{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T06:58:53Z","timestamp":1775804333194,"version":"3.50.1"},"reference-count":108,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T00:00:00Z","timestamp":1775779200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    The use of beneficial microbes to reduce plant stress upon fungal pathogens is a promising strategy for sustainable crop protection. In this study, we evaluated five novel\n                    <jats:italic>Burkholderia<\/jats:italic>\n                    strains for their capacity to mitigate\n                    <jats:italic>Botrytis cinerea<\/jats:italic>\n                    infection in tomato (\n                    <jats:italic>Solanum lycopersicum<\/jats:italic>\n                    L.) and\n                    <jats:italic>Fusarium graminearum<\/jats:italic>\n                    infection in wheat (\n                    <jats:italic>Triticum aestivum<\/jats:italic>\n                    L.). All strains significantly suppressed\n                    <jats:italic>B. cinerea<\/jats:italic>\n                    infection symptoms in tomato plants, hallmarked by a smaller decrease in photosynthetic activity and chlorophyll content compared to infected control. Using a GFP-tagged\n                    <jats:italic>F. graminearum<\/jats:italic>\n                    strain, we show that the strains reduced fungal biomass accumulation in\n                    <jats:italic>F. graminearum<\/jats:italic>\n                    -infected wheat leaves and mitigated chlorosis. Phytotoxicity assessments revealed no adverse effects in tomato for any strain, while two strains induced a mild reduction in chlorophyll fluorescence in wheat, suggesting potential host-specific phytotoxicity. Whole-genome sequencing of the\n                    <jats:italic>Burkholderia<\/jats:italic>\n                    strains revealed a rich repertoire of biosynthetic gene clusters (BGCs) with conserved replicon positioning. While all strains shared BGCs for known bioactive metabolites such as occidiofungin A, ornibactin, and pyochelin, variation in other clusters did not directly correlate with the phenotypic effects observed. Our results highlight the strong biocontrol potential\n                    <jats:italic>Burkholderia<\/jats:italic>\n                    strains in two economically important crops against two globally important fungal pathounder controlled conditions. Finally, a microbial phylogenomic analysis revealed that the five strains belong to two new and previously uncharacterized species within the\n                    <jats:italic>Burkholderia cepacia<\/jats:italic>\n                    complex, for which the names\n                    <jats:italic>Burkholderia mycopellens<\/jats:italic>\n                    sp. nov. and\n                    <jats:italic>Burkholderia crassaminum<\/jats:italic>\n                    sp. nov. are proposed. These strains hold promise as next-generation biocontrol agents for enhancing crop health and managing fungal diseases sustainably.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1776517","type":"journal-article","created":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T06:03:43Z","timestamp":1775801023000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Biological control of Botrytis cinerea in tomato and Fusarium graminearum in wheat using the novel species Burkholderia mycopellens and Burkholderia crassaminum"],"prefix":"10.3389","volume":"17","author":[{"given":"Ayesha","family":"Kousar","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology, Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University","place":["Ghent, Belgium"]},{"name":"Laboratory of Applied Mycology and Phenomics, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University","place":["Ghent, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eliza","family":"Depoorter","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University","place":["Ghent, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charlotte","family":"Peeters","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University","place":["Ghent, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kurt","family":"Houf","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University","place":["Ghent, Belgium"]},{"name":"Department of Veterinary and Biosciences, Faculty of Veterinary Medicine, Ghent University","place":["Merelbeke, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kris","family":"Audenaert","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Mycology and