{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T05:28:16Z","timestamp":1792301296354,"version":"4.4.0"},"reference-count":26,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Background and objective<\/jats:title>\n                    <jats:p>Brucellosis is a common zoonotic disease worldwide. Because its symptoms are non-specific and laboratory findings vary, diagnosis remains challenging. Blood culture is still the gold standard for confirmation, but its yield is often limited. This study aimed to estimate blood-culture positivity among hospitalized male patients with newly diagnosed brucellosis and to identify clinical and laboratory factors independently associated with culture positivity within confirmed cases.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We conducted a hospital-based retrospective study of 1,188 hospitalized men with newly diagnosed brucellosis admitted to a tertiary infectious-disease hospital in China between 2021 and 2023. Demographics, clinical manifestations, laboratory parameters, and blood-culture results were collected. Multivariate logistic regression was used to identify independent predictors of a positive blood culture.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The overall blood-culture positivity rate was 30.9%. Univariate analysis indicated that disease stage, fatigue, fever, anorexia, splenomegaly, arthritis, paraspinal abscess, joint effusion, platelet count (PLT), alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT), albumin (ALB), total bilirubin, lactate dehydrogenase (LDH), serum agglutination test (SAT), procalcitonin (PCT), and erythrocyte sedimentation rate (ESR) were associated with culture positivity. After multivariate adjustment, LDH, SAT, PCT, and ESR emerged as independent predictors of a positive blood culture, with PCT showing the strongest predictive value. Higher levels of LDH, SAT, PCT and ESR were independently associated with higher positive rates of blood cultures.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      In this hospital-based retrospective cohort of confirmed brucellosis, LDH, SAT titer, PCT, and ESR were independently associated with blood-culture positivity. These routinely available parameters may help risk-stratify bacteremic likelihood and support culture-related decision-making (e.g., prioritizing sampling and considering repeat cultures when clinically warranted) among SAT-positive (or otherwise confirmed) patients, but they should not be interpreted as standalone markers for diagnosing\n                      <jats:italic>Brucella<\/jats:italic>\n                      bacteremia without microbiological confirmation.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1754087","type":"journal-article","created":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T06:47:21Z","timestamp":1770187641000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Blood culture positivity and its clinical determinants among hospitalized male patients with newly diagnosed, laboratory-confirmed brucellosis: a hospital-based retrospective study"],"prefix":"10.3389","volume":"17","author":[{"given":"Xue","family":"Han","sequence":"first","affiliation":[{"name":"Department of Infectious Disease, The Fourth People\u2019s Hospital of Ningxia Hui Autonomous Region","place":["Yinchuan, Ningxia, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiqing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease, The Fourth People\u2019s Hospital of Ningxia Hui Autonomous Region","place":["Yinchuan, Ningxia, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bianxia","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease, The Fourth People\u2019s Hospital of Ningxia Hui Autonomous Region","place":["Yinchuan, Ningxia, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weijie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease, The Fourth People\u2019s Hospital of Ningxia Hui Autonomous Region","place":["Yinchuan, Ningxia, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease, The Fourth People\u2019s Hospital of Ningxia Hui Autonomous Region","place":["Yinchuan, Ningxia, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beibei","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease, The Fourth People\u2019s Hospital of Ningxia Hui Autonomous Region","place":["Yinchuan, Ningxia, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanlong","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease, The Fourth People\u2019s Hospital of Ningxia Hui Autonomous Region","place":["Yinchuan, Ningxia, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease, The Fourth People\u2019s Hospital of Ningxia Hui Autonomous Region","place":["Yinchuan, Ningxia, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenhua","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease, The Fourth People\u2019s Hospital of Ningxia Hui Autonomous Region","place":["Yinchuan, Ningxia, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofang","family":"Shan","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease, The Fourth People\u2019s Hospital of Ningxia Hui Autonomous Region","place":["Yinchuan, Ningxia, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease, The Fourth People\u2019s Hospital of Ningxia