{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T15:25:25Z","timestamp":1782487525731,"version":"3.54.5"},"reference-count":39,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T00:00:00Z","timestamp":1774224000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>This study investigated the effects of a selective soil treatment agent on the microbial community structure of tobacco rhizosphere soil and the photosynthetic performance of tobacco plants at different temperatures (15\u202f\u00b0C, 25\u202f\u00b0C, 35\u202f\u00b0C) through pot experiments.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The control efficacy against black shank and root rot diseases was also evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results and discussion<\/jats:title>\n                    <jats:p>\n                      The results indicated that 25\u202f\u00b0C was the optimal temperature for the agent\u2019s effectiveness. Under this condition, the agent significantly inhibited the enrichment of phytopathogenic fungi such as\n                      <jats:italic>Fusarium<\/jats:italic>\n                      spp. in the rhizosphere soil (the abundance of\n                      <jats:italic>Fusarium<\/jats:italic>\n                      in the T25SJ treatment decreased by 78.6% compared to the untreated group T25CK), while optimizing the microbial community structure, as evidenced by increased fungal\n                      <jats:italic>\u03b1<\/jats:italic>\n                      -diversity (Shannon index) and enhanced competitiveness of beneficial bacteria (e.g.,\n                      <jats:italic>Pseudomonas<\/jats:italic>\n                      spp.). Notably, the bacterial community stability was maintained (no significant change in the Simpson index), confirming the agent\u2019s selective regulatory effect rather than non-specific sterilization. Fluorescence imaging analysis revealed that the photosynthetic performance of tobacco plants treated with the agent at 25\u202f\u00b0C was significantly higher than that of the untreated group, as showed by an increase in the maximum photochemical efficiency (Fv\/Fm) (T25SJ vs. T25CK: 0.82 vs. 0.68), enhanced electron transport efficiency (\u03c8_Eo), and light energy conversion capacity (\u03b4_Ro). In contrast, the untreated group exhibited severe damage to the photosynthetic system due to pathogen infection, leading to the inactivation of PSII reaction centers (increased ABS\/RC and decreased RC\/ABS values). At low (15\u202f\u00b0C) and high (35\u202f\u00b0C) temperatures, the agent still showed some inhibitory effects on certain pathogens (e.g.,\n                      <jats:italic>Thielaviopsis<\/jats:italic>\n                      spp.), but the overall antibacterial efficiency and photosynthetic protection were significantly weaker than at 25\u202f\u00b0C. Further analysis demonstrated that the agent alleviated oxidative stress caused by root damage and ensured the preferential allocation of light energy to photosynthesis by selectively suppressing dominant pathogens while preserving beneficial microbial populations. This study reveals that the antibacterial efficacy and photosynthetic protection of the soil treatment agent are significantly temperature-dependent, with 25\u202f\u00b0C being the optimal application temperature. These findings provide a theoretical basis for the green control of soil-borne diseases in tobacco and the regulation of photosynthetic stress resistance, and offer a new paradigm for resolving the contradiction between \u201cpathogen control\u201d and \u201cecological protection\u201d in soil-borne disease management.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1717189","type":"journal-article","created":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T06:43:17Z","timestamp":1774248197000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Study on a temperature-dependent selective soil treatment agent in regulating tobacco rhizosphere microbial community and controlling root and stem diseases"],"prefix":"10.3389","volume":"17","author":[{"given":"Yuanhua","family":"Wu","sequence":"first","affiliation":[{"name":"Tobacco Research Institute of Chinese Academy of Agricultural Sciences","place":["Qingdao, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Fang","sequence":"additional","affiliation":[{"name":"Hunan Tobacco Company Chenzhou City