{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T14:45:53Z","timestamp":1787496353193,"version":"build-2736575974"},"reference-count":64,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T00:00:00Z","timestamp":1782259200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    The escalating prevalence of antibiotic resistance represents a critical threat to global public health, while the development of new antibiotics remains limited. Vancomycin, a glycopeptide antibiotic, is essential for treating severe Gram-positive infections. However, the emergence of vancomycin-intermediate (VISA) and vancomycin-resistant (VRSA)\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    strains has raised significant concerns due to their challenging resistance profiles and limited treatment options. In this study, we developed a carboxylic acid-capped silver nanoparticle (AgNPs) platform to enhance the therapeutic delivery and efficacy of vancomycin. The nanocomposite (AgNPs\/Van\/CS) was engineered by leveraging electrostatic interactions between AgNPs and vancomycin, with a chitosan coating to provide stability, bacterial adhesion, and improved biocompatibility. Our results demonstrate that AgNPs\/Van\/CS significantly outperformed vancomycin alone, exhibiting enhanced antibacterial and antibiofilm activity against both methicillin-sensitive (MSSA) and methicillin-resistant (MRSA)\n                    <jats:italic>S. aureus<\/jats:italic>\n                    strains, as well as vancomycin-intermediate (VISA) clinical isolates. Notably, AgNPs\/Van\/CS achieved potent bacterial inhibition at lower antibiotic concentrations and significantly reduced biofilm formation, while maintaining strong activity under diverse laboratory conditions. These findings highlight the potential of AgNPs\/Van\/CS as a promising strategy for managing\n                    <jats:italic>S. aureus<\/jats:italic>\n                    infections and addressing the urgent challenge of antibiotic resistance.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1787175","type":"journal-article","created":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T05:47:36Z","timestamp":1782280056000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Chitosan-coated silver nanoparticles amplify glycopeptide efficacy against Staphylococcus aureus"],"prefix":"10.3389","volume":"17","author":[{"given":"Sakshi V.","family":"Khairnar","sequence":"first","affiliation":[{"name":"Center for Drug Delivery and Nanomedicine, Department of Pharmaceutical Sciences, University of Nebraska Medical Center","place":["Omaha, NE, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Sommers","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, University of Nebraska Medical Center","place":["Omaha, NE, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Muldiiarova","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, University of Nebraska Medical Center","place":["Omaha, NE, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth W.","family":"Bayles","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, University of Nebraska Medical Center","place":["Omaha, NE, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Oupick\u00fd","sequence":"additional","affiliation":[{"name":"Center for Drug Delivery and Nanomedicine, Department of Pharmaceutical Sciences, University of Nebraska Medical Center","place":["Omaha, NE, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinai C.","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, University of Nebraska Medical Center","place":["Omaha, NE, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marat R.","family":"Sadykov","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, University of Nebraska Medical Center","place":["Omaha, NE, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svetlana","family":"Romanova","sequence":"additional","affiliation":[{"name":"Center for Drug Delivery and Nanomedicine, Department of Pharmaceutical Sciences, University of Nebraska Medical Center","place":["Omaha, NE, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,24]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"7641","DOI":"10.2147\/IDR.S428103","article-title":"Methicillin resistant Staphylococcus Aureus: Molecular mechanisms underlying drug resistance development and novel strategies to combat.","volume":"16","author":"Abebe","year":"2023","journal-title":"Infect. 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