{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T17:58:26Z","timestamp":1781632706023,"version":"3.54.5"},"reference-count":39,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T00:00:00Z","timestamp":1770681600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"\n Helicobacter pylori (H. pylori) is a key pathogen in gastritis and gastric ulcers, noted for its high recurrence rates.\n H. pylori<\/jats:italic>\n internalization into\n Candida<\/jats:italic>\n vacuoles may enhance its survival and pathogenicity. In this study, we investigated the intrinsic association between\n H. pylori<\/jats:italic>\n and\n Candida<\/jats:italic>\n by performing\n H. pylori<\/jats:italic>\n -related assays on\n Candida<\/jats:italic>\n isolates obtained from clinical fecal samples, gastric mucosal tissues, and vaginal secretions. About 399 isolates of\n Candida<\/jats:italic>\n were isolated from fecal samples of patients with digestive diseases.\n H. pylori<\/jats:italic>\n antigens in feces and 16S rDNA within\n Candida<\/jats:italic>\n isolates were detected, showing a significant correlation. After co-culturing\n H. pylori<\/jats:italic>\n and\n Candida in vitro<\/jats:italic>\n ,\n H. pylori<\/jats:italic>\n 16S rDNA-positive\n Candida<\/jats:italic>\n (CacoHp) were obtained.\n In vitro<\/jats:italic>\n , the CacoHp strain showed enhanced inhibitory and adhesive effects on GES-1 cells than standard\n Candida<\/jats:italic>\n strain.\n H. pylori<\/jats:italic>\n 16S rDNA was detected in CacoHp and\n Candida<\/jats:italic>\n isolates obtained from gastric mucosal tissue and vaginal secretions. CacoHp and\n H. pylori<\/jats:italic>\n 16S rDNA-positive clinical isolates exhibited urease activity, as evidenced by the color change of urea containing Sabouraud glucose agar medium from yellow to red. Gene expression analysis indicated that the\n H. pylori ureA<\/jats:italic>\n gene was expressed at low levels in the CacoHp and\n H. pylori<\/jats:italic>\n 16S rDNA-positive clinical isolates under the experimental conditions. Using direct immunofluorescence and fluorescence\n in situ<\/jats:italic>\n hybridization,\n H. pylori<\/jats:italic>\n antigens and genes were detected in\n Candida<\/jats:italic>\n . Fluorescent staining indicated the potential presence of viable bacteria within\n Candida<\/jats:italic>\n , suggesting that viable\n H. pylori<\/jats:italic>\n might be present intracellularly within these\n Candida<\/jats:italic>\n cells. These findings suggest that\n H. pylori<\/jats:italic>\n may persist within\n Candida<\/jats:italic>\n , with potential implications for human health.\n <\/jats:p>","DOI":"10.3389\/fmicb.2026.1774342","type":"journal-article","created":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T06:46:57Z","timestamp":1770706017000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Candida serves as a reservoir associating with facilitating the survival and pathogenesis of Helicobacter pylori"],"prefix":"10.3389","volume":"17","author":[{"given":"Jianchao","family":"Sun","sequence":"first","affiliation":[{"name":"Guizhou Key Laboratory of Microbio and Infectious Disease Prevention and Control, School of Basic Medical Science, Guizhou Medical University, Guiyang","place":["Guizhou, China"]},{"name":"Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education and Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang","place":["Guizhou, China"]},{"name":"Guizhou Provincial Center for Clinical Laboratory, Guiyang","place":["Guizhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingxiu","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Hospital Infection and Management, Guizhou Provincial People\u2019s Hospital, Guiyang","place":["Guizhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Luo","sequence":"additional","affiliation":[{"name":"The Affiliated Cancer Hospital of Guizhou Medical University, Guiyang","place":["Guizhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[{"name":"The Affiliated Cancer Hospital of Guizhou Medical University, Guiyang","place":["Guizhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Affiliated Cancer Hospital of Guizhou Medical University, Guiyang","place":["Guizhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenghong","family":"Chen","sequence":"additional","affiliation":[{"name":"Guizhou Key Laboratory of Microbio and Infectious Disease Prevention and Control, School of Basic Medical Science, Guizhou Medical University, Guiyang","place":["Guizhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,2,10]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"101297","DOI":"10.1016\/j.funeco.2023.101297","article-title":"Mr. Toad\u2019s wild fungi: Fungal isolate diversity on colorado boreal toads and their capacity for pathogen inhibition.","volume":"66","author":"Alexiev","year":"2023","journal-title":"Fungal Ecol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"306","DOI":"10.1053\/j.gastro.2007.11.009","article-title":"Host-bacterial interactions in Helicobacter pylori infection.","volume":"134","author":"Amieva","year":"2008","journal-title":"Gastroenterology"},{"key":"B3","doi-asserted-by":"publisher","first-page":"e1009515","DOI":"10.1371\/journal.ppat.1009515","article-title":"The cross-kingdom interaction between Helicobacter pylori and Candida albicans.","volume":"17","author":"Chen","year":"2021","journal-title":"PLoS Pathogens"},{"key":"B4","doi-asserted-by":"publisher","first-page":"1730","DOI":"10.14309\/ajg.0000000000002968","article-title":"ACG clinical guideline: Treatment of Helicobacter pylori infection.","volume":"119","author":"Chey","year":"2024","journal-title":"Am. 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