Branch","place":["Chenzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaye","family":"Chen","sequence":"additional","affiliation":[{"name":"Hunan Tobacco Company Chenzhou City Branch","place":["Chenzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linghua","family":"Liu","sequence":"additional","affiliation":[{"name":"Hunan Tobacco Company Chenzhou City Branch","place":["Chenzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxin","family":"Wu","sequence":"additional","affiliation":[{"name":"Hunan Tobacco Company Chenzhou City Branch","place":["Chenzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Liu","sequence":"additional","affiliation":[{"name":"Hunan Tobacco Company Chenzhou City Branch","place":["Chenzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Liu","sequence":"additional","affiliation":[{"name":"Hunan Tobacco Company Chenzhou City Branch","place":["Chenzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Xing","sequence":"additional","affiliation":[{"name":"Energy-rich Compounds Production by Photosynthetic Carbon Fixation Research Center, Shandong Provincial Key Laboratory of Microbial Resource Exploration and Innovative Utilization, College of Life Sciences, Qingdao Agricultural University","place":["Qingdao, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weitao","family":"Wang","sequence":"additional","affiliation":[{"name":"China Tobacco Shandong Industrial Co., Ltd., Qingdao Cigarette Factory","place":["Qingdao, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangliang","family":"Liu","sequence":"additional","affiliation":[{"name":"Tobacco Research Institute of Chinese Academy of Agricultural Sciences","place":["Qingdao, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aiguo","family":"Chen","sequence":"additional","affiliation":[{"name":"Tobacco Research Institute of Chinese Academy of Agricultural Sciences","place":["Qingdao, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaobao","family":"Wang","sequence":"additional","affiliation":[{"name":"Energy-rich Compounds Production by Photosynthetic Carbon Fixation Research Center, Shandong Provincial Key Laboratory of Microbial Resource Exploration and Innovative Utilization, College of Life Sciences, Qingdao Agricultural University","place":["Qingdao, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,3,23]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"100014","DOI":"10.1016\/j.ncrops.2024.100014","article-title":"Advances in understanding the plant-Ralstonia solanacearum interactions: unraveling the dynamics, mechanisms, and implications for crop disease resistance","volume":"1","author":"An","year":"2024","journal-title":"New Crops"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"971","DOI":"10.1094\/pdis-06-23-1138-re","article-title":"Behavior and use of quaternary ammonium-based disinfectants in biosafety protocols against Fusarium oxysporum f. sp. cubense race 1 and tropical race 4","volume":"108","author":"Arango-Palacio","year":"2024","journal-title":"Plant Dis."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"84","DOI":"10.36959\/771\/565","article-title":"Biology and integrated control of tomato wilt caused by Fusarium oxysporum lycopersici: a comprehensive review under the light of recent advancements","volume":"3","author":"Aref","year":"2020","journal-title":"Scholars. Direct"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"3766","DOI":"10.1016\/j.csbj.2020.10.045","article-title":"Phytophthora zoospores: from perception of environmental signals to inoculum formation on the host-root surface","volume":"18","author":"Bassani","year":"2020","journal-title":"Comput. Struct. Biotechnol. J."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"e70027","DOI":"10.1111\/gcbb.70027","article-title":"Utilization of municipal biowaste-derived compounds to reduce soilborne fungal diseases of tomato: a further step toward circular bioeconomy","volume":"17","author":"Castello","year":"2025","journal-title":"GCB Bioenergy"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"101355","DOI":"10.1016\/j.jafr.2024.101355","article-title":"Enhancing the effects of solarization-based approaches to suppress Verticillium dahliae inocula affecting tomato in greenhouse","volume":"18","author":"Castello","year":"2024","journal-title":"J. Agric. Food Res."