{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T08:16:12Z","timestamp":1775117772265,"version":"3.50.1"},"reference-count":69,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T00:00:00Z","timestamp":1775088000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    We describe a rapidly growing mycobacterial (RGM) species closely related to\n                    <jats:italic>Mycobacterium wolinskyi<\/jats:italic>\n                    . The bacterium was isolated from three biopsies obtained from a 78-year-old Venezuelan woman who presented with a chronic superficial erythematous plaque in the right nasal cavity. The three isolates, LTG2003(1\u20133), exhibited identical phenotypic, biochemical, and molecular characteristics and showed susceptibility to amikacin and linezolid, intermediate susceptibility to cefoxitin and imipenem, and resistance to ciprofloxacin, clarithromycin, sulfamethoxazole-trimethoprim, and tobramycin. Conventional diagnostic methods, including biochemical assays,\n                    <jats:italic>hsp65<\/jats:italic>\n                    PCR restriction enzyme analysis (PRA-\n                    <jats:italic>hsp65<\/jats:italic>\n                    ), the Genotype Mycobacterium CM v2.0 linear probe assay, and Sanger sequencing of the\n                    <jats:italic>16S rRNA<\/jats:italic>\n                    ,\n                    <jats:italic>hsp65<\/jats:italic>\n                    , and\n                    <jats:italic>rpoB<\/jats:italic>\n                    genes, failed to identify the microorganism to the species level. MALDI-TOF mass spectrometry yielded genus-level scores suggestive of\n                    <jats:italic>M. wolinskyi<\/jats:italic>\n                    . Whole-genome sequencing confirmed that the isolates represent a new species. The type strain, designated LTG 2003-1 (WFCC 1312\u202f=\u202fP7483\u202f=\u202fCDBB-500-3), has been deposited in two internationally recognized microbial culture collections in Mexico and Brazil. The genome of this organism has also been deposited in GenBank as\n                    <jats:italic>Mycobacterium<\/jats:italic>\n                    sp. LTG2003 with accession number JBONDW000000000. Based on our findings, we propose that the LTG2003(1-3) isolates represent a new species, for which we propose the name\n                    <jats:italic>Mycobacterium venezuelense<\/jats:italic>\n                    sp.\n                    <jats:italic>nov<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1780266","type":"journal-article","created":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T07:42:37Z","timestamp":1775115757000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Description and complete genome sequence of Mycobacterium venezuelense sp. nov., a rapidly growing species recovered from a soft tissue infection"],"prefix":"10.3389","volume":"17","author":[{"given":"Yrneh Yadamis Prado","family":"Palacios","sequence":"first","affiliation":[{"name":"Departamento de Tuberculosis y Micobacteriosis, Instituto de Biomedicina Dr. Jacinto Convit, Universidad Central de Venezuela","place":["Caracas, Venezuela"]},{"name":"Laborat\u00f3rio de Bacteriologia e Bioensaios em Tuberculose e outras Micobact\u00e9rias (LBBTM), Instituto Nacional de Infectologia Evandro Chagas, Instituto Oswaldo Cruz","place":["Rio de Janeiro, Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonardo Bruno Paz Ferreira","family":"Barreto","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Bacteriologia e Bioensaios em Tuberculose e outras Micobact\u00e9rias (LBBTM), Instituto Nacional de Infectologia Evandro Chagas, Instituto Oswaldo Cruz","place":["Rio de Janeiro, Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Carolina","family":"Sisco","sequence":"additional","affiliation":[{"name":"Departamento de Tuberculosis y Micobacteriosis, Instituto de Biomedicina Dr. Jacinto Convit, Universidad Central de Venezuela","place":["Caracas, Venezuela"]},{"name":"Laborat\u00f3rio Nacional de Computa\u00e7\u00e3o Cient\u00edfica, Minist\u00e9rio da Ci\u00eancia, Tecnologia, Inova\u00e7\u00e3o e Comunica\u00e7\u00f5es","place":["Petr\u00f3polis, Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emilyn Costa","family":"Concei\u00e7\u00e3o","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Bacteriologia e Bioensaios em Tuberculose e outras Micobact\u00e9rias (LBBTM), Instituto Nacional de Infectologia Evandro Chagas, Instituto Oswaldo