Hui Autonomous Region","place":["Yinchuan, Ningxia, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,2,4]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1089\/vbz.2012.0997","article-title":"Factors affecting Brucella spp. blood cultures positivity in children","volume":"13","author":"Apa","year":"2013","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref2","volume-title":"Sentinel level clinical laboratory guidelines for suspected agents of bioterrorism and emerging infectious diseases. Guideline: brucellosis-causing Brucella species (BBS)","year":"2025"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"1110907","DOI":"10.3389\/fmed.2023.1110907","article-title":"A follow-up study of 100 patients with acute brucellosis for its prognosis and prevention","volume":"10","author":"Bai","year":"2023","journal-title":"Front. Med."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"e1865","DOI":"10.1371\/journal.pntd.0001865","article-title":"Global burden of human brucellosis: a systematic review of disease frequency","volume":"6","author":"Dean","year":"2012","journal-title":"PLoS Negl. Trop. 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Pak."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1080\/01652176.2020.1868616","article-title":"Bovine brucellosis - a comprehensive review","volume":"41","author":"Khurana","year":"2021","journal-title":"Vet. Q."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"e43758","DOI":"10.7759\/cureus.43758","article-title":"Comparison of the characteristics of Brucella patients diagnosed with blood culture positivity and\/or serology","volume":"15","author":"Konya","year":"2023","journal-title":"Cureus"},{"key":"ref14","doi-asserted-by":"publisher","first-page":"1789","DOI":"10.3201\/eid2909.230052","article-title":"Global estimate of human brucellosis incidence","volume":"29","author":"Laine","year":"2023","journal-title":"Emerg. Infect. Dis."},{"key":"ref15","doi-asserted-by":"publisher","first-page":"1565","DOI":"10.2147\/IDR.S202440","article-title":"Spinal brucellosis in Hulunbuir, China, 2011-2016","volume":"12","author":"Liang","year":"2019","journal-title":"Infect. Drug Resist."},{"key":"ref16","doi-asserted-by":"publisher","first-page":"1259479","DOI":"10.3389\/fmicb.2023.1259479","article-title":"Epidemiology, clinical manifestations, and laboratory findings of 1,590 human brucellosis cases in Ningxia, China","volume":"14","author":"Liu","year":"2023","journal-title":"Front. Microbiol."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"26345","DOI":"10.1038\/s41598-024-77391-w","article-title":"Epidemiology and laboratory testing of Brucella spondylitis","volume":"14","author":"Lu","year":"2024","journal-title":"Sci. Rep."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"fmab114","DOI":"10.1093\/tropej\/fmab114","article-title":"Bacteremic and Nonbacteremic brucellosis in children in Turkey","volume":"68","author":"\u00d6zdem","year":"2022","journal-title":"J. Trop. Pediatr."},{"key":"ref19","doi-asserted-by":"publisher","first-page":"2325","DOI":"10.1056\/NEJMra050570","article-title":"Brucellosis","volume":"352","author":"Pappas","year":"2005","journal-title":"N. Engl. J. Med."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"440","DOI":"10.1080\/00365540902968043","article-title":"Brucellar epididymo-orchitis: review of 53 cases in Babol, northern Iran","volume":"41","author":"Roushan","year":"2009","journal-title":"Scand. J. Infect. Dis."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"1954","DOI":"10.1016\/S0022-5347(01)63206-3","article-title":"Granulomatous epididymo-orchitis: sonographic features and clinical outcome in brucellosis, tuberculosis and idiopathic granulomatous epididymo-orchitis","volume":"159","author":"Salmeron","year":"1998","journal-title":"J. Urol."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"667","DOI":"10.1016\/j.tmaid.2014.10.005","article-title":"Brucellar epididymo-orchitis: a retrospective multicenter study of 28 cases and review of the literature","volume":"12","author":"Savasci","year":"2014","journal-title":"Travel Med. Infect. Dis."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"2741","DOI":"10.2147\/IDR.S319595","article-title":"Epidemiological, clinical and laboratory characteristics of patients with Brucella infection in Anhui Province, China","volume":"14","author":"Shi","year":"2021","journal-title":"Infect. Drug Resist."},{"key":"ref24","doi-asserted-by":"publisher","first-page":"34","DOI":"10.2174\/157489113805290746","article-title":"Clinical presentations and diagnosis of brucellosis","volume":"8","author":"Ulu-Kilic","year":"2013","journal-title":"Recent Pat. Antiinfect. Drug Discov."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"2161","DOI":"10.3760\/cma.j.issn.0376-2491.2016.27.010","article-title":"Multivariate analysis of blood culture positive rate of ICU patients","volume":"96","author":"Wu","year":"2016","journal-title":"Zhonghua Yi Xue Za Zhi"},{"key":"ref26","doi-asserted-by":"publisher","first-page":"e00073","DOI":"10.1128\/CMR.00073-19","article-title":"Laboratory diagnosis of human brucellosis","volume":"33","author":"Yagupsky","year":"2019","journal-title":"Clin. Microbiol. 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