},{"key":"ref7","first-page":"585404","article-title":"Microbial Interactions within Multiple-Strain Biological Control Agents Impact Soil-Borne Plant Disease","volume-title":"Front. Microbiol.","author":"Chen","year":"2020"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"4059","DOI":"10.3390\/plants12234059","article-title":"Exploring the differential impact of salt stress on root colonization adaptation mechanisms in plant growth-promoting rhizobacteria","volume":"12","author":"del Lorena Rosario","year":"2023","journal-title":"Plants"},{"key":"ref10","doi-asserted-by":"publisher","first-page":"e005","DOI":"10.1002\/mbo3.70005","article-title":"Monitoring the persistence of Pseudomonas sivasensis strain CF10PS3 in cereal fields","volume":"13","author":"Delitte","year":"2024","journal-title":"Microbiol. Open"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"105610","DOI":"10.1016\/j.cropro.2021.105610","article-title":"LAMP detection of the blackleg pathogen Leptosphaeria biglobosa \u2018canadensis\u2019","volume":"145","author":"Du","year":"2021","journal-title":"Crop Prot."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"113","DOI":"10.26737\/ij-mds.v7i2.5203","article-title":"Farmer behaviour of certified high-yielding seed users increases tobacco productivity","volume":"7","author":"Dudung Ahmad","year":"2024","journal-title":"Int. J. Multi Discipline Sci."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1111\/j.1364-3703.2007.00465.x","article-title":"Phytophthora infestans: the plant (and R gene) destroyer","volume":"9","author":"Fry","year":"2008","journal-title":"Mol. Plant Pathol."},{"key":"ref14","first-page":"2251","article-title":"Effects of different fumigation on continuous cropping soil in peach orchard","volume":"34","author":"Gao","year":"2022","journal-title":"Acta Agric. Zhejiangensis"},{"key":"ref15","volume-title":"Fusarium","author":"Garc\u00eda-Enciso","year":"2017"},{"key":"ref16","first-page":"76","article-title":"Epidemiology and control of tobacco black shank","volume":"9","author":"Ge","year":"1996","journal-title":"Southwest China J. Agric. Sci."},{"key":"ref17","first-page":"510","volume-title":"Tobacco Control Policy Analysis in China: Economics and Health","author":"Hu","year":"2009"},{"key":"ref18","doi-asserted-by":"publisher","first-page":"109456","DOI":"10.1016\/j.scienta.2020.109456","article-title":"Impacts of sequential herbicides and residue mulching on weeds and productivity and profitability of vegetable pea in north-western indo-Gangetic Plains","volume":"270","author":"Kaur","year":"2020","journal-title":"Sci. Hortic."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"932","DOI":"10.1890\/08-0079.1","article-title":"The ecology of climate change and infectious diseases","volume":"90","author":"Lafferty","year":"2009","journal-title":"Ecology"},{"key":"ref20","doi-asserted-by":"publisher","first-page":"157834","DOI":"10.1016\/j.scitotenv.2022.157834","article-title":"The impact of heat and inflow wind variations on vertical transport around a supertall building \u2013 the one Vanderbilt field experiment","volume":"851","author":"Lamer","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"1022","DOI":"10.1094\/PDIS.1998.82.9.1022","article-title":"Efficacy of various fungal and bacterial biocontrol organisms for control of Fusarium wilt of tomato","volume":"82","author":"Larkin","year":"1998","journal-title":"Plant Dis."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"1414","DOI":"10.3389\/fpls.2019.01414","article-title":"Effects of high temperature and drought stress on the expression of gene encoding enzymes and the activity of key enzymes involved in starch biosynthesis in wheat grains","volume":"10","author":"Li","year":"2019","journal-title":"Front. Plant Sci."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"12581","DOI":"10.1038\/s41598-017-12985-1","article-title":"Temporal patterns of cotton Fusarium and Verticillium wilt in Jiangsu coastal areas of China","volume":"7","author":"Li","year":"2017","journal-title":"Sci. Rep."},{"key":"ref24","doi-asserted-by":"publisher","first-page":"1489993","DOI":"10.3389\/fpls.2024.1489993","article-title":"Tobacco production under global climate change: combined effects of heat and drought stress and coping strategies","volume":"15","author":"Liu","year":"2024","journal-title":"Front. Plant Sci."