Phenomics, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University","place":["Ghent, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Vandamme","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University","place":["Ghent, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"No\u00e9mie","family":"De Zutter","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Mycology and Phenomics, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University","place":["Ghent, Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,4,10]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1007\/s10526-024-10284-z","article-title":"Burkholderia gladioli BNM349 as a promising source of bacterial metabolites for biocontrol of common bacterial blight of bean","volume":"70","author":"Alvarez","year":"2025","journal-title":"BioControl"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1007\/s11274-022-03299-0","article-title":"Genome mining of Burkholderia ambifaria strain T16, a rhizobacterium able to produce antimicrobial compounds and degrade the mycotoxin fusaric acid","volume":"38","author":"Alvarez","year":"2022","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref120","doi-asserted-by":"publisher","first-page":"1671","DOI":"10.1104\/pp.15.00107","article-title":"Priming of wheat with the green leaf volatile Z-3-Hexenyl Acetate enhances defense against Fusarium graminearum but boosts Deoxynivalenol production","volume":"167","author":"Ameye","year":"2015","journal-title":"Plant Physiol."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"906724","DOI":"10.3389\/fmicb.2022.906724","article-title":"Burkholderia ambifaria XN08: a plant growth-promoting endophytic bacterium with biocontrol potential against sharp eyespot in wheat","volume":"13","author":"An","year":"2022","journal-title":"Front. 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Soc."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"W46","DOI":"10.1093\/nar\/gkad344","article-title":"antiSMASH 7.0: new and improved predictions for detection, regulation, chemical structures and visualisation","volume":"51","author":"Blin","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1023\/b:ejpp.0000010143.20226.21","article-title":"Development and evaluation of an in vitro detached leaf assay forc pre-screening resistance to fusarium head blight in wheat","volume":"110","author":"Browne","year":"2004","journal-title":"Eur. J. Plant Pathol."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"4139","DOI":"10.1128\/aem.66.9.4139-4141.2000","article-title":"Identification and characteristics of a novel Burkholderia strain with broad-spectrum antimicrobial activity","volume":"66","author":"Cain","year":"2000","journal-title":"Appl. Environ. 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Sci."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"Fastp: an ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"ref19","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1016\/j.tim.2006.04.006","article-title":"Burkholderia cepacia complex species: health hazards and biotechnological potential","volume":"14","author":"Chiarini","year":"2006","journal-title":"Trends Microbiol."},{"key":"ref20","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1099\/ijsem.0.002516","article-title":"Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes","volume":"68","author":"Chun","year":"2018","journal-title":"Int. J. Syst. Evol. 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Biotechnol."},{"key":"ref26","doi-asserted-by":"publisher","first-page":"e01169-21","DOI":"10.1128\/AEM.01169-21","article-title":"Biosynthesis of Ditropolonyl sulfide, an antibacterial compound produced by Burkholderia cepacia complex strain R-12632","volume":"87","author":"Depoorter","year":"","journal-title":"Appl. Environ. Microbiol."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"147","DOI":"10.3390\/antibiotics10020147","article-title":"Burkholderia bacteria produce multiple potentially novel molecules that inhibit carbapenem-resistant gram-negative bacterial pathogens","volume":"10","author":"Depoorter","year":"","journal-title":"Antibiotics"},{"key":"ref28","doi-asserted-by":"publisher","first-page":"1594","DOI":"10.3389\/fmicb.2020.01594","article-title":"Burkholderia cepacia complex taxon K: where to Split?","volume":"11","author":"Depoorter","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref29","doi-asserted-by":"publisher","first-page":"1007","DOI":"10.12688\/f1000research.8221.1","article-title":"Members of the genus Burkholderia: good and bad guys","volume":"5","author":"Eberl","year":"2016","journal-title":"F1000Res"},{"key":"ref30","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1046\/j.1365-2672.1998.00473.x","article-title":"Pyrrolnitrin from Burkholderia cepacia: antibiotic activity against fungi and novel activities against streptomycetes","volume":"85","author":"el-Banna","year":"1998","journal-title":"J. Appl. Microbiol."