{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T12:08:47Z","timestamp":1777637327723,"version":"3.51.4"},"reference-count":42,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Traditional Chinese medicine (TCM) offers multi-targeted therapy with few side effects for ulcerative colitis (UC). Taoling Yuyang Decoction (TLYY) has shown clinical efficacy, however, its mechanisms of action are unclear. This study employed a multi-omics approach to elucidate TLYY\u2019s therapeutic effects in a murine DSS-induced colitis model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Using a DSS-induced mouse model of colitis, animals were divided into five groups (\n                      <jats:italic>n<\/jats:italic>\n                      = 8): blank control (CK), model (DSS), low- and high-dose TLYY (TLYYL, TLYYH), and positive control (5-ASA). Treatment efficacy was assessed by measuring body weight, disease activity index (DAI), and histopathological changes. Oxidative stress markers (MDA, GSH-Px, SOD) in serum and colon tissues were quantified using commercial kits and immunohistochemistry. Cytokine levels (IL-1\u03b2, TNF-\u03b1, IL-6) and LPS were measured by ELISA. The expression of tight junction proteins (ZO-1, Occludin, Claudin-1) in colon tissue was analyzed via qPCR and immunocytochemistry. Additionally, 16S rDNA sequencing and metabolomics were employed to examine gut microbiota composition, fecal SCFAs, and differential metabolites.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Taoling Yuyang Decoction treatment dose-dependently alleviated UC symptoms, including weight loss, colon shortening, and tissue damage. It enhanced antioxidant activities (SOD, GSH-Px) and reduced MDA, suppressed pro-inflammatory cytokines and LPS, and up-regulated tight junction proteins (ZO-1, Occludin, Claudin-1). Gut microbiota analysis showed TLYY-H restored microbial diversity, increased beneficial bacteria (e.g.,\n                      <jats:italic>Lactobacillus<\/jats:italic>\n                      ,\n                      <jats:italic>Akkermansia<\/jats:italic>\n                      ), and decreased harmful genera (e.g.,\n                      <jats:italic>Helicobacter<\/jats:italic>\n                      ). It also elevated SCFA levels and modulated fecal metabolism, up-regulating anti-inflammatory\/antioxidant metabolites while down-regulating toxic compounds. Key pathways such as AGE-RAGE signaling and xenobiotic metabolism were restored.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Taoling Yuyang Decoction ameliorates DSS-induced colitis via integrated mechanisms involving oxidative stress reduction, anti-inflammation, intestinal barrier repair, and restoration of gut flora homeostasis and metabolic pathways. The superior effect of the high dose highlights its clinical potential for UC treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1750380","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:07:12Z","timestamp":1777442832000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Taoling Yuyang Decoction alleviates DSS-induced ulcerative colitis in mice by regulating gut microbiota -metabolite axis and restore intestinal barrier function"],"prefix":"10.3389","volume":"17","author":[{"given":"Dongyang","family":"Liu","sequence":"first","affiliation":[{"name":"The First Department of Gastroenterology, The First Affiliated Hospital of Heilongjiang University of Chinese Medicine","place":["Harbin, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deshuang","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, Qiqihar Medical University","place":["Qiqihar, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ziwen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, Qiqihar Medical University","place":["Qiqihar, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, Qiqihar Medical University","place":["Qiqihar, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuang","family":"Liu","sequence":"additional","affiliation":[{"name":"The First Department of Gastroenterology, The First Affiliated Hospital of Heilongjiang University of Chinese Medicine","place":["Harbin, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinlai","family":"Song","sequence":"additional","affiliation":[{"name":"College of Basic Medicine, Heilongjiang University of Chinese Medicine","place":["Harbin, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xingrui","family":"Cui","sequence":"additional","affiliation":[{"name":"Jiangsu Hospital with Integration of Chinese and Western Medicine, Nanjing University of Chinese Medicine","place":["Nanjing, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuoyue","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, Qiqihar Medical University","place":["Qiqihar, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, Qiqihar Medical University","place":["Qiqihar, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoying","family":"Liang","sequence":"additional","affiliation":[{"name":"The First Department of Gastroenterology, The First Affiliated Hospital of Heilongjiang University of Chinese Medicine","place":["Harbin, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunjing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, Qiqihar Medical University","place":["Qiqihar, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,4,29]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1016\/j.jare.2023.08.008","article-title":"Targeting Desulfovibrio vulgaris flagellin-induced NAIP\/NLRC4 inflammasome activation in macrophages attenuates ulcerative colitis.","volume":"52","author":"An","year":"2023","journal-title":"J. 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