{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T17:58:31Z","timestamp":1788890311306,"version":"build-2803163510"},"reference-count":59,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T00:00:00Z","timestamp":1781136000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"abstract":"<jats:p>\n                    Advanced therapeutics such as antimicrobial peptides (AMPs) represent promising alternatives for combating antimicrobial resistance due to their diverse mechanisms of action, including membrane disruption and interference with bacterial resistance systems.\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    is recognized as a priority pathogen because of its intrinsic and acquired resistance to multiple antibiotics, largely mediated by the overexpression of efflux pump systems such as MexAB-OprM. In this study, an extensive\n                    <jats:italic>in-silico<\/jats:italic>\n                    screening approach was employed to identify AMPs capable of interacting with the MexB efflux pump protein. Molecular docking and molecular dynamics simulations identified Omiganan, SAAP-148, and their engineered variants as promising candidates with strong binding affinity toward MexB. Selected peptides were synthesized and evaluated\n                    <jats:italic>in vitro<\/jats:italic>\n                    against clinical and reference strains of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    . The peptides exhibited minimum inhibitory concentrations ranging from 8 to 64\u202f\u03bcg\/mL. Flow cytometric analysis using propidium iodide uptake and scanning electron microscopy confirmed membrane disruption in peptide-treated cells. Cytotoxicity assays demonstrated comparatively low toxicity for Omiganan and SAAP-148_SM1 in HEK293 and HepG2 cell lines. Collectively, these findings highlight the potential of rationally designed antimicrobial peptides as modulators of efflux-mediated resistance. The identified peptides provide a foundation for further optimization and\n                    <jats:italic>in vivo<\/jats:italic>\n                    evaluation as potential therapeutic agents or antibiotic adjuvants against multidrug-resistant\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    infections.\n                  <\/jats:p>","DOI":"10.3389\/fmicb.2026.1796090","type":"journal-article","created":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T05:48:45Z","timestamp":1781156925000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["From computational screening to in vitro validation: exploring antimicrobial peptides against Pseudomonas aeruginosa"],"prefix":"10.3389","volume":"17","author":[{"given":"Debolina","family":"Chatterjee","sequence":"first","affiliation":[{"name":"School of Biosciences and Technology, Vellore Institute of Technology","place":["Vellore, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Indranil","family":"Biswas","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center","place":["Kansas City, KS, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumit","family":"Mittal","sequence":"additional","affiliation":[{"name":"School of Advanced Sciences and Languages (SASL), VIT Bhopal University","place":["Sehore, Madhya Pradesh, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sreyoshi","family":"Routh","sequence":"additional","affiliation":[{"name":"School of Biosciences and Technology, Vellore Institute of Technology","place":["Vellore, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Venkatraman","family":"Manickam","sequence":"additional","affiliation":[{"name":"School of Biosciences and Technology, Vellore Institute of Technology","place":["Vellore, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazi","family":"Tanvir","sequence":"additional","affiliation":[{"name":"School of Advanced Sciences, Vellore Institute of Technology","place":["Vellore, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Karunanidhi","sequence":"additional","affiliation":[{"name":"Anthem Biosciences","place":["Bangalore, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atul","family":"Godha","sequence":"additional","affiliation":[{"name":"Anthem Biosciences","place":["Bangalore, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karthikeyan","family":"Sivashanmugam","sequence":"additional","affiliation":[{"name":"School of Biosciences and Technology, Vellore Institute of Technology","place":["Vellore, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,11]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"626","DOI":"10.3390\/antibiotics12030626","article-title":"Evaluation of efflux-mediated resistance and biofilm formation in virulent Pseudomonas aeruginosa associated with healthcare infections","volume":"12","author":"Akinduti","year":"2023","journal-title":"Antibiotics"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"703","DOI":"10.3390\/life13030703","article-title":"Antimicrobial activities and biofilm inhibition properties of Trigonella foenumgraecum methanol extracts against multidrug-resistant Staphylococcus aureus and Escherichia coli","volume":"13","author":"Alenazy","year":"2023","journal-title":"Life."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"e101840","DOI":"10.1371\/journal.pone.0101840","article-title":"Identification of natural compound inhibitors for multidrug efflux pumps of Escherichia coli and Pseudomonas aeruginosa using in silico high-throughput virtual screening and in vitro validation","volume":"9","author":"Aparna","year":"2014","journal-title":"PLoS One"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"5870","DOI":"10.3402\/ljm.v6i0.5870","article-title":"Efflux pump inhibitors (EPIs) as new antimicrobial agents against Pseudomonas aeruginosa","volume":"6","author":"Askoura","year":"2011","journal-title":"Libyan J. 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