{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T06:11:41Z","timestamp":1778739101344,"version":"3.51.4"},"reference-count":16,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T00:00:00Z","timestamp":1778716800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>The fluorescence quantitative PCR method for the detection of Vero cell residual DNA in human rabies vaccine urgently needs a national standard for Vero cell DNA quantification.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Briefly, the genomic DNA of Vero cells was extracted, diluted to 100 \u03bcg\/ml, and divided into 0.1-ml aliquots in sterile screw cap tubes. After DNA preparation, concentration calibration, applicability, and stability verification were carried out by multiple laboratories.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The concentration of the national standard for Vero cell DNA quantification (limited qPCR method) determined from this collaborative study was 107 \u00b1 6 \u03bcg\/mL, and the DNA purity (OD\n                      <jats:sub>260<\/jats:sub>\n                      \/OD\n                      <jats:sub>280<\/jats:sub>\n                      ) was 1.83. This standard is only suitable for the qPCR method, which has an average amplification efficiency of 92%. After accelerated degradation at 37 \u00b0C from 1 to 4 weeks, the standard showed amplification curves similar to those stored at \u221270 \u00b0C, with no significant difference in amplification efficiency, showing good stability.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study successfully developed a national standard for Vero cell DNA quantification using the quantitative fluorescence PCR method, which has significant application potential for standardizing Vero cell DNA residue detection in human rabies vaccines.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1708044","type":"journal-article","created":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T05:33:10Z","timestamp":1778736790000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Development and validation of a Vero cell DNA standard for residual DNA measurement suitable for quantitative PCR method"],"prefix":"10.3389","volume":"17","author":[{"given":"Danhua","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Arbovirus Vaccines, National Institutes for Food and Drug Control","place":["Beijing, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunpeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Arbovirus Vaccines, National Institutes for Food and Drug Control","place":["Beijing, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohong","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Arbovirus Vaccines, National Institutes for Food and Drug Control","place":["Beijing, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leitai","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Arbovirus Vaccines, National Institutes for Food and Drug Control","place":["Beijing, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Arbovirus Vaccines, National Institutes for Food and Drug Control","place":["Beijing, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuhua","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Arbovirus Vaccines, National Institutes for Food and Drug Control","place":["Beijing, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shouchun","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Arbovirus Vaccines, National Institutes for Food and Drug Control","place":["Beijing, China"]},{"name":"Research Units of Innovative Vaccine Quality Evaluation and Standardization, Chinese Academy of Medical Sciences","place":["Beijing, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,5,14]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1016\/j.biologicals.2016.03.002","article-title":"Universal realtime PCR assay for quantitation and size evaluation of residual cell DNA in human viral vaccines","volume":"44","author":"Andr\u00e9","year":"2016","journal-title":"Biologicals"},{"key":"B2","doi-asserted-by":"publisher","first-page":"596","DOI":"10.1631\/jzus.B1000171","article-title":"Cloning of long tandem repeat sequence of Vero cells and its application in quantitative PCR for DNA","volume":"24","author":"Cao","year":"2011","journal-title":"Chin. 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