{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T13:56:59Z","timestamp":1772632619451,"version":"3.50.1"},"reference-count":28,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T00:00:00Z","timestamp":1772582400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>Respiratory syncytial virus (RSV) presents a significant global public health challenge, contributing substantially to the disease burden worldwide. As numerous F protein-based vaccine candidates advance into clinical trials, robust evaluation methods are essential. Here, we developed a cell-based ELISA platform to rapidly evaluate serum antibody responses to these vaccines. By treating RSV-infected cells with 4% formaldehyde or 60% methanol, the cell-surface F proteins were stabilized in the pre-fusion (pre-F) or post-fusion (post-F) conformations, respectively. This platform efficiently determines the binding and neutralizing activities of post-immunization serum antibodies, providing an effective method for evaluating the efficacy of RSV vaccine candidates.<\/jats:p>","DOI":"10.3389\/fmicb.2026.1771336","type":"journal-article","created":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T07:02:48Z","timestamp":1772607768000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Optimally fixed F protein on the surface of RSV-infected cells for RSV binding and neutralizing assays"],"prefix":"10.3389","volume":"17","author":[{"given":"Hongsheng","family":"Qiang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, Department of Laboratory Medicine, School of Public Health, Xiamen University","place":["Xiamen, Fujian, China"]},{"name":"National Institute of Diagnostics and Vaccine Development in 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of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University","place":["Xiamen, Fujian, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, Department of Laboratory Medicine, School of Public Health, Xiamen University","place":["Xiamen, Fujian, China"]},{"name":"National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University","place":["Xiamen, Fujian, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongpeng","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, Department of Laboratory Medicine, School of Public Health, Xiamen University","place":["Xiamen, Fujian, 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Xiamen University, Xiamen University","place":["Xiamen, Fujian, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ningshao","family":"Xia","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, Department of Laboratory Medicine, School of Public Health, Xiamen University","place":["Xiamen, Fujian, China"]},{"name":"National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University","place":["Xiamen, Fujian, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,3,4]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"3254","DOI":"10.1038\/s41564-024-01860-1","article-title":"A foldon-free prefusion F trimer vaccine for respiratory syncytial virus to reduce off-target immune 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