Cruz","place":["Rio de Janeiro, Brazil"]},{"name":"SAMRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University","place":["Cape Town, South Africa"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abhinav","family":"Sharma","sequence":"additional","affiliation":[{"name":"SAMRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University","place":["Cape Town, South Africa"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco","family":"das Chagas de Sousa","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Bacteriologia e Bioensaios em Tuberculose e outras Micobact\u00e9rias (LBBTM), Instituto Nacional de Infectologia Evandro Chagas, Instituto Oswaldo Cruz","place":["Rio de Janeiro, Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guilherme","family":"da Silva Louren\u00e7o Carvalho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Bacteriologia e Bioensaios em Tuberculose e outras Micobact\u00e9rias (LBBTM), Instituto Nacional de Infectologia Evandro Chagas, Instituto Oswaldo Cruz","place":["Rio de Janeiro, Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisy Anne","family":"Barbosa de Lira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Bacteriologia e Bioensaios em Tuberculose e outras Micobact\u00e9rias (LBBTM), Instituto Nacional de Infectologia Evandro Chagas, Instituto Oswaldo Cruz","place":["Rio de Janeiro, Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruna","family":"Moreira de Sousa Melo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Bacteriologia e Bioensaios em Tuberculose e outras Micobact\u00e9rias (LBBTM), Instituto Nacional de Infectologia Evandro Chagas, Instituto Oswaldo Cruz","place":["Rio de Janeiro, Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo","family":"P\u00e9rez-Alfonzo","sequence":"additional","affiliation":[{"name":"Centro Cl\u00ednico de Dermatolog\u00eda y Enfermedades Tropicales, Instituto de Biomedicina Dr. Jacinto Convit, Universidad Central de Venezuela","place":["Caracas, Venezuela"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier E.","family":"Ortega Diaz","sequence":"additional","affiliation":[{"name":"Centro Cl\u00ednico de Dermatolog\u00eda y Enfermedades Tropicales, Instituto de Biomedicina Dr. Jacinto Convit, Universidad Central de Venezuela","place":["Caracas, Venezuela"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franklin E.","family":"Claro Almea","sequence":"additional","affiliation":[{"name":"Departamento de Tuberculosis y Micobacteriosis, Instituto de Biomedicina Dr. Jacinto Convit, Universidad Central de Venezuela","place":["Caracas, Venezuela"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas","family":"Silva","sequence":"additional","affiliation":[{"name":"Departamento de Tuberculosis y Micobacteriosis, Instituto de Biomedicina Dr. Jacinto Convit, Universidad Central de Venezuela","place":["Caracas, Venezuela"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edson","family":"Machado","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia 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G.","family":"Vasconcellos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular aplicada a Micobact\u00e9rias, Instituto Oswaldo Cruz","place":["Rio de Janeiro, Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip","family":"Suffys","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular aplicada a Micobact\u00e9rias, Instituto Oswaldo Cruz","place":["Rio de Janeiro, Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Cristina da Silva Louren\u00e7o","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Bacteriologia e Bioensaios em Tuberculose e outras Micobact\u00e9rias (LBBTM), Instituto Nacional de Infectologia Evandro Chagas, Instituto Oswaldo Cruz","place":["Rio de Janeiro, Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacobus H.","family":"de Waard","sequence":"additional","affiliation":[{"name":"Departamento de Tuberculosis y Micobacteriosis, Instituto de Biomedicina Dr. Jacinto Convit, Universidad Central de Venezuela","place":["Caracas, Venezuela"]},{"name":"Facultad de Medicina, Universidad Esp\u00edritu Santo","place":["Samborond\u00f3n, Ecuador"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,4,2]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1016\/j.diagmicrobio.2011.08.005","volume":"71","author":"Ariza-Heredia","year":"2011","journal-title":"Mycobacterium wolinskyi: a case series and review of the literature. 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