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"5531","DOI":"10.1128\/aem.00925-07","article-title":"Quantification of 2,4-diacetylphloroglucinol-producing Pseudomonas fluorescens strains in the plant rhizosphere by real-time PCR","volume":"73","author":"Mavrodi","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1186\/s12870-025-06080-1","article-title":"Foliar application of 24-epibrassinolide enhances leaf nicotine content under low temperature conditions during the mature stage of flue-cured tobacco by regulating cold stress tolerance","volume":"25","author":"Niu","year":"2025","journal-title":"BMC Plant Biol."},{"key":"ref26","doi-asserted-by":"publisher","first-page":"16","DOI":"10.3390\/agriculture10010016","article-title":"Methods for management of soilborne diseases in crop production","volume":"10","author":"Panth","year":"2020","journal-title":"Agriculture"},{"key":"ref27","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/j.cropro.2017.08.006","article-title":"Effectiveness of seven commercial rootstocks against verticillium wilt and their effects on growth, yield, and fruit quality of tomato","volume":"102","author":"Papadaki","year":"2017","journal-title":"Crop Prot."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1007\/s42161-024-01604-4","article-title":"Soil amendments for management of Phytophthora root rot in avocado and their impact on the soil microbiome","volume":"106","author":"Qurrat Ul Ain","year":"2024","journal-title":"J. Plant Pathol."},{"key":"ref29","doi-asserted-by":"publisher","first-page":"662","DOI":"10.1002\/cpt.2333","article-title":"PharmVar GeneFocus: CYP2C9","volume":"110","author":"Sangkuhl","year":"2021","journal-title":"Clin. Pharmacol. Therap."},{"key":"ref30","doi-asserted-by":"publisher","first-page":"1109154","DOI":"10.3389\/fpls.2023.1109154","article-title":"Temperature increase modifies susceptibility to Verticillium wilt in Medicago spp and may contribute to the emergence of more aggressive pathogenic strains","volume":"14","author":"Sbeiti","year":"2023","journal-title":"Front. Plant Sci."},{"key":"ref31","volume-title":"Probing Photosynthesis: Mechanisms, Regulation & Adaptation","author":"Strasser","year":"2000"},{"key":"ref32","doi-asserted-by":"publisher","first-page":"R619","DOI":"10.1016\/j.cub.2018.03.054","article-title":"Plant-pathogen warfare under changing climate conditions","volume":"28","author":"Vel\u00e1squez","year":"2018","journal-title":"Curr. Biol."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"e0260524","DOI":"10.1128\/spectrum.02605-24","article-title":"Microbial fertilizers modulate tobacco growth and development through reshaping soil microbiome and metabolome","volume":"13","author":"Wang","year":"2025","journal-title":"Microbiol. Spectrum"},{"key":"ref34","doi-asserted-by":"publisher","first-page":"903555","DOI":"10.3389\/fbioe.2022.903555","article-title":"Ralstonia solanacearum infection disturbed the microbiome structure throughout the whole tobacco crop niche as well as the nitrogen metabolism in soil","volume":"10","author":"Wang","year":"2022","journal-title":"Front. Bioeng. Biotechnol."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"6970","DOI":"10.1038\/s41467-025-62326-4","article-title":"A fungal effector promotes infection via stabilizing a negative regulatory factor of chloroplast immunity","volume":"16","author":"Xiao","year":"2025","journal-title":"Nat. Commun."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"1496385","DOI":"10.3389\/fmicb.2025.1496385","article-title":"Effects of long-term continuous cropping on microbial community structure and function in tobacco rhizosphere soil","volume":"16","author":"Yang","year":"2025","journal-title":"Front. Microbiol."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1186\/s41938-024-00831-2","article-title":"Microbial community composition and their activity against Phytophthora nicotianae at different growth stages of tobacco","volume":"34","author":"Zhang","year":"2024","journal-title":"Egypt. J. Biol. Pest Control"},{"key":"ref38","first-page":"179","article-title":"Deciphering Pathogenicity of Fusarium oxysporum From a Phylogenomics Perspective","volume-title":"Advances in Genetics","author":"Zhang","year":"2017"},{"key":"ref39","doi-asserted-by":"publisher","first-page":"24","DOI":"10.3390\/ijms24108881","article-title":"Novel 1,3,4-Thiadiazole derivatives: synthesis, antiviral bioassay and regulation the photosynthetic pathway of tobacco against TMV infection","volume":"24","author":"Zheng","year":"2023","journal-title":"Int. J. Mol. 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