","year":"2025","journal-title":"Food Chem."},{"key":"ref51","doi-asserted-by":"publisher","first-page":"104171","DOI":"10.1016\/j.tifs.2023.104171","article-title":"Natural polyphenols-gut microbiota interactions and effects on glycolipid metabolism via polyphenols-gut-brain axis: a state-of-the-art review","volume":"140","author":"Xie","year":"2023","journal-title":"Trends Food Sci. 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