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"321","DOI":"10.3390\/metabo11050321","article-title":"An overview of metabolic activity, beneficial and pathogenic aspects of Burkholderia spp","volume":"11","author":"Elshafie","year":"2021","journal-title":"Meta"},{"key":"ref32","doi-asserted-by":"publisher","first-page":"179","DOI":"10.24271\/psr.2024.420817.1408","article-title":"Molecular identification, prevalence, and phylogeny of Burkholderia cepacia complex (BCC) species in the respiratory tract of hospitalized patients","volume":"6","author":"Esho","year":"2024","journal-title":"Passer J. Basic Appl. Sci."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"512","DOI":"10.1002\/mbo3.347","article-title":"Burkholderia genome mining for nonribosomal peptide synthetases reveals a great potential for novel siderophores and lipopeptides synthesis","volume":"5","author":"Esmaeel","year":"2016","journal-title":"MicrobiologyOpen"},{"key":"ref34","doi-asserted-by":"publisher","first-page":"943","DOI":"10.1016\/s0953-7562(09)80091-9","article-title":"Genetic basis of resistance to benzimidazole and dicarboximide fungicides in Botryotinia fuckeliana (Botrytis cinerea)","volume":"95","author":"Faretra","year":"1991","journal-title":"Mycol. Res."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"807","DOI":"10.1080\/17460441.2021.1877655","article-title":"Drug discovery through the isolation of natural products from Burkholderia","volume":"16","author":"Foxfire","year":"2021","journal-title":"Expert Opin. Drug Discov."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1078\/0176-1617-00887","article-title":"Relationships between leaf chlorophyll content and spectral reflectance and algorithms for non-destructive chlorophyll assessment in higher plant leaves","volume":"160","author":"Gitelson","year":"2003","journal-title":"J. Plant Physiol."},{"key":"ref37","doi-asserted-by":"publisher","first-page":"e43","DOI":"10.1002\/imt2.43","article-title":"Complex heatmap visualization","volume":"1","author":"Gu","year":"2022","journal-title":"iMeta"},{"key":"ref38","doi-asserted-by":"publisher","first-page":"1072","DOI":"10.1093\/bioinformatics\/btt086","article-title":"QUAST: quality assessment tool for genome assemblies","volume":"29","author":"Gurevich","year":"2013","journal-title":"Bioinformatics"},{"key":"ref39","doi-asserted-by":"publisher","first-page":"1143","DOI":"10.3390\/antibiotics11091143","article-title":"Occidiofungin: actin binding as a novel mechanism of action in an antifungal agent","volume":"11","author":"Hansanant","year":"2022","journal-title":"Antibiotics"},{"key":"ref40","doi-asserted-by":"publisher","first-page":"1073","DOI":"10.1128\/JCM.39.3.1073-1078.2001","article-title":"Phenotypic methods for determining genomovar status of the Burkholderia cepacia complex","volume":"39","author":"Henry","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"619","DOI":"10.3390\/biology11040619","article-title":"Biological control activity of plant growth promoting rhizobacteria Burkholderia contaminans AY001 against tomato fusarium wilt and bacterial speck diseases","volume":"11","author":"Heo","year":"2022","journal-title":"Biology"},{"key":"ref42","doi-asserted-by":"publisher","first-page":"481","DOI":"10.1094\/PHYTO-06-21-0225-R","article-title":"Occidiofungin is the key metabolite for antifungal activity of the endophytic bacterium Burkholderia sp. MS455 against aspergillus flavus","volume":"112","author":"Jia","year":"2022","journal-title":"Phytopathology"},{"key":"ref43","doi-asserted-by":"publisher","first-page":"124","DOI":"10.12688\/wellcomeopenres.14826.1","article-title":"Open-access bacterial population genomics: BIGSdb software, the PubMLST. Org website and their applications","volume":"3","author":"Jolley","year":"2018","journal-title":"Wellcome Open Res."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1007\/s13765-018-0345-9","article-title":"Isolation of Burkholderia cepacia JBK9 with plant growth-promoting activity while producing pyrrolnitrin antagonistic to plant fungal diseases","volume":"61","author":"Jung","year":"2018","journal-title":"Appl. Biol. Chem."},{"key":"ref45","doi-asserted-by":"publisher","first-page":"896","DOI":"10.1139\/w11-081","article-title":"Biological control of Botrytis cinerea using the antagonistic and endophytic Burkholderia cepacia Cs5 for vine plantlet protection","volume":"57","author":"Kilani-Feki","year":"2011","journal-title":"Can. J. Microbiol."