{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T16:22:12Z","timestamp":1791649332131,"version":"4.3.4"},"reference-count":83,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T00:00:00Z","timestamp":1772409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    is an opportunistic pathogen causing severe hospital-acquired infections, and it rapidly acquires multidrug resistance. Its robust biofilm formation further complicates treatment and drives interest in phage therapy. A phage UHKP was isolated from hospital sewage using an MDR\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    strain (\n                    <jats:italic>KP-<\/jats:italic>\n                    03). UHKP formed clear plaques and having phage titer 2.3 \u00d7 10\n                    <jats:sup>9<\/jats:sup>\n                    PFU\/mL. Host-range testing on 19 clinical isolates showed a narrow spectrum. Only four MDR strains (\n                    <jats:italic>KP-<\/jats:italic>\n                    03,\n                    <jats:italic>KP-<\/jats:italic>\n                    05,\n                    <jats:italic>KP-<\/jats:italic>\n                    08,\n                    <jats:italic>KP-<\/jats:italic>\n                    11) and one K-17 serotype were lysed, with no activity on other strains or species. One-step growth analysis yielded a 30 min latent period and 85 PFU burst size. In planktonic culture, UHKP at MOI 1 stopped bacterial growth by 4 h and cleared cultures by 8 h, whereas at MOI 0.1 killing was delayed and incomplete. In static biofilm assays, UHKP eradicated 98% of 24-h and 96% of 48-h biofilm biomass by 24 h (MOI 1). Clearance of 72 \u2013 96 h biofilms was limited (\u2264 87% by 24 h). UHKP possesses an icosahedral head of 56 \u00b1 3 nm and a short, non-contractile tail measuring around 15 \u00b1 2 nm. Genome sequencing revealed a 62,542 bp dsDNA genome (56.6% GC) encoding 77 ORFs, and phylogenetic analysis placed UHKP in the genus\n                    <jats:italic>Lastavirus<\/jats:italic>\n                    . UHKP carries no lysogeny, toxin or antibiotic-resistance genes.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1775638","type":"journal-article","created":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T06:32:41Z","timestamp":1772433161000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Genomic and functional characterization of a lytic Klebsiella phage UHKP with antibiofilm activity"],"prefix":"10.3389","volume":"17","author":[{"given":"Muhammad","family":"Hassan","sequence":"first","affiliation":[{"name":"Department of Medical Lab Technology, The University of Haripur","place":["Haripur, Khyber Pakhtunkhwa, Pakistan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iqbal Ahmad","family":"Alvi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Afghan International Islamic University","place":["Kabul, Afghanistan"]},{"name":"Department of Microbiology, Hazara University Mansehra","place":["Mansehra, Khyber Pakhtunkhwa, Pakistan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadiq Noor","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Medical Lab Technology, The University of Haripur","place":["Haripur, Khyber Pakhtunkhwa, Pakistan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dawood","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Department of Medical Lab Technology, The University of Haripur","place":["Haripur, Khyber Pakhtunkhwa, Pakistan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Asif","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Government College University Lahore","place":["Lahore, Punjab, Pakistan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Afshan","family":"Saleem","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Haripur","place":["Haripur, Khyber Pakhtunkhwa, Pakistan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,3,2]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1175","DOI":"10.3390\/v13061175","article-title":"Improving phage-biofilm in vitro experimentation","volume":"13","author":"Abedon","year":"2021","journal-title":"Viruses"},{"key":"B2","doi-asserted-by":"publisher","first-page":"517","DOI":"10.1093\/nar\/gkz935","article-title":"CARD 2020: antibiotic resistome surveillance with the comprehensive antibiotic resistance database","volume":"48","author":"Alcock","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"B3","doi-asserted-by":"publisher","first-page":"1289","DOI":"10.1007\/s00705-020-04601-x","article-title":"RLP, a bacteriophage of the family Podoviridae, rescues mice from bacteremia caused by multi-drug-resistant Pseudomonas aeruginosa","volume":"165","author":"Alvi","year":"2020","journal-title":"Arch. 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