{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T03:16:16Z","timestamp":1777864576389,"version":"3.51.4"},"reference-count":45,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Dengue virus serotype 2 (DENV-2) is a major driver of severe dengue outbreaks globally, yet its evolutionary history and transmission dynamics across Africa remain incompletely understood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>To clarify its origins, introduction pathways, and patterns of regional spread, we compiled all available full-length DENV-2 genomes up to 16 July 2025, prioritizing African sequences while retaining representative global references. After quality filtering and down-sampling, a final dataset of 414 genomes was analyzed. Sequences were screened for recombination using RDP4 and analyzed with maximum-likelihood phylogenetics in IQ-TREE with 1,000 ultrafast bootstrap replicates. Temporal dynamics were inferred using time-scaled phylogenies reconstructed in BEAST under a strict molecular clock and exponential-growth coalescent prior, while viral dispersal was examined through discrete-trait phylogeographic analyses with Bayesian stochastic search variable selection. In parallel, single-nucleotide polymorphisms in the prM, E, and NS1 genes were characterized, and significant sites were mapped onto corresponding protein structures.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results and Discussion<\/jats:title>\n                    <jats:p>Between 1964 and 2024, African DENV-2 diversity was shaped primarily by repeated introductions of the Cosmopolitan genotype, giving rise to geographically structured lineages. Lineage IIB, likely originating from La R\u00e9union in the late 1970s, subsequently disseminated across West, Central, and East Africa, with Burkina Faso emerging as a key regional hub. Lineage IIA, also linked to La R\u00e9union, diverged into two sub-lineages that expanded through East and Central Africa and extended into South Asia. In contrast, lineages IID and IIF were detected only sporadically, with limited geographic representation and no evidence of sustained regional expansion. Despite substantial genetic variation across prM, E, and NS1, relatively few significant non-synonymous mutations were identified, and critical N-glycosylation motifs remained conserved. This pattern suggests that epidemiological connectivity and repeated viral introductions, rather than sustained positive selection at major functional loci, are the dominant forces shaping DENV-2 spread in Africa. Overall, our findings highlight the central role of recurrent introductions and regional transmission networks in structuring DENV-2 circulation across the continent and emphasize the need for strengthened genomic surveillance to track lineage emergence and improve evidence-based dengue prevention and control strategies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmicb.2026.1782819","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T05:44:08Z","timestamp":1777527848000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Evolutionary history and transmission dynamics of dengue virus type 2 in Africa"],"prefix":"10.3389","volume":"17","author":[{"given":"Muhammad Bashir","family":"Bello","sequence":"first","affiliation":[{"name":"Department of Infectious Disease Research, King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, Ministry of National Guard Health Affairs","place":["Riyadh, Saudi Arabia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Safia S.","family":"Aljedani","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease Research, King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, Ministry of National Guard Health Affairs","place":["Riyadh, Saudi Arabia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nafi\u2019u","family":"Lawal","sequence":"additional","affiliation":[{"name":"Center for Advanced Medical Research and Training, Usmanu Danfodiyo University","place":["Sokoto, Nigeria"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdulrahman","family":"Alasiri","sequence":"additional","affiliation":[{"name":"Department of Artificial Intelligence and Bioinformatics, King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, Ministry of National Guard Health Affairs","place":["Riyadh, Saudi Arabia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mustapha Umar","family":"Imam","sequence":"additional","affiliation":[{"name":"Center for Advanced Medical Research and Training, Usmanu Danfodiyo University","place":["Sokoto, Nigeria"]},{"name":"Center for Vaccine Research and Biotechnology, Federal University of Lafia","place":["Lafia, Nigeria"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,4,30]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"100337","DOI":"10.1016\/j.onehlt.2021.100337","article-title":"Tracing and tracking the emergence, epidemiology and dispersal of dengue virus to Africa during the 20th century.","volume":"13","author":"Alfsnes","year":"2021","journal-title":"One Health"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1349","DOI":"10.3201\/eid1708.101515","article-title":"Dengue virus infection in Africa.","volume":"17","author":"Amarasinghe","year":"2011","journal-title":"Emerg. 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