{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T14:36:43Z","timestamp":1789569403658,"version":"build-2803163510"},"reference-count":49,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science & Technology Project of Wenzhou City, China","award":["N20210001"],"award-info":[{"award-number":["N20210001"]}]},{"name":"Medical Health Science and Technology Project of Zhejiang Provincial Health Commission","award":["2023KY1350"],"award-info":[{"award-number":["2023KY1350"]}]},{"name":"Zhejiang Provincial Natural Science Foundation of China","award":["LGF21H180012"],"award-info":[{"award-number":["LGF21H180012"]}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Bacterial resistance to aminoglycoside drugs is becoming increasingly severe due to their widespread use in agriculture and human medicine. Based on the whole-genome sequencing and molecular methods, a novel aminoglycoside resistance gene designated\n                    <jats:italic>aadA34<\/jats:italic>\n                    was identified from a multiple-drug-resistant bacterium\n                    <jats:italic>Serratia ureilytica<\/jats:italic>\n                    S24, which was isolated from sewage at a poultry farm. The\n                    <jats:italic>aadA34<\/jats:italic>\n                    gene conferred resistance to spectinomycin and streptomycin, with MIC levels increased by 64- and 128-fold, respectively, for spectinomycin and streptomycin compared with the control strain (pUCP20\/\n                    <jats:italic>E. coli<\/jats:italic>\n                    DH5\u03b1). The amino acid (aa) sequence of AadA34 was found to share the highest identity (60.09%) with the function-characterized aminoglycoside nucleotidyltransferase AadA16. Corresponding to the MIC results of the\n                    <jats:italic>aadA34<\/jats:italic>\n                    gene, the enzyme AadA34 exhibited high affinity and catalytic efficiency against spectinomycin or streptomycin. Approximately 160 aa longer than other functionally characterized AadA proteins, the\n                    <jats:italic>aadA34<\/jats:italic>\n                    gene was found to be generally encoded in bacteria of the genus\n                    <jats:italic>Serratia<\/jats:italic>\n                    , especially in the species\n                    <jats:italic>S. ureilytica<\/jats:italic>\n                    and\n                    <jats:italic>S. marcescens<\/jats:italic>\n                    , which were isolated from various sources including some of clinical significance.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1765554","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T06:59:11Z","timestamp":1773817151000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Characterization of a novel aminoglycoside resistance gene, aadA34, identified from Serratia ureilytica S24"],"prefix":"10.3389","volume":"17","author":[{"given":"Chaoqun","family":"Liu","sequence":"first","affiliation":[{"name":"Medical Molecular Biology Laboratory, School of Medicine, Jinhua University of Vocational Technology","place":["Jinhua, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhigang","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Translational Medicine, Baotou Central Hospital","place":["Baotou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shenghai","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Hangzhou First People\u2019s Hospital Affiliated of Westlake University School of Medicine","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiyao","family":"Cao","sequence":"additional","affiliation":[{"name":"Medical Molecular Biology Laboratory, School of Medicine, Jinhua University of Vocational Technology","place":["Jinhua, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Wenren","sequence":"additional","affiliation":[{"name":"Medical Molecular Biology Laboratory, School of Medicine, Jinhua University of Vocational Technology","place":["Jinhua, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanfang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Medical Molecular Biology Laboratory, School of Medicine, Jinhua University of Vocational Technology","place":["Jinhua, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiyue","family":"Feng","sequence":"additional","affiliation":[{"name":"Medical Molecular Biology Laboratory, School of Medicine, Jinhua University of Vocational Technology","place":["Jinhua, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junwan","family":"Lu","sequence":"additional","affiliation":[{"name":"Medical Molecular Biology Laboratory, School of Medicine, Jinhua University of Vocational Technology","place":["Jinhua, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojun","family":"Xu","sequence":"additional","affiliation":[{"name":"Zhejiang Pharmaceutical University","place":["Ningbo, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowei","family":"Chen","sequence":"additional","affiliation":[{"name":"Medical Molecular Biology Laboratory, School of Medicine, Jinhua University of Vocational Technology","place":["Jinhua, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiyu","family":"Bao","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Informatics\/School of Laboratory Medicine and Life Sciences, Wenzhou Medical University","place":["Wenzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teng","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Translational Medicine, Baotou Central Hospital","place":["Baotou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Hangzhou First People\u2019s Hospital Affiliated of Westlake University School of Medicine","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,3,18]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"22327","DOI":"10.1074\/jbc.M116.747865","article-title":"Pseudouridine in the anticodon of Escherichia coli tRNATyr(QPsiA) is catalyzed by the dual specificity enzyme RluF","volume":"291","author":"Addepalli","year":"2016","journal-title":"J. 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