},{"key":"ref46","doi-asserted-by":"publisher","first-page":"1302236","DOI":"10.3389\/fmicb.2023.1302236","article-title":"Insights into group-specific pattern of secondary metabolite gene cluster in Burkholderia genus","volume":"14","author":"Kim","year":"2024","journal-title":"Front. Microbiol."},{"key":"ref47","doi-asserted-by":"publisher","first-page":"540","DOI":"10.1038\/s41587-019-0072-8","article-title":"Assembly of long, error-prone reads using repeat graphs","volume":"37","author":"Kolmogorov","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref48","doi-asserted-by":"publisher","first-page":"2018","DOI":"10.1021\/acs.jnatprod.8b01068","article-title":"Burkholderia as a source of natural products","volume":"82","author":"Kunakom","year":"2019","journal-title":"J. Nat. Prod."},{"key":"ref49","doi-asserted-by":"publisher","first-page":"1100","DOI":"10.1099\/ijsem.0.000760","article-title":"OrthoANI: an improved algorithm and software for calculating average nucleotide identity","volume":"66","author":"Lee","year":"2016","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref50","doi-asserted-by":"publisher","first-page":"59","DOI":"10.5423\/ppj.2011.27.1.059","article-title":"Isolation and identification of Burkholderia pyrrocinia CH-67 to control tomato leaf mold and damping-off on crisphead lettuce and tomato","volume":"27","author":"Lee","year":"2011","journal-title":"Plant Pathol. J."},{"key":"ref51","doi-asserted-by":"publisher","first-page":"W293","DOI":"10.1093\/nar\/gkab301","article-title":"Interactive tree of life (iTOL) v5: an online tool for phylogenetic tree display and annotation","volume":"49","author":"Letunic","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref52","doi-asserted-by":"publisher","first-page":"2103","DOI":"10.1093\/bioinformatics\/btw152","article-title":"Minimap and miniasm: fast mapping and de novo assembly for noisy long sequences","volume":"32","author":"Li","year":"2016","journal-title":"Bioinformatics"},{"key":"ref53","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1007\/s11104-020-04441-3","article-title":"Effect of wheat cultivars with different resistance to fusarium head blight on rhizosphere fusarium graminearum abundance and microbial community composition","volume":"448","author":"Li","year":"2020","journal-title":"Plant Soil"},{"key":"ref54","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1016\/s0261-2194(01)00074-6","article-title":"Broad spectrum anti-biotic activity and disease suppression by the potential biocontrol agent Burkholderia ambifaria BC-F","volume":"21","author":"Li","year":"2002","journal-title":"Crop Prot."},{"key":"ref55","doi-asserted-by":"publisher","first-page":"11097","DOI":"10.3390\/ijms252011097","article-title":"Volatile organic compounds produced by co-culture of Burkholderia vietnamiensis B418 with Trichoderma harzianum T11-W exhibits improved antagonistic activities against fungal phytopathogens","volume":"25","author":"Li","year":"2024","journal-title":"Int. J. Mol. Sci."},{"key":"ref56","doi-asserted-by":"publisher","first-page":"745","DOI":"10.3390\/molecules26030745","article-title":"Fungicidal activity of volatile organic compounds emitted by Burkholderia gladioli strain BBB-01","volume":"26","author":"Lin","year":"2021","journal-title":"Molecules"},{"key":"ref57","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13568-020-01032-4","article-title":"Identification, cloning and expression patterns of the genes related to phosphate solubilization in Burkholderia multivorans WS-FJ9 under different soluble phosphate levels","volume":"10","author":"Liu","year":"2020","journal-title":"AMB Express"},{"key":"ref58","doi-asserted-by":"publisher","first-page":"8312","DOI":"10.1021\/bi900814c","article-title":"Occidiofungin, a unique antifungal glycopeptide produced by a strain of Burkholderia contaminans","volume":"48","author":"Lu","year":"2009","journal-title":"Biochemistry"},{"key":"ref59","doi-asserted-by":"publisher","first-page":"737","DOI":"10.1007\/s12355-019-00703-2","article-title":"Beneficial linkages of endophytic Burkholderia anthina MYSP113 towards sugarcane growth promotion","volume":"21","author":"Malviya","year":"2019","journal-title":"Sugar Tech."},{"key":"ref60","doi-asserted-by":"publisher","first-page":"121","DOI":"10.3390\/ijms20010121","article-title":"Genomic features and insights into the taxonomy, virulence, and benevolence of plant-associated Burkholderia species","volume":"20","author":"Mannaa","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"ref61","doi-asserted-by":"publisher","first-page":"294","DOI":"10.1094\/PDIS.1998.82.3.294","article-title":"Seed treatment using pre-infiltration and biocontrol agents to reduce damping-off of corn caused by species of Pythium and fusarium","volume":"82","author":"Mao","year":"1998","journal-title":"Plant Dis."},{"key":"ref62","doi-asserted-by":"publisher","first-page":"639","DOI":"10.1038\/nchembio.1884","article-title":"Computational approaches to natural product discovery","volume":"11","author":"Medema","year":"2015","journal-title":"Nat. Chem. Biol."},{"key":"ref63","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1186\/1471-2105-14-60","article-title":"Genome sequence-based species delimitation with confidence intervals and improved distance functions","volume":"14","author":"Meier-Kolthoff","year":"","journal-title":"BMC Bioinformatics"},{"key":"ref64","doi-asserted-by":"publisher","first-page":"2182","DOI":"10.1038\/s41467-019-10210-3","article-title":"TYGS is an automated high-throughput platform for state-of-the-art genome-based taxonomy","volume":"10","author":"Meier-Kolthoff","year":"2019","journal-title":"Nat. Commun."},{"key":"ref65","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1007\/s00203-013-0888-4","article-title":"When should a DDH experiment be mandatory in microbial taxonomy?","volume":"195","author":"Meier-Kolthoff","year":"","journal-title":"Arch. 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Biol."},{"key":"ref69","doi-asserted-by":"publisher","first-page":"127201","DOI":"10.1016\/j.micres.2022.127201","article-title":"Endophytic Burkholderia: multifunctional roles in plant growth promotion and stress tolerance","volume":"265","author":"Pal","year":"2022","journal-title":"Microbiol. 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Microbiol."},{"key":"ref82","doi-asserted-by":"publisher","first-page":"baaa062","DOI":"10.1093\/database\/baaa062","article-title":"NCBI taxonomy: a comprehensive update on curation, resources and tools","volume":"2020","author":"Schoch","year":"2020","journal-title":"Database"},{"key":"ref83","doi-asserted-by":"publisher","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","article-title":"Prokka: rapid prokaryotic genome annotation","volume":"30","author":"Seemann","year":"2014","journal-title":"Bioinformatics"},{"key":"ref84","doi-asserted-by":"publisher","first-page":"418","DOI":"10.1002\/jobm.201200770","article-title":"Antifungal attributes of siderophore produced by the Pseudomonas aeruginosa JAS-25","volume":"54","author":"Sulochana","year":"2014","journal-title":"J. Basic Microbiol."},{"key":"ref85","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1038\/ja.2008.58","article-title":"Novel oxidized derivatives of antifungal pyrrolnitrin from the bacterium Burkholderia cepacia K87","volume":"61","author":"Sultan","year":"2008","journal-title":"J. Antibiot."},{"key":"ref86","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1016\/j.micres.2018.05.004","article-title":"Effectiveness of multi-trait Burkholderia contaminans KNU17BI1 in growth promotion and management of banded leaf and sheath blight in maize seedling","volume":"214","author":"Tagele","year":"2018","journal-title":"Microbiol. Res."},{"key":"ref87","doi-asserted-by":"publisher","first-page":"1005","DOI":"10.3390\/ijms20051005","article-title":"Potential of novel sequence type of Burkholderia cenocepacia for biological control of root rot of maize (Zea mays L.) caused by fusarium temperatum","volume":"20","author":"Tagele","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"ref88","doi-asserted-by":"publisher","first-page":"1559","DOI":"10.1111\/mpp.12996","article-title":"At the scene of the crime: new insights into the role of weakly pathogenic members of the fusarium head blight disease complex","volume":"21","author":"Tan","year":"2020","journal-title":"Mol. Plant Pathol."},{"key":"ref89","doi-asserted-by":"publisher","first-page":"641890","DOI":"10.3389\/fpls.2021.641890","article-title":"Presence of the weakly pathogenic fusarium poae in the fusarium head blight disease complex hampers biocontrol and chemical control of the virulent fusarium graminearum pathogen","volume":"12","author":"Tan","year":"2021","journal-title":"Front. 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Microbiol."},{"key":"ref99","doi-asserted-by":"publisher","first-page":"e04805-04822","DOI":"10.1128\/spectrum.04805-22","article-title":"Insights into the biocontrol function of a Burkholderia gladioli strain against Botrytis cinerea","volume":"11","author":"Wang","year":"2023","journal-title":"Microbiol. 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